A multiplex nested-PCR assay for sensitive and simultaneous detection and direct identification of phytoplasma in the Elm yellows group and Stolbur group and its use in survey of grapevine yellows in France

Vitis

Volumn 42, Issue 3, 2003, Pages 151-157

  Clair, D ^a   Larrue, J ^a   Albert, G ^a   Gillet, J ^b   Cloquemin, G ^b   Boudon Padieu, Elisabeth ^a,c  

^a CEMAGREF   (France)

^b Laboratoire National de la Protection des Vegetaux   (France)

^c UNIVERSITÉ DE BOURGOGNE   (France)

Author keywords

16S rXII; 16SrV; Bois noir; Detection; Elm yellows; Flavescence dor e; Multiplex PCR; Non ribosomal DNA; Phytoplasma; Primers; Quarantine; Stolbur; Vergilbungskrankheit

Indexed keywords

FLAVESCENCE; FLAVESCENCE DOREE; FUNGI; HEXAPODA; PHYTOPLASMA; ULMUS; VITIS;

EID: 0142060033     PISSN: 00427500     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (55)

1. ALMA, A.; 2002: Diffusione di Scaphoideus titanus Ball in Italia. ATTI Giornate Fitopatol. 2002, 51-53.
2. ALMA, A.; 2002: The role of Hyalesthes obsoletus Signoret (Homoptera Cixiidae) in the transmission of grapevine Bois noir in Italy. Atti II Incontro Nazionale sulle Malattie da Fitoplasmi, Roma, Istituto Sperimentale per la Patologia Vegetale, 3-4 ottobre 2002, 57-58.
3. ANGELINI, E.; CLAIR, D.; BORGO, M.; BERTACCINI, A.; BOUDON-PADIEU, E.; 2001: Flavescence dorée in France and Italy - Occurrence of closely related phytoplasma isolates and their near relationship to Palatinate grapevine yellows and an alder yellows phytoplasma. Vitis 40, 79-86.
4. ANGELINI, E.; NEGRISOLO, E.; CLAIR, D.; BORGO, M.; BOUDON-PADIEU, E.; 2003: Phylogenetic relationships among Flavescence dorée isolates and related phytoplasmas determined by Heteroduplex Mobility Assay and sequences of ribosomal and non-ribosomal DNA. Plant Pathol. 52 (in press).
5. BAGGIOLINI, M.; CANEVASCINI, V.; CACCIA, R.; TENCALLA, Y.; SOBRIO, G.; 1968: Présence dans le vignoble du tessin d'une cicadelle néarctique nouvelle pour la Suisse, Scaphoideus littoralis Ball. (Homoptera: Jassidae), vecteur possible de la Flavescence dorée. Mitt. Schweiz. Entomol. Ges. 60, 270-275.
6. BATLLE, A.; LAVIÑA, A.; CLAIR, D.; LARRUE, J.; KUSZALA, C.; BOUDON-PADIEU, E.; 1997: Detection of Flavescence dorée in grapevine in Northern Spain. Vitis 36, 211-212.
7. BATLLE, A.; MARTİNEZ, M. A.; LAVIÑA, A.; 2000: Occurrence, distribution and epidemiology of Grapevine yellows in Spain. Eur. J. Plant Pathol. 106, 811-816.
8. BERTACCINI, A.; VIBIO, M.; STEFANI, E.; 1995: Detection and molecular characterization of phytoplasmas infecting grapevine in Liguria (Italy). Phytopathol. mediterr. 34, 137-141.
9. BIANCO, P. A.; DAVIS, R. E.; CASATI, P.; FORTUSINI, A.; 1996: Prevalence of aster yellows (AY) and elm yellows (EY) group phytoplasmas in symptomatic grapevines in three areas of northern Italy. Vitis 35, 195-199.
10. BORGO, M.; ANGELINI, E.; 2002: Diffuzione della Flavescenza dorata della vite in Italia e relazioni con vitigni, pratiche agronomiche et materiali di propagazione. ATTI Giornate Fitopatol. 2002, 35-50.
11. BOUDON-PADIEU, E.; 2000: La cicadelle vectrice de la Flavescence dorée, Scaphoideus titanus Ball, 1932. In: B. WALTER (Ed.): Ravageurs de la Vigne, 110-120. FERET, Bordeaux.
