Chapter 10 Lipid Membrane and Ligand-Gated Ion Channels in General Anesthetic Action

Current Topics in Membranes and Transport

Volumn 48, Issue C, 1999, Pages 311-350

  Sandberg, Warren S ^a   Dubois, Brian W ^a   Miller, Keith W ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0142102123     PISSN: 00702161     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0070-2161(08)61048-5     Document Type: Article

References (131)

1. Addona G., Sanderman H., Kloczewiak M., Husain S., and Miller K. Where does cholesterol act during activation of the nicotinic acetylcholine receptor?. Biochim. Biophys. Acta 1370 (1998) 299-309
2. Akaike N., Hattori K., Inomata N., and Oomura Y. γ-Aminobutyric-acid- and pentobarbitone-gated chloride currents in internally perfused frog sensory neurones. J. Physiol. (London) 360 (1985) 367-386
3. Akeson M., and Deamer D. Steady-state catecholamine distribution in chromaffin granule preparations: A test of the pump-leak hypothesis of general anesthesia. Biochemistry 28 (1989) 5120-5127
4. Alifimoff J., Firestone L., and Miller K. Anesthetic potencies of secondary alcohol enantiomers. Anesthesiology 66 (1987) 55-59
5. Alifimoff J., Firestone L., and Miller K. Anesthetic potencies of primary alkanols: Implications for the molecular dimensions of the anesthetic site. Br. J. Pharmacol. 96 (1989) 9-16
6. Antollini S., Soto M., Bonini de Romanelli I., Gutierrez-Merino C., Sotomayor P., and Barrantes F. Physical state of bulk and protein-associated lipid in nicotinic acetylcholine receptor-rich membrane studied by Laurdan generalized polarization and fluorescence energy transfer. Biophys. J. 70 (1996) 1275-1284
7. Ashton D., and Wauquier A. Modulation of a GABA-ergic inhibitory circuit in the in vitro hippocampus by etomidate isomers. Anesth. Analg. (N.Y.) 64 (1985) 975-980
8. 1H NOE spectroscopy. Biochemistry 34 (1995) 6533-6539
9. Bangham A., and Mason W. Anesthetics may act by collapsing pH gradients. Anesthesiology 53 (1980) 135-141
10. Bangham A., Standish M., and Miller N. Cation permeability of phospolipid model membranes: Effect of narcotics. Nature (London) 208 (1965) 1295-1297
11. Belelli D., Lambert J.J., Peters J.A., Wafford K., and Whiting P.J. The interaction of the general anesthetic etomidate with the γ-aminobutyric acid type A receptor is influenced by a single amino acid. Proc. Nat. Acad. Sci. U.S.A. 94 (1997) 11031-11036
12. Bennett P., and Simmonds M. The influence of membrane cholesterol on the GABAA receptor. Brit. J. Pharmacol. 117 (1996) 87-92
13. Bernard C. Lecons sur les Anesthetiques et sur I'Asphyxie. (1875), Bailliere, Paris
14. Boggs J., Yoong T., and Hsia J. Site and mechanism of anesthetic action: I. Effect of anesthetics and pressure on fluidity of spin-labeled lipid vesicles. Mol. Pharmacol. 12 (1976) 127-135
15. Bradley D., and Richards C. Temperature dependence of the action of nerve blocking agents and its relationship to membrane-buffer partition coefficients: Thermodynamic implications for the site of action of local anesthetics. Br. J. Pharmacol. 81 (1984) 161-167
16. Bradley R., Sterz R., and Peper K. The effects of alcohols and diols at the nicotinic acetylcholine receptor of the neuromuscular junction. Brain Res. 295 (1984) 101-112
17. Browning M., Bureau M., Dudek E., and Olsen R. Protein kinase C and cAMPdepen-dent protein kinase phosphorylate the β subunit of the purified γ-aminobutyric acid A receptor. Proc. Nat. Acad. Sci. U.S.A. 87 (1990) 1315-1318
18. Bull M., Brailsford J., and Bull B. Erythrocyte membrane expansion due to the volatile anesthetics, the 1-alkanols, and benzyl alcohol. Anesthesiology 57 (1982) 399-403
19. Cafiso D. Influence of charges and dipoles on macromolecular adsorption and permeability. In: Disalvo E., and Simon S. (Eds). Permeability and Stability of Lipid Bilayers. (1995), CRC Press, Boca Raton, Florida 179-195
