Gap Junction-Mediated Intercellular Communication in a Long-Term Primary Mouse Hepatocyte Culture System

Hepatology

Volumn 38, Issue 5, 2003, Pages 1125-1135

  Stoehr, Stephanie A ^a   Isom, Harriet C ^a  

^a PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 26; CONNEXIN 32; DIMETHYL SULFOXIDE; GAP JUNCTION PROTEIN;

ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL DIFFERENTIATION; CELL FUNCTION; CELL ISOLATION; CELL TYPE; CELL ULTRASTRUCTURE; GAP JUNCTION; GENE DISRUPTION; GENE EXPRESSION REGULATION; GROWTH REGULATION; IMMUNOHISTOCHEMISTRY; LIVER CELL CULTURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0142151774     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1053/jhep.2003.50418     Document Type: Article

References (49)

1. Isom HC, Secott T, Georgoff I, Woodworth C, Mummaw J. Maintenance of differentiated rat hepatocytes in primary culture. Proc Natl Acad Sci U S A 1985;82:3252-3256.
2. Bilello JP, Delaney WE 4th, Boyce FM, Isom HC. Transient disruption of intercellular junctions enables baculovirus entry into nondividing hepatocytes. J Virol 2001;75:9857-9871.
3. Bilello JP, Cable EE, Myers RL, Isom HC. Role of paracellular junction complexes in baculovirus-mediated gene transfer to non-dividing rat hepatocytes. Gene Ther 2003;10:733-749.
4. Bour ES, Ward LK, Cornman GA, Isom HC. Tumor necrosis factor-alpha-induced apoptosis in hepatocytes in long-term culture. Am J Pathol 1996;148:485-495.
5. Cable EE, Connor JR, Isom HC. Accumulation of iron by primary rat hepatocytes in long-term culture: changes in nuclear shape mediated by non-transferrin-bound forms of iron. Am J Pathol 1998;152:781-792.
6. Cable EE, Isom HC. Exposure of primary rat hepatocytes in long-term DMSO culture to selected transition metals induces hepatocyte proliferation and formation of duct-like structures. HEPATOLOGY 1997;26:1444-1457.
7. Isom H, Georgoff I, Salditt-Georgieff M, Darnell JE Jr. Persistence of liver-specific messenger RNA in cultured hepatocytes: different regulatory events for different genes. J Cell Biol 1987;105:2877-2885.
8. Ruch RJ, Klaunig JE. Kinetics of phenobarbital inhibition of intercellular communication in mouse hepatocytes. Cancer Res 1988;48:2519-2523.
9. Wang H, Gao X, Fukumoto S, Tademoto S, Sato K, Hirai K. Differential expression and regulation of chemokines JE, KC, and IP-10 gene in primary cultured murine hepatocytes. J Cell Physiol 1999;181:361-370.
10. Kojima T, Fort A, Tao M, Yamamoto M, Spray DC. Gap junction expression and cell proliferation in differentiating cultures of Cx43 KO mouse hepatocytes. Am J Physiol 2001;281:G1004-G1013.
11. Na MR, Koo SK, Kim DY, Park SD, Rhee SK, Kang KW, Joe CO. In vitro inhibition of gap junctional intercellular communication by chemical carcinogens. Toxicology 1995;98:199-206.
12. Paul DL. Molecular cloning of cDNA for rat liver gap junction protein. J Cell Biol 1986;103:123-134.
13. Zhang JT, Nicholson BJ. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA. J Cell Biol 1989;109:3391-3401.
14. Ren P, Ruch RJ. Inhibition of gap junctional intercellular communication by barbiturates in long-term primary cultured rat hepatocytes is correlated with liver tumour promoting activity. Carcinogenesis 1996;17:2119-2124.
15. Zhang M, Thorgeirsson SS. Modulation of connexins during differentiation of oval cells into hepatocyres. Exp Cell Res 1994;213:37-42.
16. Kojima T, Yamamoto M, Tobioka H, Mizuguchi T, Mitaka T, Mochizuki Y. Changes in cellular distribution of connexins 32 and 26 during forma-rion of gap junctions in primary cultures of rat hepatocytes. Exp Cell Res 1996;223:314-326.
17. El-Fouly MH, Trosko JE, Chang CC. Scrape-loading and dye transfer. A rapid and simple technique to study gap junctional intercellular communication. Exp Cell Res 1987;168:422-430.
18. Nelles E, Butzler C, Jung D, Temme A, Gabriel HD, Dahl U, Traub O, et al. Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice. Proc Natl Acad Sci U S A 1996;93:9565-9570.
19. Temme A, Buchmann A, Gabriel HD, Nelles E, Schwarz M, Willecke K. High incidence of spontaneous and chemically induced liver tumors in mice deficient for connexin32. Curr Biol 1997;7:713-716.
20. Madden CR, Finegold M, Slagle BL. Expression of hepatitis B virus X protein does not alter the accumulation of spontaneous mutations in transgenic mice. J Virol 2000;74:5266-5272.
21. Elsdale T, Bard J. Collagen substrata for studies on cell behavior. J Cell Biol 1972;54:626-637.
