House keeping genes and gene expression analysis in transplant recipients: A note of caution

Transplant Immunology

Volumn 12, Issue 1, 2003, Pages 89-97

  Gibbs, Paul J ^a   Cameron, Colin ^a   Tan, Lam Chin ^a   Sadek, Sami A ^a   Howell, W Martin ^b  

^a ST MARY S HOSPITAL   (United Kingdom)

^b SOUTHAMPTON GENERAL HOSPITAL   (United Kingdom)

Author keywords

'TaqMan' RT PCR; Gene expression; House keeping genes; Renal transplantation; Standardisation

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; COMPLEMENTARY DNA; IMMUNOSUPPRESSIVE AGENT; METHYLPREDNISOLONE; OKT 3; TRANSFERRIN RECEPTOR;

ADULT; ANALYTICAL PARAMETERS; ARTICLE; BLOOD; COHORT ANALYSIS; CONTROLLED STUDY; DNA DETERMINATION; DONOR; DRUG DOSE REGIMEN; FEMALE; GENE EXPRESSION; GRAFT REJECTION; HOUSEKEEPING GENE; HUMAN; IMMUNOSUPPRESSIVE TREATMENT; KIDNEY BIOPSY; KIDNEY DISEASE; KIDNEY TRANSPLANTATION; MAJOR CLINICAL STUDY; MALE; MONONUCLEAR CELL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

EID: 0142182172     PISSN: 09663274     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0966-3274(03)00010-8     Document Type: Article

References (46)