20. Cantor R. The lateral pressure profile in membranes: A physical mechanism of general anesthesia. Biochemistry 36 (1997) 2339-2344
21. Changeux J.-P., Galzi J.-L., DevillerS-Thiery A., and Bertrand D. The functional architecture of the acetylcholine nicotinic receptor explored by affinity labelling and sitedirected mutagenesis. Q. Rev. Biophys. 25 (1992) 395-432
22. Chothia C. The nature of the accessible and buried surfaces in proteins. J. Mol. Biol. 105 (1976) 1-14
23. Colley C., and Metcalfe J. The localisation of small molecules in lipid bilayers. FEBS Lett. 24 (1972) 241-246
24. A and glutamate receptors: Relation to in vivo effects. Br. J. Pharmacol. 118 (1996) 378-384
25. Dilger J., and Vidal A. Cooperative interactions between general anesthetics and QX-222 within the pore of the acetylcholine receptor ion channel. Mol. Pharmacol. 45 (1994) 169-175
26. Dodson B., Braswell L., and Miller K. Barbiturates bind to an allosteric regulatory site on nicotinic acetylcholine receptor-rich membranes. Mol. Pharmacol. 32 (1987) 119-126
27. Dreger M., Krauss M., Herrmann A., and Hucho F. Interactions of the nicotinic acetylcholine receptor transmembrane segments with the lipid bilayer in native receptorrich membranes. Biochemistry 36 (1997) 839-847
28. Dwyer R., Bennett H., Eger II E., and Heilbron D. Effects of isoflurane and nitrous oxide in subanesthetic concentrations on memory and responsiveness in volunteers. Anesthesiology 77 (1992) 888-898
29. Eger II E., Saidman L., and Brandstater B. Temperature dependence of halothane and cyclopropane anesthesia in dogs: Correlation with some theories of anesthetic action. Anesthesiology 26 (1965) 764-770
30. Eger II E.I., Koblin D.D., Laster M.J., Schurig V., Juza M., Ionescu P., and Gong D. Minimum alveolar aqesthetic concentration values for the enantiomers of isoflurane differ minimally. Anesrh. Analg. (N.Y.) 85 (1997) 188-192
31. Eger II E., Koblin D., Harris R., Kendig J., Pohorille A., Halsey M., and Trudell J. Hypothesis: Inhaled aneqthetics produce immobility and amnesia by different mechanisms at different sites. Anesth. Analg. (N.Y.) 84 (1997) 915-918
32. Firestone L.L., Miller J.C., and Miller K.W. Tables of physical and pharmacological properties of anesthetics. In: Roth S.H., and Miller K.W. (Eds). Molecular and Cellular Mechanisms of Anesthetics (1986), Plenum, New York 455-470
33. Firestone S., Ferguson C., and Firestone L. Isoflurane's optical isomers are equipotent in Rana pipiens tadpoles. Anesthesiology 77 (1992) A758
34. Firestone S., Firestone L., Ferguson C., and Blanck D. Staurosporine, a protein kinase C inhibitor, decreases the general anesthetic requirement in Rana pipiens tadpoles. Anesrh. Analg. (N.Y.) 77 (1993) 1026-1030
35. Fong T., and McNamee M. Correlation between acetylcholine receptor function and structural properties of membranes. Biochemistry 25 (1986) 830-840
36. Forman S.A. Direct interactions of anesthetics and nonanesthetics with the nicotinic acetylcholine receptor pore. Toxicol. Lett. 100 (1998) 169-178
37. Forman S.A., Miller K., and Yellen G. A discrete site for general anesthetics on a postsynaptic receptor. Mol. Pharmacol. 48 (1995) 547-581
38. Franks N., and Lieb W. Where do general anesthetics act?. Nature (London) 274 (1978) 339-342
39. Franks N., and Lieb W. Do general anaesthetics act by competitive binding to specific receptors?. Nature (London) 310 (1984) 599-601
40. Franks N., and Lieb W. Partitioning of long-chain alcohols into lipid bilayers: Implications for mechanisms of general anesthesia. Proc. Nat. Acad. Sci. U.S.A. 83 (1986) 5116-5120
41. Franks N., and Lieb W. Stereospecific effects of inhalational general anesthetic optical isomers on nerve ion channels. Science 254 (1991) 427-430