12. BOUDON-PADIEU, E.; 2002: Flavescence dorée of the grapevine: Knowledge and new developments in epidemiology, etiology and diagnosis. ATTI Giornate Fitopatol. 2002, 15-34.
13. BOUDON-PADIEU, E.; BÉJAT, A.; CLAIR, D.; LARRUE, J.; BORGO, M.; BERTOTTO, L.; ANGELINI, A.; 2003: Grapevine yellows: Comparison of different procedures for DNA extraction and amplification with PCR for routine diagnosis of phytoplasma in grapevine. Vitis 42, 141-149.
14. BOUDON-PADIEU, E.; MAIXNER, M.; 1998: Jaunisses de la Vigne: État des connaissances et des méthodes de lutte / Grapevine Yellows: Current knowledge and control methods. Bull. O I V (Off. Int. Vigne Vin) 71 (809-810), 572-607.
15. BOURQUIN, L.; SCHMID, A.; DEMEYER, J.; GAZELLES, O.; RAMEL, M. E.; GUGERLI, P.; 2000: Confirmation of the presence of stolbur type yellows in Swiss vineyards by molecular diagnosis of grapevine. 13th Meeting of ICVG, Adelaide (Australia), March 12-17, 2000. Extended abstracts, 111-112. Univ. Adelaide and CSIRO Publ.
16. CARRARO, L.; LOI, N.; KUSZALA, C.; CLAIR, D.; BOUDON-PADIEU, E.; REFATTI, E.; 1994: On the ability-inability of Scaphoideus titanus Ball to transmit different grapevine yellows agents. Vitis 33, 231-234.
17. CAUDWELL, A.; KUSZALA, C.; 1992: Mise au point d'un test ELISA sur les tissus de vignes atteintes de Flavescence dorée. Res. Microbiol. 143, 791-806.
18. CAUDWELL, A,; KUSZALA, C.; LARRUE, J.; BACHELIER, J. C.; 1972: Transmission de la Flavescence dorée de la fève à la fève par des cicadelles des genres Euscelis et Euscelidius. Intervention possible de ces insectes dans l'épidémiologie du Bois noir en Bourgogne. Proc. Congr. ICVG, Colmar, 1970. Ann. Rev. Phytopathol. N° hors série, 181-189.
19. CAUDWELL, A.; LARRUE, J.; KUSZALA, C.; BACHELIER, J. C.; 1971: Pluralité des Jaunisses de la vigne. Ann. Rev. Phytopathol. 3, 95-105.
20. CAUDWELL, A.; LARRUE, J.; TASSART, V; BOIDRON, R.; GRENAN, S.; LEGUAY, M.; BERNARD, P.; 1994: Caractère porteur de la Flavescence dorée chez les vignes porte-greffes en particulier le 3309 C et le Fercal. Agronomie 14, 83-94.
21. CLAIR, D.; FRELET, A.; AUBERT, G.; COLLIN, E.; BOUDON-PADIEU, E.; 2000: Improved detection of flavescence dorée and related phytoplasma in the elm yellows group in difficult material, with specific PCR primers that amplify a variable non ribosomal DNA fragment. 13th Meeting of ICVG, Adelaide (Australia), March 12-17, 2000. Extended abstracts, 101-102. Univ. Adelaide and CSIRO Publ.
22. CLERC, L.; LINDER, C.; GUNTHART, H.; 1997: Première observation en Suisse romande de la cicadelle Scaphoideus titanus Ball (Homoptera: Jassidae), vecteur de la flavescence dorée de la vigne. Rev. Suisse Vitic. Arboric. Hortic. 29, 245-247.
23. CONSTABLE, F. E.; WHITING, J. R.; GIBB, K. S.; SYMONS, R. H. 2002: A new grapevine yellows phytoplasma from the Buckland Valley of Victoria, Australia. Vitis 41, 147-153.
24. DAIRE, X.; BOUDON-PADIEU, E.; BERVILLÉ, A.; SCHNEIDER, B.; CAUDWELL, A.; 1992: Cloned DNA probes for detection of grapevine Flavescence dorée mycoplasma-like organism (MLO). Ann. Appl. Biol. 121, 95-103.
25. DAIRE, X.; CLAIR, D.; LARRUE, J.; BOUDON-PADIEU, E.; 1997 a: Survey for grapevine yellows in diverse European countries and Israel. Vitis 36, 53-54.
