HOPE-fixation enables improved PCR-based detection and differentiation of Mycobacterium tuberculosis complex in paraffin-embedded tissues

Pathology Research and Practice

Volumn 199, Issue 9, 2003, Pages 619-623

  Sen Gupta, Robin ^a   Hillemann, D ^a   Kubica, T ^a   Zissel, G ^b   Muller Quernheim J ^b   Galle, J ^a   Vollmer, E ^a   Goldmann, T ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Fixation; HOPE (Hepes glutamic acid buffer mediated Organic solvent Protection Effect); Mycobacteria diagnosis; Real time PCR; Spoligotyping

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; BACTERIAL DNA; BCG VACCINE; BUFFER; FORMALDEHYDE; GLUTAMIC ACID; ORGANIC SOLVENT; PARAFFIN;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DNA DEGRADATION; DNA EXTRACTION; FEMALE; INTERMETHOD COMPARISON; MOUSE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PATHOLOGY; POLYMERASE CHAIN REACTION; SCID MOUSE; SENSITIVITY ANALYSIS; TISSUE FIXATION; TISSUE PRESERVATION; TUBERCULOSIS;

EID: 0242288067     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1078/0344-0338-00470     Document Type: Article

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