Major pathological mechanisms of acute pancreatitis are prevented by N-acetylcysteine

Digestion

Volumn 68, Issue 1, 2003, Pages 34-40

  Sevillano, S ^a   De La Mano, A M ^a   De Dios, Isabel ^a   Ramudo, L ^a   Manso, M A ^a  

^a UNIVERSITY OF SALAMANCA   (Spain)

Author keywords

Acinar cells; Acute pancreatitis; Calcium; Digestive enzymes; N acetylcysteine; Oxidative stress

Indexed keywords

ACETYLCYSTEINE; AMYLASE; GLUTATHIONE; MALONALDEHYDE; TRYPSINOGEN;

ACINAR CELL; ACUTE PANCREATITIS; AMINO ACID BLOOD LEVEL; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CALCIUM CELL LEVEL; CALCIUM HOMEOSTASIS; CELL LEVEL; CONTROLLED STUDY; CYTOSOL; DRUG EFFECT; ENZYME ACTIVITY; MALE; NONHUMAN; OXIDATIVE STRESS; PATHOGENESIS; PRIORITY JOURNAL; PROPHYLAXIS; RAT; TREATMENT OUTCOME;

ACETYLCYSTEINE; ACUTE DISEASE; ANIMALS; FREE RADICAL SCAVENGERS; MALE; MICROSCOPY, ELECTRON; OXIDATIVE STRESS; PANCREAS; PANCREATITIS; RATS; RATS, WISTAR;

EID: 0141924119     PISSN: 00122823     EISSN: None     Source Type: Journal    
DOI: 10.1159/000073223     Document Type: Article

References (39)

