Construction and Preliminary Evaluation of an Aspergillus flavus Reporter Gene Construct as a Potential Tool for Screening Aflatoxin Resistance

Journal of Food Protection

Volumn 66, Issue 10, 2003, Pages 1927-1931

  Brown, Robert L ^a   Brown Jenco, Carmen S ^b   Bhatnagar, Deepak ^a   Payne, Gary A ^b  

^a SOUTHERN REGIONAL RESEARCH CENTER   (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPERGILLUS; ASPERGILLUS FLAVUS; ESCHERICHIA COLI; EUKARYOTA; FUNGI; ZEA MAYS;

EID: 0142095143     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-66.10.1927     Document Type: Article

References (35)

1. Brown, R. L., D. Bhatnagar, T. E. Cleveland, and J. W. Cary. 1998. Recent advances in preharvest prevention of mycotoxin contamination. p. 351-379. In K. K. Sinha and D. Bhatnagar (ed.), Mycotoxins in agriculture and food safety. Marcel Dekker, New York.
2. Brown, R. L., Z.-Y. Chen, T. E. Cleveland, and J. S. Russin. 1999. Advances in the development of host resistance in corn to aflatoxin contamination by Aspergillus flavus - a mini-review. Phytopathology 89:113-117.
3. Brown, R. L., T. E. Cleveland, G. A. Payne, C. P. Woloshuk, K. W. Campbell, and D. G. White. 1995. Determination of resistance to aflatoxin production in maize kernels and detection of fungal colonization using an Aspergillus flavus transformant expressing Escherichia coli β -glucuronidase. Phytopathology 85:983-989.
4. Brown, R. L., T. E. Cleveland, G. A. Payne, C. P. Woloshuk, and D. G. White. 1997. Growth of an Aspergillus flavus transformant expressing Escherichia coli beta-glucuronidase in maize kernels resistant to aflatoxin production. J. Food Prot. 60:84-87.
5. Brown, R. L., P. J. Cotty, T. E. Cleveland, and N. W. Widstrom. 1993. Living maize embryo influences accumulation of aflatoxin in maize kernels. J. Food Prot. 56:967-971.
6. Campbell, K. W., and D. G. White. 1995. Evaluation of corn genotypes for resistance to Aspergillus ear rot, kernel infection, and aflatoxin production. Plant Dis. 79:1039-1045.
7. Cary, J. W., M. Wright, D. Bhatnagar, R. Lee, and F. S. Chu. 1996. Molecular characterization of an Aspergillus parasiticus dehydrogenase gene, NorA, located on the aflatoxin biosynthesis gene cluster. Appl. Environ. Microbiol. 62:360-366.
8. Chang, P.-K., J. W. Cary, D. Bhatnagar, et al. 1993. Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Appl. Environ. Microbiol. 59:3273-3279.
9. 1 biosynthesis. Mol. Gen. Genet. 248:270-277.
10. Cotty, P. J. 1994. Influence of field application of an atoxigenic strain of Aspergillus flavus on the population of A. flavus infecting cotton bolls and on the aflatoxin content of cottonseed. Phytopathology 84:1270-1277.
11. Council for Agricultural Science and Technology. 1979. Aflatoxins and other mycotoxins: an agricultural perspective. Report no. 80. Council for Agricultural Science and Technology, Ames, Iowa.
12. Davis, N. D., S. K. Iyer, and U. L. Diener. 1987. Improved method of screening for aflatoxin with coconut agar medium. Appl. Environ. Microbiol. 53:1593-1595.
13. Dickens, J. W. 1987. Sampling and detection techniques for aflatoxin in maize, p. 92-99. In M. S. Zuber, E. B. Lillehoj, and B. L. Renfro (ed.), Aflatoxin in maize: a proceedings of the workshop. Centro Internacional de Mejoramiento de Maiz y Trigo, Mexico, D.F., Mexico.
14. Diener, U. L., R. L. Cole, T. H. Sanders, G. A. Payne, L. S. Lee, and M. A. Klich. 1987. Epidemiology of aflatoxin formation by Aspergillus flavus. Annu. Rev. Phytopathol. 25:249-270.