26. DAIRE, X.; CLAIR, D.; LARRUE, J.; BOUDON-PADIEU, E.; ALMA, A.; ARZONE, A.; CARRARO, L.; OSLER, R.; REFATTI, E.; GRANATA, G.; CREDI, R.; TANNE, E.; PEARSON, R.; CAUDWELL, A.; 1993: Occurrence of diverse MLOs in tissues of grapevine affected by grapevine yellows in different countries. Vitis 32, 247-248.
27. DAIRE, X.; CLAIR, D.; REINERT, W.; BOUDON-PADIEU, E.; 1997 b: Detection and differentiation of grapevine yellows phytoplasmas belonging to the elm yellows group and to the stolbur subgroup by PCR amplification of non-ribosomal DNA. Eur. J. Plant Pathol. 103, 507-514.
28. DAVIS, R. E.; DALLY, E. L.; TANNE, E.; RUMBOS, I. C.; 1997: Phytoplasmas associated with grapevine yellows in Israel and Greece belong to the stolbur phytoplasma subgroup, 16S rXII-A. J. Plant Pathol. 79, 181-187.
29. FIRRAO, G.; PALMANO, S.; MALOSSINI, G.: TOMADA, I.; CARPANELLI, A.; DAZZAN, M.; FRAUSIN, C.; 1999: Monitoring grapevine yellows in North-Eastern Italy. First Internet conference on phytopathogenic mollicutes. http://www.uniud.it/phytoplasma/pap/firr4200.html
30. GATINEAU, F.; LARRUE, J.; CLAIR, D.; LORTON, F.; RICHARD-MOLARD, M.; BOUDON-PADIEU, E.; 2001: A new natural planthopper vector of stolbur phytoplasma in the genus Pentastiridius (Hemiptera: Cixiidae). Eur. J. Plant Pathol. 107, 263-271.
31. GIBB, K. S.; CONSTABLE, F. E.; MORAN, J. R.; PADOVAN, A. C.; 1999: Phytoplasmas in Australian grapevines - detection, differentiation and associated diseases. Vitis 38, 107-114.
32. LAVIÑA, A.; BATLLE, A.; LARRUE, J.; DAIRE, X.; CLAIR, D.; BOUDON-PADIEU, E.; 1995: First report of grapevine bois noir phytoplasma in Spain. Plant Dis. 79, 1075.
33. LEE, I. M.; GUNDERSEN-RINDAL, D. E.; DAVIS, R. E.; BARTOSZYK, I. M.; 1998: Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int. J. Syst. Bacteriol. 48, 1153-1169.
34. LEE, I. M.; GUNDERSEN, D. E.; HAMMOND, R. W.; DAVIS, R. E.; 1994: Use of Mycoplasma-like organism (MLO) group-specific oligonucleoide primers for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84, 559-566.
35. MAIXNER, M.; 1994: Transmission of German grapevine yellows (Vergilbungskrankheit) by the planthopper Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae). Vitis 33, 103-104.
36. MAIXNER. M.; DAIRE, X.; BOUDON-PADIEU, E.; LAVIÑA, A.; BATLLE, A.; REINERT, W.; 1997: Phytoplasmas. In: Les colloques, INRA Editions N° 86. Sanitary Selection of the grapevine. Protocols for detection of viruses and virus-like diseases, 183-195.
37. MAIXNER, M.; DARIMONT, H.; REINERT, W.; 2000 a: Course of infestation by grapevine yellows in vineyards after replanting. 13th Meeting of ICVG, Adelaide (Australia), March 12-17, 2000. Extended abstracts, 109-110. Univ. Adelaide and CSIRO Publ.
38. MAIXNER, M.; REINERT, W.; DARIMONT, H.; 2000 b: Transmission of grapevine yellows by Oncopsis alni (Schrank) (Auchenorrhyncha: Macropsinae). Vitis 39, 83-84.
39. MARCONE, C.; RAGOZZINO, A.; CREDI, R.; SEEMÜLLER, E.; 1996: Detection and characterization of phytoplasmas infecting grapevine in southern Italy and their genetic relatedness to other grapevine yellows phytoplasmas. Phytopathol. mediterr. 35, 207-213.
