Purification of recombinant human cPLA2γ and identification of C-terminal famesylation, proteolytic processing, and carboxymethylation by MALDI-TOF-TOF analysis

Biochemistry

Volumn 42, Issue 40, 2003, Pages 11798-11807

  Jenkins, Christopher M ^a   Han, Xianlin ^a   Yang, Jingyue ^a   Mancuso, David J ^a   Sims, Harold F ^a   Muslin, Anthony J ^a   Gross, Richard W ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; CHEMICAL MODIFICATION; ENZYME KINETICS; MASS SPECTROMETRY; SUBSTRATES;

COVALENT STRUCTURE;

GENETIC ENGINEERING;

CALCIUM; CYSTEINE; CYTOSOLIC PHOSPHOLIPASE A2 GAMMA; HISTIDINE; LYSOPHOSPHOLIPASE; MEVALONOLACTONE; NICKEL; PALMITOYL COENZYME A; PHOSPHOLIPASE A2; PRENYLAMINE; STATIN; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE; CHEMICAL REACTION; COLUMN CHROMATOGRAPHY; CYTOSOL; ENZYME ACTIVITY; ENZYME PURIFICATION; HYDROLYSIS; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; INSECT; ISOTOPE LABELING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METHYLATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING; SILVER STAINING; SOLUBILIZATION;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CYTOSOL; ENDOPEPTIDASES; ENZYME ACTIVATION; ENZYME INHIBITORS; HUMANS; HYDROLYSIS; LYSOPHOSPHOLIPASE; METHYLATION; MOLECULAR SEQUENCE DATA; PALMITOYL COENZYME A; PEPTIDE FRAGMENTS; PHOSPHOLIPASES A; PROTEIN ISOPRENYLATION; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPODOPTERA;

INSECTA;

EID: 0141988875     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi034611q     Document Type: Article

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