1. Tan L, Howell WM, Smith JL, Sadek SA. Sequential monitoring of peripheral T-lymphocyte cytokine gene expression in the early post renal allograft period. Transplantation 2001;71:751-759.
2. P Gibbs, L Tan, S Sadek, W Howell. Comparative evaluation of 'TaqMan' RT-PCR and RT-PCR ELISA for immunological monitoring of renal transplant recipients. Transplant Immunol 2002, in press.
3. Galvan B, Christopoulos TK. Quantitative reverse transcriptase-polymerase chain reaction for prostate-specific antigen. Clin Biochem 1997;30:391-397.
4. Willey JC, Crawford EL, Jackson CM, et al. Expression measurement of many genes simultaneously by quantitative RT-PCR using standardized mixtures of competitive templates. Am J Respir Cell Mol Biol 1998;19:3-5.
5. Chang TJ, Juang CC, Yin PH, Chi CW, Tsay HJ. Up-regulation of β-actin, cyclophilin and GAPDH in N1S1 rat hepatoma. Oncol Rep 1998;5:469-471.
6. El-Naggar MM, Ukai K, Takeuchi K, Sakakura Y. Expression of interferon-gamma, interleukin-4 and interleukin-5 mRNA in the nasal mucosal membrane of rats with allergic rhinitis. Scand J Immunol 1998;47:554-560.
7. Overbergh L, Valckx D, Waer M, Mathieu C. Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR. Cytokine 1999;11:305-312.
8. Mao D, Lee JK, Van Vickle SJ, Thompson RW. Expression of collagenase-3 (MMP-13) in human abdominal aortic aneurysms and vascular smooth muscle cells in culture. Biochem Biophys Res Commun 1999;261:904-910.
9. Halminen M, Sjoroos M, Makela MJ, et al. Simultaneous detection of IFN-gamma and IL-4 mRNAs using RT-PCR and time-resolved fluorometry. Cytokine 1999;11:87-93.
10. Suzuki T, Higgins PJ, Crawford DR. Control selection for RNA quantitation. BioTechniques 2000;29:332-337.
11. Sharma VK, Bologa RM, Xu GP, et al. Intragraft TGF-beta1 mRNA: A correlate of interstitial fibrosis and chronic allograft nephropathy. Kidney Int 1996;49:1297-1303.
12. Gorzynski RM, Adams RB, Levy GA, Chung SW. Correlation of peripheral blood lymphocyte and intragraft cytokine mRNA expression with rejection in orthotopic liver transplantation. Surgery 1996;120:496-502.
13. Iacono A, Dauber J, Keenan R, et al. Interleukin 6 and interferon-β gene expression in lung transplant recipients with refractory acute cellular rejection. Implications for monitoring and inhibition by treatment with aerosolized cyclosporine. Transplantation 1997;64:263-269.
14. Rokahr KL, Sharland AF, Sun J, et al. Paradoxical early immune activation during acceptance of liver allografts compared with rejection of skin grafts in a rat model of transplantation. Immunology 1998;95:257-263.
15. Naar JD, Fisher RA, Saggi BH, et al. The immunological role of IFN-gamma in ACI to Lewis kidney transplantation. J Surg Res 1998;78:103-107.
16. Flach R, Speidel N, Flohe S, et al. Analysis of intragraft cytokine expression during early reperfusion after liver transplantation using semi-quantitative RT-PCR. Cytokine 1998;10:445-451.
17. Schiano TD, Fiel MI, Abe K, Thung SN, Bodenheimer HC. Absence of hepatitis G virus within liver tissue of patients undergoing transplantation for cryptogenic cirrhosis. Transplantation 1999;67:1193-1197.
18. Zhao Q, Li FQ, Wu JG, Chu XM, Zhou Y, Yao XD. Negative correlation between cyclophilin mRNA level in leukocytes of renal allograft recipients and ciclosporin concentration in whole blood. Acta Pharm Sinica 1998;19:276-278.
19. Grude P, Boleslawski E, Conti F, Chouzenoux S, Calmus Y. MDR1 gene expression in peripheral blood mononuclear cells after liver transplantation. Transplantation 2002;73:1824-1828.
20. Dassi C, Signorini S, Gerthoux P, Cazzaniga M, Brambilla P. Cytochrome p450 1B1 mRNA measured in blood mononuclear cells by quantitative reverse transcription PCR. Clin Chem 1998;44:2416-2421.
21. Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res 1996;6:986-994.
22. Kruse N, Pette M, Toyka K, Rieckmann P. Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood. J Immunol Methods 1997;210:195-203.
23. Härtel C, Bein G, Kirchner H, Klüher H. A human whole blood assay for analysis of T-cell function by quantification of cytokine mRNA. Scand J Immunol 1999;49:649-654.
24. Kreuzer KA, Lass U, Landt O, et al. Highly selective and specific fluorescence reverse transcription-PCR assay for pseudogene-free detection of β-actin transcripts as quantitative reference. Clin Chem 1999;45:297-300.
25. Leccia JR, Milano G, Formento P, Francoual M, Formento JL, Pierrefite V. A clinically applicable assay for tumoral thymidylate synthase combining reverse transcriptase-PCR and high performance liquid chromatography. Eur J Cancer 1998;34:182-187.
26. Waha A, Watzka M, Koch A, et al. A rapid and sensitive protocol for competitive reverse transcriptase PCR analysis of cellular genes. Brain Pathol 1998;8:13-18.
27. Pallisgaard N, Clausen N, Schroder H, Hokland P. Rapid and sensitive minimal residual disease detection in acute leukaemia by quantitative real-time RT-PCR exemplified by t(12;21) TEL-AML1 fusion transcript. Genes Chromosomes Cancer 1999;26:355-365.
28. Moriyama N, Yamaguchi T, Takeuchi T, et al. Semiquantitative evaluation of alpha1A-adrenoceptor subtype mRNA in human hypertrophied and non-hypertrophied prostates: Regional comparison. Life Sci 1999;64:201-210.
29. Gipson IK, Spurr-Michaud S, Moccia R, et al. MUC4 and MUC5B transcripts are prevalent mucin messenger ribonucleic acids of the human endocervix. Biol Reprod 1999;60:58-64.
30. Morehouse CA, Owen RD. Exposure of Daudi cells to low-frequency magnetic fields does not elevate MYC steady-state mRNA levels. Radiat Res 2000;153:663-669.
31. Reddy NR, Wilkie BN. Quantitation of porcine cytokine and β2-microglobulin mRNA expression by reverse transcription polymerase chain reaction. J Immunol Methods 2000;233:83-93.
32. May WS Jr., Cuatrecasas P. Transferrin receptor: It's biological significance. J Membr Biol 1985;88:205-215.
33. Huebers HA, Finch CA. The physiology of transferrin and transferrin receptors. Physiol Rev 1987;67:520-582.
34. Pattanapanyasat K, Hoy TG. Expression of cell surface transferrin receptor and intracellular ferritin after in vitro stimulation of peripheral blood T lymphocytes. Eur J Haematol 1991;47:140-145.
35. Lipman ML, Shen Y, Jeffery JR, et al. Immune-activation gene expression in clinically stable renal allograft biopsies: Molecular evidence for subclinical rejection. Transplantation 1998;66:1673-1681.
36. Schweizer RT, Moore R, Bartus SA, Bow L, Hayden J. Beta 2-microglobulin monitoring after renal transplantation. Transplant Proc 1981;13:1620-3.
37. Goldman MR, Lippman R, Landwehr D, et al. Beta 2 microglobulin and the diagnosis of cardiac transplant rejection. Transplantation 1983;36:209-211.
38. Edwards LC, Helderman JH, Hamm LL, Ludwin D, Gailiunas Jr P, Hull AR. Noninvasive monitoring of renal transplant function by analysis of beta2-microglobulin. Kidney Int 1983;23:767-770.
39. Vernon WB, DeLong ER, Briner WH, Bollinger RR. Renal transplant monitoring with serum β-2-microglobulin and creatinine. Transplant Proc 1985;17:646-651.
40. Woodward JE, Bayer AL, Baliga P. Enhanced allograft survival via simultaneous blockade of transferrin receptor and interleukin-2 receptor. Transplantation 1999;68:1369-1376.
41. Harrison DC, Medhurst HD, Bond BC, Campbell CA, Davis RP, Philpott KL. The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischaemia. Molecular Brain Research 2000;75:143-149.
42. Weisinger G, Gavish M, Mazurika C, Zinder O. Transcription of β-actin, cyclophilin and GAPDH genes: Tissue- and treatment-specificity. Biochim Biophys Acta 1999;1446:225-232.
43. Zhong H, Simons JW. Direct comparison of GAPDH, β-actin, cyclophilin and 28s rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Comm 1999;259:523-526.
44. Foss DL, Baarsch MJ, Murtaugh MP. Regulation of HPRT, GAPDH and β-actin mRNA expression in porcine immune cells and tissues. Animal Biotech 1998;9:67-78.
45. Miyamoto S, Safer B. Immunosuppressants FK506 and rapamycin have different effects on the biosynthesis of cytoplasmic β-actin during the early period of T cell activation. Biochem J 1999;344:803-812.
46. Garcia-Monzon C, Majano PL, Solis JA, et al. Intrahepatic enhanced expression of beta2-microglobulin conformational epitope in acute liver allograft rejection: Evidence of modulation by glucocorticoids. Dig Dis Sci 1998;43:1755-1762.