15. Feng, G. H., and T. J. Leonard. 1995. Characterization of the polyketide synthase gene (pksL1) required for aflatoxin biosynthesis in Aspergillus parasiticus. J. Bacteriol. 177:6246-6254.
16. Flaherty, J. E., M. A. Weaver, G. A. Payne, and C. P. Woloshuk. 1995. A beta-glucuronidase reporter gene construct for monitoring aflatoxin biosynthesis in Aspergillus flavus. Appl. Environ. Microbiol. 61:2482-2486.
17. Jefferson, R. A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Mol. Biol. Rep. 5:387-405.
18. Mahanti, N., D. Bhatnagar, J. W. Cary, I. Joubran, and J. E. Linz. 1996. Structure and function of fas-1A, a gene encoding a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl. Environ. Microbiol. 62:191-195.
19. Marsh, P. B., M. E. Simpson, R. J. Ferretti, et al. 1969. Mechanism of formation of a fluorescence in cotton fiber associated with aflatoxin in the seeds at harvest. Agric. Food Chem. 17:468-472.
20. Meyers, D. M., G. O'Brian, W. L. Du, D. Bhatnagar, and G. A. Payne. 1998. Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin. Appl. Environ. Microbiol. 64:3713-3717.
21. Papa, K. E. 1984. Genetics of Aspergillus flavus: linkage of aflatoxin mutants. Can. J. Microbiol. 30:68-73.
22. Payne, G. A., G. J. Nystrom, D. Bhatnagar, T. E. Cleveland, and C. P. Woloshuk. 1993. Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. Appl. Environ. Microbiol. 59:156-162.
23. Prieto, R., G. L. Yousibova, and C. P. Woloshuk. 1996. Identification of aflatoxin biosynthesis genes by genetic complementation in an Aspergillus flavus mutant lacking the aflatoxin gene cluster. Appl. Environ. Microbiol. 62:3567-3571.
24. 1 in peanut butter. J. Food Sci. 49:792-795.
25. 1 biosynthetic cluster. J. Biol. Chem. 271:13600-13608.
26. Skory, C. D., P.-K. Chang, J. Cary, and J. E. Linz. 1992. Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis. Appl. Environ. Microbiol. 58:3527-3537.
27. Trail, F., P.-K. Chang, J. Cary, and J. E. Linz. 1994. Structural and functional analysis of the nor-1 gene involved in the biosynthesis of aflatoxins by Aspergillus parasiticus. Appl. Environ. Microbiol 60:4078-4085.
28. Woloshuk, C. P., K. R. Foutz, J. F. Brewer, D. Bhatnagar, T. E. Cleveland, and G. A. Payne. 1994. Molecular characterization of aflR, a regulatory locus for aflatoxin biosynthesis. Appl. Environ. Microbiol. 60:2408-2414.
29. Woloshuk, C. P., and G. A. Payne. 1994. The alcohol dehydrogenase gene adh1 is induced in Aspergillus flavus grown on medium conducive to aflatoxin biosynthesis. Appl. Environ. Microbiol. 60:670-676.
30. Woloshuk, C. P., E. R. Seip, G. A. Payne, and C. R. Adkins. 1989. Genetic transformation system for the aflatoxin-producing fungus Aspergillus flavus. Appl. Environ. Microbiol. 55:86-90.
31. Yu, J., J. W. Cary, D. Bhatnagar, T. E. Cleveland, N. P. Keller, and F. S. Chu. 1993. Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an o-methyltransferase involved in aflatoxin biosynthesis. Appl. Environ. Microbiol. 59:3564-3571.
32. Yu, J., P.-K. Chang, J. W. Cary, D. Bhatnagar, and T. E. Cleveland. 1997. avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus. Appl. Environ. Microbiol. 63:1349-1356.
33. 2. Appl. Environ. Microbiol. 64:4834-4841.
34. Yu, J., P.-K. Chang, G. A. Payne, J. W. Cary, D. Bhatnagar, and T. E. Cleveland. 1995. Comparison of the omt A genes encoding o-methyltransferases involved in aflatoxin biosynthesis from Aspergillus parasiticus and A. flavus. Gene 63:121-125.
35. Yu, J., C. P. Woloshuk, D. Bhatnagar, and T. E. Cleveland. 2000. Cloning and characterization of avfA and omtB genes involved in aflatoxin biosynthesis in three Aspergillus species. Gene 248:157-167.