42. Goodman D., Nemoto E., Evans R., and Winter P. Anesthetics modulate phospholipase C hydrolysis of monolayer phospholipids by surface pressure. Chem. Phys. of Lipids 84 (1996) 57-64
43. Gruner S., and Shyamsunder E. Is the mechanism of general anesthesia related to lipid membrane spontaneous curvature?. Ann. N. Y. Acad. Sci. 625 (1991) 685-697
44. Harris B.D., Moody E.J., Basile A.S., and Skolnick P. Volatile anesthetics bidirectionally and stereospecifically modulate ligand binding to GABA receptors. Eur. J. Pharmacol. 267 (1994) 269-274
45. Harris B., Wong G., Moody E., and Skolnick P. Different subunit requirements for volatile and nonvolatile anesthetics at γ-aminobutyric acid type A receptors. Mol. Pharmacol. 47 (1995) 363-367
46. Harrison N., Kugler J., Jones M., Greenblatt E., and Pritchett D. Positive modulation of human γ-aminobutyric acid type A and glycine receptors by the inhalation anesthetic isoflurane. Mol. Pharmacol. 44 (1993) 628-632
47. Harvey E. Effect of certain organic and inorganic substances upon light production by luminous bacteria. Biol. Bull. 29 (1915) 308-311
48. Huang L.-Y.M., and Barker J.L. Pentobarbital: Stereospecific actions of (+) and (-) isomers revealed on cultured mammalian neurons. Science 207 (1980) 195-197
49. Jacobs R., and White S. Behavior of hexane dissolved in dimyristoylphosphatidylcholine bilayers: An NMR and calorimetric study. J. Am. Chem. Soc. 106 (1984) 915-920
50. Jerusalinsky D., Quillfeldt J., Walz R., Da Silva R., Medina J., and Izquierdo I. Post-training intrahippocampal infusion of protein kiase C inhibitors causes amnesia in rats. Behav. Neural Biol. 61 (1994) 107-109
51. Johnson F., and Flagler E. Hydrostatic pressure reversal of narcosis in tadpoles. Science 112 (1950) 91-92
52. Johnson S., and Bangham A. The action of anesthetics on phospolipid membranes. Biochim. Biophys. Acta 193 (1969) 92-104
53. Johnson S., Miller K., and Bangham A. The opposing effects of pressure and general anesthetics on the cation permeability of liposomes of varying lipid composition. Biochim. Biophys. Acta 307 (1973) 42-57
54. c receptors: Relatively simple transmitter gated ion channels?. Trends Pharmacol. Sci. 17 (1996) 319-323
55. Jones O., and McNamee M. Annular and nonannular binding sites for cholesterol associated with the nicotinic acetylcholine receptor. Biochemistry 27 (1988) 2364-2374
56. Kamaya H., Ueda I., Moore P., and Eyring H. Antagonism between high pressure and anesthetics in the thermal phpse-transition of dipalmitoyl phosphatidylcholine bilayer. Biochim. Biophys. Acta 550 (1979) 131-137
57. Kandel L., Chortkoff B., Sonner J., Laster M., and Eger II E. Nonanesthetics can suppress learning. Anesth. Analg. (N.Y.) 82 (1996) 321-326
58. Kita Y., Bennett L., and Miller K. The partial molar volumes of anesthetics in lipid bilayers. Biochim. Biophys. Acta 647 (1981) 130-139
59. Koblin D., Chortkoff B., Laster M., Eger II E., Halsey M., and Ionescu P. Polyhalogenated and perfluorinated compounds that disobey the Meyer-Overton hypothesis. Anesrh. Analg. (N.Y.) 79 (1994) 1043-1048
60. LaBella F., and Queen G. General anesthetics inhibit cytochrome P450 monooxygenases and arachidonic acid metabolism. Can. J. Physiol. Pharmacol. 71 (1993) 48-53
61. LaBella F., Chen Q.-M., Stein D., and Queen G. The site of general anaesthesia and cytochrome P450 oxygenases: Similarities defined by straight chain and cyclic alcohols. Br. J. Pharmacol. 120 (1997) 1158-1164
62. Landau E., and Rosenbusch J. Lipidic cubic phases: A novel concept for the crystallization of membrane proteins. Proc. Nat. Acad. Sci. U.S.A. 93 (1996) 14532-14535
63. 1-tetrahydrocannabinol and other cannabinoids on spin-labeled liposomes and their relationship to mechanisms of general anesthesia. Mol. Pharmacol. 11 (1975) 595-602
