Comprehensive Step-by-Step Engineering of an (R)-Hydroxynitrile Lyase for Large-Scale Asymmetric Synthesis

Angewandte Chemie - International Edition

Volumn 42, Issue 39, 2003, Pages 4815-4818

  Glieder, Anton ^a   Weis, Roland ^b   Skranc, Wolfgang ^d   Poechlauer, Peter ^d   Dreveny, Ingrid ^c   Majer, Sandra ^a   Wubbolts, Marcel ^d   Schwab, Helmut ^a   Gruber, Karl ^b,c  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b RESEARCH CENTRE APPLIED BIOCATALYSIS   (Austria)

^c UNIVERSITY OF GRAZ   (Austria)

^d DSM Fine Chemicals Austria Nfg GmbH & Co KG   (Netherlands)

Author keywords

Asymmetric synthesis; C C coupling; Enzyme catalysis; Protein design; Protein engineering

Indexed keywords

CLONING; GENES; PROTEINS; STEREOCHEMISTRY; SYNTHESIS (CHEMICAL);

PROTEIN SEQUENCES;

ENZYMES;

HYDROXYNITRILE LYASE;

ARTICLE; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME ENGINEERING; ENZYME INACTIVATION; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; STEREOCHEMISTRY;

ACETONITRILES; CHLORINE; GLYCOSYLATION; HYDRO-LYASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NITRILES; PICHIA; SACCHAROMYCES CEREVISIAE;

EID: 0142183584     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200352141     Document Type: Article

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