Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris- utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis

Plant Science

Volumn 139, Issue 1, 1998, Pages 19-27

  Trummler, Klaus ^a   Roos, Jürgen ^a   Schwaneberg, Ulrich ^a   Effenberger, Franz ^a   Förster, Siegfried ^a   Pfizenmaier, Klaus ^a   Wajant, Harald ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Cyanogenesis; Hydroxynitrile lyase; Zn2+ containing alcohol dehydrogenases

Indexed keywords

ALCOHOL DEHYDROGENASE; LYASE; NITRILE; RECOMBINANT ENZYME; ZINC;

ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CATABOLISM; CATALYSIS; CLONING; ENZYME ANALYSIS; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN ANALYSIS; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; LINUM USITATISSIMUM; PICHIA PASTORIS;

EID: 0032509115     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(98)00173-3     Document Type: Article

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