Rapid site-directed mutagenesis of chemokines and their purification from a bacterial expression system

Journal of Immunological Methods

Volumn 279, Issue 1-2, 2003, Pages 233-249

  Wain, Julie H ^a   Palmer, Jeremy M ^b   Kirby, John A ^a   Ali, Simi ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Chemokine; Cloning; Mutation; Polymerase chain reaction; Protein purification

Indexed keywords

CHEMOKINE; HEPARAN SULFATE; MONOCYTE CHEMOTACTIC PROTEIN 3;

ARTICLE; BINDING SITE; CELL MIGRATION; CHEMOTAXIS; CONTROLLED STUDY; DNA SCREENING; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; HUMAN CELL; INVERSE POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS);

EID: 0142061072     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(03)00252-7     Document Type: Article

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