64. Leonard R., Charnet P., Labarca C., Vogelaar N., Czyzyk L., Gouin A., Davidson N., and Lester H. Reverse pharmacology of the nicotinic acetylcholine receptor: Mapping the local anesthetic binding site. Ann. N. Y. Acad. Sci. 625 (1991) 588-599
65. Lever M., Miller K., Paton W., and Smith E. Pressure reversal of anaesthesia. Nature (London) 231 (1971) 368-371
66. Lieb W., Kovalycsik M., and Mendelsohn R. Do clinical levels of general anaesthetics affect lipid bilayers? Evidence from Raman scattering. Biochim. Biophys. Acta 688 (1982) 388-398
67. Lingle C.J., Maconochie D., and Steinbach J.H. Activation of skeletal muscle nicotinic acetylcholine receptors, [Review]. J. Membr. Biol. 126 (1992) 195-217
68. Liu J., Laster M., Taheri S., Eger II E., Koblin D., and Halsey M. Is there a cutoff in anesthetic potency for the normal alkanes?. Anesrh. Analg. (N.Y.) (1993) 12-18
69. Liu J., Laster M., Koblin D., Eger II E., Halsey M., Tahere S., and Chortkof B. A cutoff in potency exists in the perfluoroalkanes. Anesth. Analg. (N.Y.) 79 (1994) 238-244
70. Lundbaek J., Maer A., and Anderson O. Lipid bilayer electrostatic energy, curvature stress, and the assembly of gramicidin channels. Biochemistry 36 (1997) 5695-5701
71. Lysko G., Robinson J., Casto R., and Ferrone R. The stereopecific effects of isoflurane isomers in vivo. Eur. J. Pharmacol. 263 (1994) 25-29
72. γ. Science 278 (1997) 279-283
73. Marsh D., and Barrantes F. Immobilized lipid in acetylcholine receptor-rich membranes from Torpedo marmorata. Proc. Nat. Acad. Sci. U.S.A. 75 (1978) 4329-4333
74. Mattevi A., Rizzi M., and Bolognesi M. New structures of allosteric proteins revealing remarkable conformational changes. [Review] [35 refs.]. Curr. Opin. Struct. Biol. 6 (1996) 824-829
75. Melchior D., Scavitto F., and Steim J. Dilatometry of diplamitoyllecithin-cholesterol bilayers. Biochemistry 19 (1980) 4828-4834
76. Metcalfe J., Seeman P., and Burgen A. The proton relaxation of benzyl alcohol in erythrocyte membranes. Mol. Pharmacol. 4 (1968) 87-95
77. Meyer H.H. Zur Theorie der Alkoholnarkose. Der Einfluss wechselnder Temperatur auf Wirkungsstärke und Theilungscoefficient der Narcotica. Arch. Exp. Pathol. Pharmakol. 42 (1901) 338-346
78. Mihic S., McQuilkin S., Eger II E., Ionescu P., and Harris R. Potentiation of γ-aminobutyric acid type A receptor mediated chloride currents by novel halogenated compounds correlates with their abilities to induce general anesthesia. Mol. Pharmacol. 46 (1994) 851-857
79. Mihic S., Ye Q., Wick M., Koltchine V., Krasowski M., Finn S., Mascia M., Valenzuela C., Hanson K., Greenblatt E., Harris R., and Harrison N. Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature, (London) 389 (1997) 385-389
80. Miller K., Paton W., Smith R., and Smith E. The pressure reversal of general anesthesia and the critical volume hypothesis. Mol. Pharmacol. 9 (1973) 131-143
81. Miller K., Sauter J., and Braswell L. A stereoselective pentobarbital binding site in cholinergic membranes from Torpedo californica. Biochem. Biophys. Rex Commun. 105 (1982) 659-666
82. Miller K., Firestone L., Alifimoff J., and Streicher P. Nonanesthetic alcohols dissolve in synaptic membranes without perturbing their lipids. Proc. Nat. Acad. Sci. U3.A 86 (1989) 1084-1087
83. Miller K., Addona G., and Kloczewiak M. Approaches to proving there are general anesthetic sites of action on ligand gated channels. Toxicol. Lett. 100 (1998) 139-147
84. A receptor complex. Brain Res. 615 (1993) 101-106
85. A receptors. J. Neurochem. 69 (1997) 1310-1313
86. Moss G., Curry S., Franks N., and Lieb W. Mapping the polarity profiles of general anesthetic target sites using n-alkane-(α,)-diols. Biochemistry 30 (1991) 10551-10557