1. Bialek R, Willemer S, Arnold R, Adler G: Evidence of intracellular activation of serine proteases in acute cerulein-induced pancreatitis in rats. Scand J Gastroenterol 1991;26:190-196.
2. Leach SD, Modlin IM, Scheele GA, Gorelick FS: Intracellular activation of digestive zymogens in rat pancreatic acini: Stimulation by high doses of cholecystokinin. J Clin Invest 1991;87:362-366.
3. Saluja AK, Donovan EA, Yamanaka K, Yamaguchi Y, Hofbauer B, Steer ML: Cerulein-induced in vitro activation of trypsinogen in rat pancreatic acini is mediated by cathepsin B. Gastroenterology 1997;113:304-310.
4. Raraty M, Ward J, Erdemli G, Vaillant C, Neoptolemos JP, Sutton R, Petersen OH: Calcium-dependent enzyme activation and vacuole formation in the apical granular region of pancreatic acinar cells. Proc Natl Acad Sci USA 2000;97:13126-13131.
5. Saluja AK, Bhagat L, Lee HS, Bhatia M, Frossard JL, Steer ML: Secretagogue-induced digestive enzyme activation and cell injury in rat pancreatic acini. Am J Physiol 1999;276:G835-G842.
6. Krüger B, Albrecht E, Lerch MM: The role of intracellular calcium signalling in premature protease activation and the onset of pancreatitis. Am J Pathol 2000;157:43-50.
7. Grady T, Saluja AK, Kaiser A, Steer ML: Edema and intrapancreatic trypsinogen activation precede glutathione depletion during caerulein pancreatitis. Am J Physiol 1996;271:G20-G26.
8. Rao KN, Tuma J, Lombardi B: Acute hemorrhagic pancreatic necrosis in mice: Intraparenchymal activation of zymogens and other enzyme changes in pancreas and serum. Gastroenterology 1976;70:720-726.
9. Rinderknecht H: Activation of pancreatic zymogens: Normal activation, premature intrapancreatic activation, protective mechanisms against inappropriate activation. Dig Dis Sci 1986;31:314-321.
10. Yamaguchi H, Kimura T, Nimura K, Nawata H: Activation of proteases in cerulein-induced pancreatitis. Pancreas 1989;4:565-571.
11. Uruñuela A, Manso MA, de la Mano AM, Sevillano S, Orfao A, de Dios I: Asynchronous impairment of calcium homoeostasis in different acinar cells after pancreatic duct obstruction in rat. Clin Sci (Lond) 2002;102:615-622.
12. De Dios I, Uruñuela A, Orfao A, Manso MA: Cholecystokinin antagonist L364,718 induces alterations in acinar cells that prevent improvement of acute pancreatitis induced by obstruction. Dig Dis Sci 2002;47:1800-1809.
13. De la Mano AM, Sevillano S, de Dios I, Vicente S, Manso MA: Low enzyme content in the pancreas does not reduce the severity of acute pancreatitis induced by bile-pancreatic duct obstruction. Mol Cell Biochem 2002;240:75-81.
14. Ward JB, Sutton R, Jenkins SA, Petersen OH: Progressive disruption of acinar cell calcium signaling as an early feature of cerulein-induced pancreatitis in mice. Gastroenterology 1996;111:481-491.
15. Sanfey H, Bulkley GB, Cameron JL: The role of oxygen-derived free radicals in the pathogenesis of acute pancreatitis. Ann Surg 1984;200:405-413.
16. Nonaka A, Manabe T, Kyogoku T, Tamamura K, Tobe T: Changes in lipid peroxide and oxygen radical scavengers in cerulein-induced acute pancreatitis. Digestion 1990;47:130-137.
17. Reinheckel T, Nedelev B, Prause J, Augustin W, Schulz HU, Lippert H, Halangk W: Occurrence of oxidatively modified proteins: An early event in experimental acute pancreatitis. Free Radic Biol Med 1998;24:393-400.
18. Wiseman H, Halliwell B: Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer. Biochem J 1996;313:17-29.
19. Rau B, Poch B, Gansauge F, Bauer A, Nüssler AK, Nevalainen T, Schoenberg MH, Beger HG: Pathophysiologic role of oxygen free radicals in acute pancreatitis: Initiating event or mediator of tissue damage? Ann Surg 2000;23: 352-360.
20. Demols A, van Laethem JL, Quertinmont E, Legros F, Louis H, Le Moine O, Devière I: N-acetylcysteine decreases severity of acute pancreatitis in mice. Pancreas 2000;20:161-169.
21. Niedereau C, Niedereau M, Borchard F, Ude K, Lüthen R, Strohmeyer G, Ferrell LD, Grendell JH: Effects of antioxidants and free radical scavengers in three different models of acute pancreatitis. Pancreas 1992;7:486-492.
22. Kruse P, Anderson ME, Loft S: Minor role of oxidative stress during intermediate phase of acute pancreatitis in rats. Free Radic Biol Med 2001;30:309-317.
23. Cotgreave IA: N-acetylcysteine: Pharmacological considerations and experimental and clinical applications. Adv Pharmacol 1997;38:205-227.
24. Uruñuela A, Manso MA, Pinto RM, Orfao A, de Dios I: Enzyme load in pancreatic acinar cells is increased in the early stages of acute pancreatitis induced by duct obstruction in rats. Clin Sci (Lond) 2000;98:143-150.
25. De Dios I, Rodriguez A, Garcia-Montero A, Orfao A, Manso MA: Enzyme changes in zymogen granules and in pancreatic secretion throughout longterm CCK treatment. Peptides 1997;18:101-111.
26. Hickson JC: The secretion of pancreatic juice in response to stimulation of the vagus nerves in the pig. J Physiol (Lond) 1970;206:275-297.
27. Tietze F: Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 1969;27:502-522.
28. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-358.
29. Vandenberghe PA, Ceuppens JL: Flowcytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator. J Immunol Methods 1990;127:197-205.
30. Schoenberg MH, Büchler M, Beger HG: The role of oxygen radicals in experimental acute pancreatitis. Free Radic Biol Med 1992;12:515-522.
31. Schulz HU, Niedereau C, Klonowski-Stumpe H, Halangk W, Lüthen R, Lippert H: Oxidative stress in acute pancreatitis. Hepatogastroenterology 1999;46:2736-2750.
32. Uruñuela A, Sevillano S, de la Mano AM, Manso MA, Orfao A, De Dios I: Time-course of oxygen free radical production in acinar cells during acute pancreatitis induced by pancreatic duct obstruction. Biochim Biophys Acta 2002;1588:159-164.
33. Lüthen R, Grendell JH, Niedereau C, Haüssinger D: Trypsinogen activation and glutathione content are linked to pancreatic injury in models of biliary acute pancreatitis. Int J Pancreatol 1998;24:193-202.
34. Czakó L, Takákcs T, Varga IS, Hai DQ, Tiszlavicz L, Hegyi P, Mándi Y, Matkovics B, Lonovics J: The pathogenesis of L-arginine-induced acute necrotizing pancreatitis: inflammatory mediators and endogenous cholecystokinin. J Physiol Paris 2000;94:43-50.
35. Scheele G, Adler G, Kern H: Exocytosis occurs at the lateral plasma membrane of the pancreatic acinar cells during supramaximal secretagogue stimulation. Gastroenterology 1987;92:345-353.
36. Gonzalez A, Schmid A, Salido GM, Camello PJ, Pariente JA: XOD-catalyzed ROS generation mobilizes calcium from intracellular stores in mouse pancreatic acinar cells. Cell Signal 2002;14:153-159.
37. ++ amplifies oxygen radical-induced alterations of the ultrastructure and the energy metabolism of isolated rat pancreatic acinar cells. Digestion 1998;59:175-185.
38. Malorni W, Iosi F, Mirabelli F, Bellomo G: Cytoskeleton as a target in menadione-induced oxidative stress in cultured mammalian cells: Alterations underlying surface bleb formation. Chem Biol Interact 1991;80:217-236.
39. Bragado MJ, San Román JI, Gonzalez A, Garcia LJ, López MA, Calvo JJ: Impairment of intracellular calcium homeostasis in the exocrine pancreas after caerulein-induced acute pancreatitis in the rat. Clin Sci (Lond) 1996;91:365-369.