Conditional Survival as a Selection Strategy to Identify Plant-Inducible Genes of Pseudomonas syringae

Applied and Environmental Microbiology

Volumn 69, Issue 10, 2003, Pages 5793-5801

  Marco, Maria L ^a,b,c   Legac, Jennifer ^a,d   Lindow, Steven E ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b TOP INSTITUTE FOOD AND NUTRITION   (Netherlands)

^c NIZO FOOD RESEARCH   (Netherlands)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CLONING; DNA; FLUORESCENCE; GENES; PLANTS (BOTANY);

PROMOTER ACTIVITY;

MICROBIOLOGY;

METHIONINE;

ARTICLE; BACTERIAL GROWTH; CELL SURVIVAL; DNA LIBRARY; GENE INDUCTION; GENE TARGETING; GENETIC IDENTIFICATION; GENETIC SCREENING; GENETIC SELECTION; MOLECULAR CLONING; NONHUMAN; PLANT LEAF; PROMOTER REGION; PSEUDOMONAS SYRINGAE; REPORTER GENE; SEQUENCE ANALYSIS;

ACETYLTRANSFERASES; BACTERIAL PROTEINS; CULTURE MEDIA; GENE EXPRESSION REGULATION, BACTERIAL; METHIONINE; PHASEOLUS; PLANT DISEASES; PLANT LEAVES; PROMOTER REGIONS (GENETICS); PSEUDOMONAS SYRINGAE; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PHASEOLUS (ANGIOSPERM); PSEUDOMONAS; PSEUDOMONAS SYRINGAE;

EID: 0142040925     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.10.5793-5801.2003     Document Type: Article

References (43)

