Pitfalls in diagnostic molecular pathology - Significance of sampling error

Virchows Archiv

Volumn 439, Issue 4, 2001, Pages 504-511

  Heinmöller, Ernst ^a   Renke, Birgit ^a   Beyser, Kurt ^a   Dietmaier, Wolfgang ^b   Langner, Cord ^c   Rüschoff, Josef ^a  

^a KLINIKUM KASSEL   (Germany)

^b UNIVERSITY HOSPITAL REGENSBURG   (Germany)

^c RESEARCH INSTITUTE OF MOLECULAR PATHOLOGY   (Austria)

Author keywords

B cell clonality; Microsatellite instability; Molecular pathology; Sampling error; Tuberculosis

Indexed keywords

FORMALDEHYDE; PARAFFIN; BACTERIAL DNA; DNA; MICROSATELLITE DNA;

ADULT; ANALYTICAL ERROR; ARTICLE; B CELL LYMPHOMA; CASE REPORT; CELL HETEROGENEITY; COLORECTAL TUMOR; CONTROLLED STUDY; DIAGNOSTIC TEST; DISSECTION; DYSPLASIA; FROZEN SECTION; GASTROINTESTINAL BIOPSY; GENETIC DISORDER; HUMAN; HUMAN TISSUE; LABORATORY TEST; LASER; LYMPH NODE; MALE; MICROSATELLITE INSTABILITY; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RECTUM ADENOMA; SAMPLE; TISSUE FIXATION; TISSUE SECTION; TUBERCULOSIS; CELL CLONE; DIAGNOSTIC ERROR; EPIDEMIOLOGY; GENETIC PROCEDURES; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; MICROMANIPULATION; NONHODGKIN LYMPHOMA; PATHOLOGY;

CLONE CELLS; COLORECTAL NEOPLASMS; DIAGNOSTIC ERRORS; DISSECTION; DNA, BACTERIAL; DNA, NEOPLASM; GENETIC TECHNIQUES; HUMANS; LYMPHOMA, B-CELL; LYMPHOMA, NON-HODGKIN; MICROMANIPULATION; MICROSATELLITE REPEATS; MYCOBACTERIUM TUBERCULOSIS; PATHOLOGY, CLINICAL; POLYMERASE CHAIN REACTION; SELECTION BIAS; SPECIMEN HANDLING; TUBERCULOSIS;

EID: 0034741240     PISSN: 09456317     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004280100450     Document Type: Article

References (26)

1. Polymerase chain reaction detection of immunoglobulin gene rearrangement and bcl-2 translocation in archival glass slides of cytologic material
2. Microsatellite instability analysis: A multicenter study for reliability and quality control
3. A National Cancer Institute workshop on microsatellite instability for cancer detection and familiar predisposition: Development of international criteria for the determination of microsatellite instability in colorectal cancer
4. Quality assessment in diagnostic molecular pathology: Experience from a German-Austrian-Swiss multicenter trial
5. Peripheral-blood-based PCR assay to identify patients with active pulmonary tuberculosis
6. Diagnostic microsatellite instability: Definition and correlation with mismatch repair protein expression
7. PCR analysis of the immunoglobulin heavy chain gene in polyclonal processes can yield pseudo-clonal bands as an artifact of low B cell number
8. Molecular diagnosis of tuberculosis: The need for new diagnostic tools
9. Molecular technologies in pathology: Detecting mycobacterial DNA in paraffin-embedded tissue
10. DNA microsatellite instability and mismatch repair protein loss in adenomas presenting in hereditary non-polyposis colorectal cancer
11. Neoplastic progression occurs through mutator pathways in hyperplastic polyposis of the colorectum
12. PCR analysis of IgH-gene rearrangements in small lymphoid infiltrates microdissected from sections of paraffin-embedded bone marrow biopsy specimens
13. Comparison of PCR with Southern hybridisation for the routine detection of immumoglobulin heavy chain gene rearrangements
14. Microsatellite instability in adenomas as a marker for hereditary nonpolyposis colorectal cancer
15. Overview of the role of molecular methods in the diagnosis of malignant lymphomas
16. BAT-26 and BAT-40 instability in colorectal adenomas and carcinomas and germ line polymorphisms
17. Detection of microsatellite instability in human colorectal carcinomas using a non-radioactive PCR-based screening method
18. Identification of expressed genes by laser-mediated manipulation of single cells
19. Detection of clonal immunoglobulin heavy chain gene rearrangements by polymerase chain reaction in scrapings from archival hematoxylin and eosin-stained histologic sections: Implications for molecular genetic studies of focal pathologic lesions
20. Determination of B-cell clonality in paraffin-embedded endoscopic biopsy specimens of abnormal lymphocytic infiltrates and gastrointestinal lymphoma by polymerase chain reaction
21. B-cell target DNA quantity is a critical factor in the interpretation of B-cell clonality by PCR
22. Gene rearrangement in B- and T-lymphoproliferative disease detected by the polymerase chain reaction
23. Nested polymerase chain reaction for mycobacterium tuberculosis IS6110 sequence on formalin-fixed paraffin-embedded tissues with granulomatous diseases for rapid diagnosis of tuberculosis
24. Rapid method for detecting monoclonality in B cell lymphoma in lymph node aspirates using the polymerase chain reaction
25. Genomic instability occurs in colorectal carcinomas but not in adenomas
26. Detection of clonal B cells in microdissected reactive lymphoproliferations: Possible diagnostic pitfalls in PCR analysis of immunoglobulin heavy chain gene rearrangement