A modified Sindbis vector for prolonged gene expression in neurons

Journal of Neurophysiology

Volumn 90, Issue 4, 2003, Pages 2741-2745

  Jeromin, Andreas ^a   Yuan, Li Lian ^a   Frick, Andreas ^a   Pfaffinger, Paul ^a   Johnston, Daniel ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; ION CHANNEL; VIRUS VECTOR;

ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CYTOTOXICITY; ELECTROPHYSIOLOGY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE MUTATION; HIPPOCAMPUS; IMAGING; LIFE CYCLE; MAMMAL; NERVE CELL; NEUROBIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RAT; SINDBIS VIRUS; SPINE;

ANIMALS; ANIMALS, NEWBORN; GENE EXPRESSION REGULATION, VIRAL; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; NEURONS; ORGAN CULTURE TECHNIQUES; RATS; RATS, SPRAGUE-DAWLEY; SINDBIS VIRUS;

EID: 0142058587     PISSN: 00223077     EISSN: None     Source Type: Journal    
DOI: 10.1152/jn.00464.2003     Document Type: Article

References (11)

1. Dryga S, Dryga OA, and Schlesinger S. Identification of mutations in a Sindbis virus variant able to establish persistent infection in BHK cells: the importance of a mutation in the nsP2 gene. Virology 22: 74-83, 1997.
2. Ehrengruber MU. Alphaviral vectors for gene transfer into neurons. Mol Neurobiol 26: 183-201, 2002.
3. 2+ by small oblique dendrites of CA1 pyramidal neurons. J Neurosci 23: 3243-3250, 2003.
4. Frolov I, Frolov I, Agapov E, Hoffman TA Jr, Pragai BM, Lippa M, Schlesinger S, and Rice CM. Selection of RNA replicons capable of persistent non-cytopathic replication in mammalian cells. Proc Natl Acad Sci USA 73: 3854-3865, 1999.
5. Huang HV and Schlesinger S. Gene Transfer and Expression in Mammalian Cells. Boca Raton, FL: CRC, 2003.
6. Johnston D, Hoffman DA, Magee JC, Poolos NP, Watanabe S, Colbert CM, and Migliore M. Dendritic potassium channels in hippocampal pyramidal neurons. J Physiol 525: 75-81, 2000.
7. Kim J, Dittgen T, Kolleker A, Waters DJ, Schlesinger S, Seeburg PH, and Osten P. Evaluation of the Sindbis virus (nsp2-P726S) vector as a tool for heterologous expression in neurons. Soc Neurosci Abst 902.7, 2002.
8. Shi S, Hayashi Y, Esteban JA, and Malinow R. Subunit-specifc rules governing AMPA receptor trafficking to synapes in hippocampal pyramidal neurons. Cell 105: 331-343, 2001.
9. Stoppini L, Buchs PA, and Muller D. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 37: 173-182, 1991.
10. Washbourne P and McAllister AK. Techniques for gene transfer into neurons. Curr Opin Neurobiol 12: 566-573, 2002.
11. + channels in hippocampus involves a mitogen-activated protein kinase pathway. J Neurosci 22: 4860-4868, 2002.