1. Andersen, G. L., G. A. Beattie, and S. E. Lindow. 1998. Molecular characterization and sequence of a methionine biosynthetic locus from Pseudomonas syringae. J. Bacteriol. 180:4497-4507.
2. Angelichio, M. J., and A. Camilli. 2002. In vivo expression technology. Infect. Immun. 70:6518-6523.
3. Barre, F. X., B. Soballe, B. Michel, M. Aroyo, M. Robertson, and D. Sherratt. 2001. Circles: the replication-recombination-chromosome segregation connection. Proc. Natl. Acad. Sci. USA 98:8189-8195.
4. Beattie, G. A., and S. E. Lindow. 1999. Bacterial colonization of leaves: a spectrum of strategies. Phytopathology 89:353-359.
5. Beattie, G. A., and S. E. Lindow. 1994. Comparison of the behavior of epiphytic fitness mutants of Pseudomonas syringae under controlled and field conditions. Appl. Environ. Microbiol. 60:3799-3808.
6. Beattie, G. A., and S. E. Lindow. 1994. Survival, growth, and localization of epiphytic fitness mutants of Pseudomonas syringae on leaves. Appl. Environ. Microbiol. 60:3790-3798.
7. Boch, J., V. Joardar, L. Gao, T. L. Robertson, M. Lim, and B. N. Kunkel. 2002. Identification of Pseudomonas syringae pv. tomato genes induced during infection of Arabidopsis thaliana. Mol. Microbiol. 44:73-88.
8. Camilli, A., D. T. Beattie, and J. J. Mekalanos. 1994. Use of genetic recombination as a reporter of gene expression. Proc. Natl. Acad. Sci. USA 91: 2634-2638.
9. Camilli, A., and J. J. Mekalanos. 1995. Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection. Mol. Microbiol. 18:671-683.
10. Chiang, S. L., J. J. Mekalanos, and D. W. Holden. 1999. In vivo genetic analysis of bacterial virulence. Annu. Rev. Microbiol. 53:129-154.
11. Cirvilleri, G., and S. E. Lindow. 1994. Differential expression of genes of Pseudomonas syringae on leaves and in culture evaluated with random genomic lux fusions. Mol. Ecol. 3:249-257.
12. Ditta, G., S. Stanfield, D. Corbin, and D. R. Helinski. 1980. Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77:7347-7351.
13. Hamer, L., T. M. DeZwaan, M. V. Montenegro-Chamorro, S. A. Frank, and J. E. Hamer. 2001. Recent advances in large-scale transposon mutagenesis. Curr. Opin. Chem. Biol. 5:67-73.
14. Heithoff, D. M., C. P. Conner, P. C. Hanna, S. M. Julio, U. Hentschel, and M. J. Mahan. 1997. Bacterial infection as assessed by in vivo gene expression. Proc. Natl. Acad. Sci. USA 94:934-939.
15. Hendrickson, E. L., P. Guevera, A. Penaloza-Vazquez, J. Shao, C. Bender, and F. M. Ausubel. 2000. Virulence of the phytopathogen Pseudomonas syringae pv. Maculicola is rpoN dependent. J. Bacteriol. 182:3498-3507.
16. Hirano, S. S., E. V. Nordheim, D. C. Arny, and C. D. Upper. 1982. Lognormal distribution of epiphytic bacterial populations on leaf surfaces. Appl. Environ. Microbiol. 44:695-700.
17. Huber, K. E., and M. K. Waldor. 2002. Filamentuous phage integration requires the host recombinases XerC and XerD. Nature 417:656-659.
18. King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301-307.
19. Lee, S. H., and A. Camilli. 2000. Novel approaches to monitor bacterial gene expression in infected tissue and host. Curr. Opin. Microbiol. 3:97-101.
20. Lindgren, P. B., R. Frederick, A. G. Govindarajan, N. J. Panopoulos, B. J. Staskawicz, and S. E. Lindow. 1989. An ice nucleation reporter gene system: identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO J. 8:1291-1301.
21. Lindow, S. E. 1993. Novel method for identifying bacterial mutants with reduced epiphytic fitness. Appl. Environ. Microbiol. 59:1586-1592.
22. Lindow, S. E., G. Andersen, and G. A. Beattie. 1993. Characteristics of insertional mutants of Psuedomonas syringae with reduced epiphytic fitness. Appl. Environ. Microbiol. 59:1593-1601.
23. Loper, J. E., and S. E. Lindow. 1987. Lack of evidence for in situ fluorescent pigment production by Pseudomonas syringae pv. syringae on bean leaf surfaces. Phytopathology 77:1449-1454.
24. Mahan, M. J., D. M. Heithoff, R. L. Sinsheimer, and D. A. Low. 2000. Assessment of bacterial pathogenesis by analysis of gene expression in the host. Annu. Rev. Genet. 34:139-164.
25. Mahan, M. J., J. M. Slauch, and J. J. Mekalanos. 1993. Selection of bacterial virulence genes that are specifically induced in host tissues. Science 259:686-688.
26. Mahan, M. J., J. W. Tobias, J. M. Slauch, P. C. Hanna, R. J. Collier, and J. J. Mekalanos. 1995. Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc. Natl. Acad. Sci. USA 92:669-673.
27. Mecsas, J. 2002. Use of signature-tagged mutagenesis in pathogenesis studies. Curr. Opin. Microbiol. 5:33-37.
28. Miller, W. G., J. H. J. Leveau, and S. E. Lindow. 2000. Improved gfp and inaZ broad-host-range promoter-probe vectors. Mol. Plant-Microbe Interact. 13: 1243-1250.
29. Morgan, J. V., and H. B. Tukey. 1964. Characterization of leachate from plant foliage. Plant Physiol. 39:590-593.
30. Osbourn, A. E., C. E. Barber, and M. J. Daniels. 1987. Identification of plant-induced genes of the bacterial pathogen Xanthomonas campestris pathovar campestris using a promoter-probe plasmid. EMBO J. 6:23-28.
31. Rainey, P. B. 1999. Adaptation of Pseudomonas fluorescens to the plant rhizosphere. Environ. Microbiol. 1:243-257.
32. Rainey, P. B., and G. M. Preston. 2000. In vivo expression technology strategies: valuable tools for biotechnology. Curr. Opin. Biotechnol. 11:440-444.
33. Richmond, C. S., J. D. Glasner, R. Mau, H. Jin, and F. R. Blattner. 1999. Genome-wide expression profiling in Escherichia coli K-12. Nucleic Acids Res. 27:3821-3835.
34. Sambrook, J., T. Maniatis, and E. F. Fritsch. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Sekowska, A., H. F. Kung, and A. Danchin. 2000. Sulfur metabolism in Escherichia coli and related bacteria: facts and fiction. J. Mol. Microbiol. Biotechnol. 2:145-177.
36. Tao, H., C. Bausch, C. Richmond, F. R. Blattner, and T. Conway. 1999. Functional genomics: expression analysis of Escherichia coli growing on minimal and rich media. J. Bacteriol. 181:6425-6440.
37. Valdivia, R. H., and S. Falkow. 1996. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22:367-378.
38. Valdivia, R. H., and S. Falkow. 1997. Fluorescence-based isolation of bacterial genes expressed within host cells. Science 277:2007-2011.
39. Wang, J. Y., A. Mushegian, S. Lory, and S. G. Jin. 1996. Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection. Proc. Natl. Acad. Sci. USA 93:10434-10439.
40. Watson, R. J. 1990. Analysis of the C4-dicarboxylate transport genes of Rhizobium meliloti: nucleotide sequence and deduced products of dctA, dctB, and dctD. Mol. Plant-Microbe Interact. 3:174-181.
41. Wei, Y., J.-M. Lee, C. Richmond, F. R. Blattner, J. A. Rafalski, and R. A. LaRossa. 2001. High-density microarray-mediated gene expression profiling of Escherichia coli. J. Bacteriol. 183:545-556.
42. Weibull, J., F. Ronquist, and S. Brishammar. 1990. Free amino acid composition of leaf exudates and phloem sap: a comparative study in oats and barley. Plant Physiol. 92:222-226.
43. Wilson, M., S. S. Hirano, and S. E. Lindow. 1999. Location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leal. Appl. Environ. Microbiol. 65:1435-1443.37.