87. Mountcastle D., Biltonen R., and Halsey M. Effect of anesthetics and pressure on the thermotropic behavior of multilamellar dipalmitoylphosphatidylcholine liposomes. Proc. Nat. Acad. Sci. U.S.A. 75 (1978) 4906-4910
88. Nakahiro M., Arakawa O., and Narahashi T. Modulation of γ-aminobutyric acid receptor-channel complex by alcohols. J. Pharmacol. Exp. Ther. 259 (1991) 235-240
89. +2-ATPase of sarcoplasmic reticulum. Biochemistry 23 (1984) 130-135
90. A receptor determined by eloctron microscopic image analysis. J. Neurochem. 62 (1994) 815-818
91. North C., and Cafiso D.S. Contrasting membrane localization and behavior of halogenated cyclobutanes that follow or violate the Meyer-Overton hypothesis of general anesthetic potency. Biophys. J. 72 (1997) 1754-1761
92. Olsen R.W., Fischer J.B., and Dunwiddie T.V. Barbiturate enhancement of γ-aminobutyric acid receptor binding and function as a mechanism of anesthesia. In: Roth S.H., and Miller K.W. (Eds). Molecular and Cellular Mechanisms of Anesthetics (1986), Plenum, New York 165-178
93. Overton C.E. Studies of narcosis and a contribution to general pharmacology. In: Lipnick R.L. (Ed). Studies of Narcosis (1991), Chapman and Hall and the Wood Library-Museum of Anesthesiology, London 1-203
94. Pang K.-Y., Braswell L., Chang L., Sommer T., and Miller K. The perturbation of lipid bilayers by general anesthetics: A quantitative test of the disordered lipid hypothesis. Mol. Pharmacol. 18 (1980) 84-90
95. Parker I., Gundersen C., and Miledi R. Actions of pentobarbital on rat brain receptors expressed in Xenopus oocytes. J. Neurosci. 6 (1986) 2290-2297
96. Pope J., Walker L., and Dubro D. On the ordering of N-alkane and N-alcohol solutes in phospholipid bilayer model membrane systems. Chem. Phys. Lipids 35 (1984) 259-277
97. Pringle M., Brown K., and Miller K. Can the lipid theories of anesthesia account for the cutoff in anesthetic potency in homologous series of alcohols?. Mol. Pharmacol. 19 (1981) 49-55
98. Qin Z., Szabo G., and Cafiso D. Anesthetics reduce the magnitude of the membrane dipole potential. Measurements in lipid vesicles using voltage sensitive spin probes. Biochemistry 34 (1995) 5536-5543
99. Quinlan J., Homanics G., and Firestone L. Anesthesia sensitivity in mice that lack the β3 subunit of the γ-aminobutyric acid type A receptor. Anesthesiology 88 (1998) 775-780
100. Raines D. Anesthetic and nonanesthetic halogenated volatile compounds have dissimilar activities on nicotinic acetycholine receptor desensitization kinetics. Anesthesiology 84 (1996) 663-671
101. Raines D.E., and Cafiso D.S. The enhancement of proton/hydroxyl flow across lipid vesicles by inhalation anesthetics. Anesthesiology 70 (1989) 57-63
102. Raines D., and Miller K. The role of charge in lipid selectivity for the nicotinic acetylcholine receptor. Biophys. J. 64 (1993) 632-641
103. Raines D.E., Korten S.E., Hill A.G., and Miller K.W. Anesthetic cutoff in cycloalkanemethanols. A test of current theories. Anesthesiology 78 (1993) 918-927
104. Rand R., and Pangborn W. A structural transition in egg lecithin-cholesterol bilayers at 12°C. Biochim. Biophys. Acta 318 (1973) 299-305
105. Rankin S., Raines D., and Miller K. Isoflurane's actions on the nicotinic acetylcholine receptor are dependent upon the Composition of the lipid bilayer. Anesthesiology 81 (1994) A884
106. Rankin S., Addona G., Kloczewiak M., Bugge B., and Miller K. The cholesterol dependence of activation and fast desensitization of the nicotinic acetylcholine receptor. Biophys. J. 73 (1997) 2446-2455
107. Reyes J., and Latorre R. Effect of the anesthetics benzyl alcohol and chloroform on bilayers made from monolayers. Biophys. J. 28 (1979) 259-280
108. Richards F.M. Areas, volumes, packing, and protein structure. Annu. Rev. Biophys. Bioeng. 6 (1977) 151-176
