Characterization of the catalytic subunit of the human herpesvirus 6 (HHV-6) DNA polymerase expressed in an in vitro transcription/translation assay

Nucleosides, Nucleotides and Nucleic Acids

Volumn 22, Issue 5-8, 2003, Pages 999-1001

  De Bolle, L ^a   Balzarini, J ^a   De Clercq, E ^a   Naesens, L ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; FOSCARNET; GANCICLOVIR; HUMAN HERPESVIRUS 6 DNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS DNA;

CATALYSIS; CONFERENCE PAPER; ENZYME ANALYSIS; HUMAN HERPESVIRUS 6; IC 50; IN VITRO STUDY; NONHUMAN; REPRODUCIBILITY; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION; TRANSLATION REGULATION; VIRUS REPLICATION; VIRUS RESISTANCE;

ANIMALS; ANTIVIRAL AGENTS; BINDING SITES; CATALYSIS; CLONING, MOLECULAR; CYTOSINE; DIMERIZATION; DNA-DIRECTED DNA POLYMERASE; FOSCARNET; GANCICLOVIR; HERPESVIRUS 6, HUMAN; HUMANS; ORGANOPHOSPHORUS COMPOUNDS; PHOSPHONIC ACIDS; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN SUBUNITS; RABBITS; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

DNA VIRUSES; HERPESVIRIDAE; HUMAN HERPESVIRUS 6;

EID: 0141708682     PISSN: 15257770     EISSN: None     Source Type: Journal    
DOI: 10.1081/NCN-120022722     Document Type: Conference Paper

References (4)

1. Clark, D.A. Human herpesvirus 6. Rev. Med. Virol. 2000, 10, 155-173.
2. Bapat, A.R.; Bodner, A.J.; Ting, R.C.; Cheng, Y.C. Identification and some properties of a unique DNA polymerase from cells infected with human B-lymphotropic virus. J. Virol. 1989, 63, 1400-1403.
3. Cihlar, T.; Fuller, M.D.; Cherrington, J.M. Expression of the catalytic subunit (UL54) and the accessory protein (UL44) of human cytomegalovirus DNA polymerase in a coupled in vitro transcription/translation system. Protein Expr. Purif. 1997, 11, 209-218.
4. Manichanh, C.; Olivier-Aubron, C.; Lagarde, J.P.; Aubin, J.T.; Bossi, P. ; Gautheret-Dejean, A.; Huraux, J.M.; Agut, H. Selection of the same mutation in the U69 protein kinase gene of human herpesvirus-6 after prolonged exposure to ganciclovir in vitro and in vivo. J. Gen. Virol. 2001, 82, 2767-2776.