22. Cable EE, Miller TG, Isom HC. Regulation of heme metabolism in rat hepatocytes and hepatocyte cell lines: delta-aminolevulinic acid synthase and heme oxygenase are regulated by different heme-dependent mechanisms. Arch Biochem Biophys 2000;384:280-295.
23. Bilello JP, Cable EE, Isom HC. Expression of E-cadherin and other paracellular junction genes is decreased in iron-loaded hepatocytes. Am J Pathol 2003;162:1323-1338.
24. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
25. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, et al. Measurement of protein using bicinchoninic acid. Anal Biochem 1985;150:76-85.
26. Laemmli UK. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 1970;227:680-685.
27. Kojima T, Mitaka T, Mizuguchi T, Mochizuki Y. Effects of oxygen radical scavengers on connexins 32 and 26 expression in primary cultures of adult rat hepatocytes. Carcinogenesis 1996;17:537-544.
28. Contreras B, Talamantes F. Growth hormone (GH) and 17beta-estradiol regulation of the expression of mouse GH receptor and GH-binding protein in cultured mouse hepatocytes. Endocrinology 1999;140:4725-4731.
29. Liu S, Gallo DJ, Green AM, Williams DL, Gong X, Shapiro RA, Gambotto AA, et al. Role of toll-like receptors in changes in gene expression and NF-kappa B activation in mouse hepatocytes stimulated with lipopolysaccharide. Infect Immun 2002;70:3433-3442.
30. Watanabe Y, Osaki H, Akaike T. TNF-alpha bifunctionally induces proliferation in primary hepatocytes: role of cell anchorage and spreading. J Immunol 1997;159:4840-4847.
31. Prowse KR, Baumann H. Interleukin-1 and interleukin-6 stimulate acutephase protein production in primary mouse hepatocytes. J Leukoc Biol 1989;45:55-61.
32. Kobusch AB, Bock KW. Zinc increases EGF-stimulated DNA synthesis in primary mouse hepatocytes. Studies in tumor promoter-treated cell cultures. Biochem Pharmacol 1990;39:555-558.
33. Qiao L, Leach K, McKinstry R, Gilfor D, Yacoub A, Park JS, Grant S, et al. Hepatitis B virus X protein increases expression of p21(Cip-1/WAF1/MDA6) and p27(Kip-1) in primary mouse hepatocytes, leading to reduced cell cycle progression. HEPATOLOGY 2001;34:906-917.
34. Schlosser SF, Azzaroli F, Dao T, Hingorani R, Nicholas Crispe I, Boyer JL. Induction of murine hepatocyte death by membrane-bound CD95 (Fas/APO-1)-ligand: characterization of an in vitro system. HEPATOLOGY 2000;32:779-785.
35. Smets FN, Chen Y, Wang LJ, Soriano HE. Loss of cell anchorage triggers apoptosis (anoikis) in primary mouse hepatocytes. Mol Genet Metab 2002;75:344-352.
36. Rahman S, Carlile G, Evans WH. Assembly of hepatic gap junctions. Topography and distribution of connexin32 in intracellular and plasma membranes determined using sequence-specific antibodies. J Biol Chem 1993;268:1260-1265.
37. George CH, Kendall JM, Evans WH. Inrracellular trafficking pathways in the assembly of connexins into gap junctions. J Biol Chem 1999;274:8678-8685.
38. Musil LS, Le AC, VanSlyke JK, Roberts LM. Regulation of connexin degradation as a mechanism to increase gap junction assembly and function. J Biol Chem 2000;275:25207-25215.
39. Bennett MV, Barrio LC, Bargiello TA, Spray DC. Gap junctions: new tools, new answers, new questions. Neuron 1991;6:305-320.
40. Lang L, Beyer EC, Schwartz AL, Gitlin JD. Molecular cloning of a rat uterine gap junction protein and analysis of gene expression during gestation. Am J Physiol 1991;260:E787-E793.
41. Loewenstein WR. Junctional intercellular communication and the control of growth. Biochim Biophys Acta 1979;560:1-65.
42. Mesnil M, Yamasaki H. Cell-cell communication and growth control of normal and cancer cells: evidence and hypothesis. Mol Carcinog 1993;7:14-17.
43. Charles AC, Naus C, Zhu D, Kidder G, Dirksen E, Sanderson M. Intercellular calcium signaling via gap junctions in glioma cells. J Cell Biol 1992;118:195-201.
44. Young JDE, Cohn ZA, Gilula NB. Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kDa protein forms a junctional channel. Cell 1987;48:733-743.
45. Fletcher WH, Greenan JRH. Receptor-mediated action without receptor occupancy. Endocrinology 1985;116:1660-1662.
46. Trosko JE, Chang CC. Role of intercellular communication in tumor promotion. Mech Tumor Prom 1984;4:119-145.
47. Kalimi GH, Sirsat SM. Phorbol ester tumor promoter affects the mouse epidermal gap junctions. Cancer Lett 1984;22:211-258.
48. Kalimi GH, Sirsat SM. The relevance of gap junctions to stage I tumor promotion in mouse epidermis. Carcinogenesis 1984;5:1671-1677.
49. Sugie S, Mori H, Takahashi M. Effect of in vivo exposure to the liver tumor promoters phenobarbital or DDT on the gap junctions of rat hepatocytes: a quantitative freeze-fracture analysis. Carcinogenesis 1987;8:45-51.