40. MARTINI, M.; BOTTI, S.; MARCONE, C.; MARZACHÌ, C.; CASATI, P.; BIANCO, P. A.; BENEDETTI, R.; BERTACCINI, A.; 2002: Genetic variability among Flavescence dorée phytoplasmas from different origins in Italy and France. Mol. Cell. Probes 16, 197-203.
41. MORI, N.; BRESSAN, A.; MARTINI, M.; GUADAGNINI, M.; GIROLAMI, V.; BERTACCINI, A.; 2002: Experimental transmission by Scaphoideus titanus Ball of two Flavescence dorée-type phytoplasmas. Vitis 41, 99-102.
42. PADOVAN, A.; GIBB, K.; DAIRE, X.; BOUDON-PADIEU, E.; 1996: A comparison of the phytoplasma associated with Australian grapevine yellows to other phytoplasmas in grapevine. Vitis 35, 189-194.
43. PEARSON, R. C.; POOL, R. M.; GONSALVES, D.; GOFFINET, M. C.; 1985: Occurrence of flavescence dorée like symptoms on «White Riesling» grapevines in New York, USA. Phytopathol. mediterr. 24, 82-87.
44. PRINCE, J. P.; DAVIS, R. E.; WOLF, T. K.; LEE, I. M.; MOGEN, B. D.; DALLY, E. L.; BERTACCINI, A.; CREDI, R.; BARBA, M.; 1993: Molecular detection of diverse mycoplasmalike organisms (MLOs) associated with grapevine yellows and their classification with aster yellows, X-disease, and elm yellows MLOs. Phytopathology 83, 1130-1137.
45. QUARTAU, J. A.; GUIMARÃES, J. M.; ANDRÉ, G.; 2001: On the occurrence in Portugal of the nearctic Scaphoideus titanus Ball (Homoptera, Cicadellidae), the natural vector of the grapevine «Flavescence dorée» (FD). IOBC/wprs Bull. 24, 273-276.
46. REINERT, W.; 1999: Detektion und Differenzierung rebpathogener Phytoplasmen (Mollicutes, Eubacteria) in Deutschland unter Berü cksichtigung phytopathologischer Aspekte. Diss. Technische Univ. Darmstadt.
47. SCHVESTER, D.; CARLE, P.; MOUTOUS, G.; 1963: Transmission de la flavescence dorée de la vigne par Scaphoideus littoralis Ball. Ann. Epiphyties 14, 175-198.
48. SEEMÜLLER, E.; MARCONE, C.; LAUER, U.; RAGOZZINO, A.; GÖSCHL, M.; 1998: Current status of molecular classification of the phytoplasmas. J. Plant Pathol 80, 3-26.
49. SELJAK, G.; 2002: Non-European Auchenorryncha (Hemiptera) and their geographic distribution in Slovenia. Acta Entomol. Slovenica 10, 97-101.
50. SFORZA, R.; CLAIR, D.; DAIRE, X.; LARRUE, J.; BOUDON-PADIEU, E.; 1998: The role of Hyalesthes obsoletus (Hemiptera: Cixiidae) in the occurrence of Bois noir of grapevine in France. J. Phytopathol. 146, 549-556.
51. SKORIC, D; SARIC, A.; VIBIO, M.; MURARI, E.; KRAJACIC, M.; BERTACCINI, A.; 1998: Molecular identification and seasonal monitoring of phytoplasmas infecting Croatian grapevines. Vitis 37, 171-175.
52. TANNE, E.; ORENSTEIN, S.; 1997: Identification and typing of grapevine phytoplasma amplified by graft transmission to periwinkle. Vitis 36, 35-38.
53. VARGA, K.; KOLBER, M.; MARTINI, M; PONDRELLI, M; EMBER, I.; TÕKÉS, G.; LÁZÁR, J.; MIKULÁS, J.; PAPP, E., SZENDREY, G.; SCHWEIGERT, Á.; BERTACCINI, A.; 2000: Phytoplasma identification in Hungarian grapevines by two nested-PCR systems. 13th Meeting of ICVG, Adelaide (Australia), March 12-17, 2000. Extended abstracts, 113-115. Univ. Adelaide and CSIRO Publ.
54. WEBER, A.; MAIXNER, M.; 1998: Habitat requirements of Hyalesthes obsoletus (Auchenorrhyncha: Cixiidae) and approaches to control this planthopper in vineyards. IOBC wprs Bull. 21, 77-78.
55. WOLF, T. K.; PRINCE, J. P.; DAVIS, R. E.; 1994: Occurrence of a grapevine yellows in Virginia vineyards. Plant Dis. 78, 208.