Interactions of potent multisubstrate analogue inhibitors with purine nucleoside phosphorylase from calf spleen - Kinetic and spectrofluorimetric studies

Nucleosides, Nucleotides and Nucleic Acids

Volumn 22, Issue 5-8, 2003, Pages 1567-1570

  Iwanow, Monika ^a   Magnowska, Lucyna ^a   Yokomatsu, Tsutomu ^b   Shibuya, Shiroshi ^b   Bzowska, Agnieszka ^a  

^a UNIVERSITY OF WARSAW   (Poland)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

Dissociation constants; Fluorescence; Inhibition constants; Multisubstrate analogue inhibitors; Purine nucleoside phosphorylase; Stoichiometry

Indexed keywords

PURINE NUCLEOSIDE PHOSPHORYLASE; PURINE NUCLEOSIDE PHOSPHORYLASE INHIBITOR;

ANIMAL TISSUE; CATTLE; CONFERENCE PAPER; DISSOCIATION CONSTANT; ENZYME INHIBITOR INTERACTION; FLUORESCENCE; KINETICS; NONHUMAN; SPECTROFLUOROMETRY; SPLEEN; STOICHIOMETRY;

ANIMALS; CATTLE; ENZYME INHIBITORS; KINETICS; PURINE-NUCLEOSIDE PHOSPHORYLASE; SPECTROMETRY, FLUORESCENCE; SPLEEN; SUBSTRATE SPECIFICITY;

ANIMALIA; BOS TAURUS;

EID: 0141819152     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-120023035     Document Type: Conference Paper

References (7)

1. Bzowska, A.; Kulikowska, E.; Shugar, D. Purine nucleoside phosphorylases: properties, functions, and clinical aspects. Pharmacol. & Therapeut. 2000, 88, 349-425.
2. Wang, F.; Miles, R.W.; Kicska, G.; Nieves, E.; Schramm, V.L.; Angeletti, R.H. Immucillin-H binding to purine nucleoside phosphorylase reduces dynamic solvent exchange. Protein Sci. 2000, 9, 1660-1668.
3. Bzowska, A. Calf spleen purine nucleoside phosphorylase: complex kinetic mechanism, hydrolysis of 7-methylguanosine, and oligomeric state in solution. Biochim. Biophys. Acta 2002, 1596, 293-317.
4. Halazy, S.; Ehrhard, A.; Danzin, C. 9-(Difluorophosphonoalkyl)guanines as a new class of multisubstrate analogue inhibitors of purine nucleoside phosphorylase. J. Am. Chem. Soc. 1991, 113, 315-317.
5. Yokomatsu, T.; Hayakawa, Y.; Kihara, T.; Koyanagi, S.; Soeda, S.; Shimeno, H.; Shibuya, S. Synthesis and evaluation of multisubstrate analogue inhibitors of purine nucleoside phosphorylases. Bioorg. Med. Chem. 2000, 8, 2571-2579.
6. Eftink, M.R. Fluorescence methods for studying equilibrium macromolecule-ligand interactions. Methods Enzymol. 1997, 278, 221-257.
7. Miles, R.W.; Tyler, P.C.; Furneaux, R.H.; Bagdassarian, C.K.; Schramm, V.L. One-third-the-sites transition-state inhibitors for purine nucleoside phosphorylase. Biochemisty 1998, 37, 8615-8621.