109. Roth S.H., Forman S.A., Braswell L.M., and Miller K.W. Actions of pentobarbital enantiomers on nicotinic cholinesgic receptors. Mol. Pharmacol. 36 (1989) 874-880
110. Seeman P. Temperature dependence of erythrocyte membrane expansion by alcohol anesthetics. Possible support for the partition theory of anesthesia. Biochim. Biophys. Acta 183 (1969) 520-529
111. Shapira R., Silberberg S., Ginsburg S., and Rahamimoff R. Activation of protein kinase C augments evoked transmitter release. Nature, (London) 325 (1987) 58-60
112. A receptors and channel function. Neuron 5 (1990) 703-711
113. Slater S., Cox K., Lombardi J., Ho C., Kelly M., Rubin E., and Stubbs C. Inhibition of protein kinase C by alcohols and anaesthetics. Nature, (London) 364 (1993) 82-84
114. Slater S., Kelly M., Taddeo F., Ho C., Rubin E., and Stubbs C. The modulation of protein kinase C activity by membrane lipid bilayer structure. J. Biol. Chem. 269 (1994) 4866-4871
115. Slater S., Kelly M., Larkin J., Ho G., Mazurek A., Taddeo F., Yeager M., and Stubbs C. Interaction of alcohols and anesthetics with protein kinase C α. J. Biol. Chem. 272 (1997) 6167-6173
116. Steffey E., and Eger II E. Hyperthermia and halothane MAC in the dog. Anesthesiology 41 (1974) 392-396
117. Sunshine C., and McNamee M. Lipid modulation of nicotinic acetylcholine receptor function: The role of neutral and negatively charged lipids. Biochim. Biophys. Acta 1108 (1992) 240-246
118. Tang P., Yan B., and Xu Y. Different distribution of fluorinated anesthetics and nonanesthetics in model membrane: A 19F NMR study. Biophys. J. 72 (1997) 1676-1682
119. Tomlin S., Jenkins A., Lied W., and Franks N. Stereoselective effects of etomidate optical isomers on γ-aminobutyric acid type A receptors and animals. Anesthesiology 88 (1998) 708-717
120. Turner G., and Oldfield E. Effect of a local anesthetic on hydrocarbon chain order in membranes. Nature (London) 277 (1979) 669-670
121. Ueda I., Shieh D., and Eyring H. Anesthetic interaction with a model cell membrane: Expansion, phase transition, and melting of the lecithin monolayer. Anesthesiology 41 (1974) 217-225
122. Unwin N. Acetylcholine receptor channel imaged in the open state. Nature (London) 373 (1995) 37-43
123. + channel required for modulation by protein kinase C. Science 254 (1991) 866-868
124. White B., and Cohen J. Agonist-induced changes in the structure of the acetylcholine receptor M2 regions revealed by photoincorporation of an uncharged nicotinic noncompetitive antagonist. J. Biol. Chem. 267 (1992) 15770-15783
125. Wood S., Forman S., and Miller K. Short chain and long chain alkanols have different sites of action on nicotinic acetylcholine receptor channels from Torpedo. Mol. Pharmacol. 39 (1991) 332-338
126. Wood S., Hill W., and Miller K. Cycloalkanemethanols discriminate between volume and length-dependent loss of activity of alcohols at the Torpedo nicotinic acetylcholine receptor. Mol. Pharmacol. 44 (1993) 1219-1226
127. Wood S., Tonner P., de Armendi A., Bugge B., and Miller K. Channel inhibition by alkanols occurs at a binding site on the nicotinic acetylcholine receptor. Mol. Pharamacol. 47 (1995) 121-130
128. Wu G., Tonner P., and Miller K. Ethanol stabilizes the open channel state of the Torpedo nicotinic acetylcholine receptor. Mol. Pharmacol. 45 (1993) 102-108
129. 1, subunit. J. Gen. Physiol. 107 (1996) 195-205
130. Yeh J., Quandt F., Tanguy J., Nakahiro M., Narahashi T., and Brunner E. General anesthetic action on γ-aminobutyric acid-activated channels. Ann. N. Y. Acad. of Sci. 625 (1991) 155-173
131. Zhang G., Kazanietz M., Blumberg P., and Hurley J. Crystal structure of the cys2 activator-binding domain of protein kinase C gamma in complex with phorbol ester. Cell (Cambridge, Mass.) 81 (1995) 917-924