Expression of the catalytic subunit (UL54)and the accessory protein (UL44) of human cytomegalovirus DNA polymerase in a coupled in vitro transcription/translation system

Protein Expression and Purification

Volumn 11, Issue 2, 1997, Pages 209-218

  Cihlar, Tomas ^a   Fuller, Michael D ^a   Cherrington, Julie M ^a  

^a GILEAD SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALFALFA MOSAIC VIRUS; CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5; MEDICAGO SATIVA;

EID: 0031281931     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0781     Document Type: Article

References (30)

1. Britt W., Alford C. Cytomegalovirus. Fields Virology. 1996;Lippincott-Raven Publishers, Philadelphia. p. 2493-2523.
2. Ho M. Cytomegalovirus: Biology and Infection. 1991;Plenum, New York.
3. Neyts J., De Clercq E. Strategies for the treatment and prevention of cytomegalovirus infections. Int. J. Antimicrob. Agents. 3:1993;187-204.
4. Hitchcock M. J. M., Jaffe H. S., Martin J. C., Stagg R. J. Cidofovir, a new agent with potent anti-herpesvirus activity. Antiviral Chemistry Chemother. 7:1996;115-127.
5. Mar E.-C., Chiou J.-F., Cheng Y.-C., Huang E.-S. Inhibition of cellular DNA polymerase alpha and human cytomegalovirus induced DNA polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine. J. Virol. 53:1985;776-780.
6. Xiong X., Smith J. L., Kim C., Huang E.-S., Chen M. S. Kinetic analysis of the interaction of cidofovir diphosphate with human cytomegalovirus DNA polymerase. Biochem. Pharmacol. 51:1996;1563-1567.
7. Öberg B. Antiviral effects of phosphonophormate (PFA, foscarnet sodium). Pharmac. Ther. 40:1989;213-285.
8. Mar E.-C., Chiou J.-F., Cheng Y.-C., Huang E.-S. Human cytomegalovirus-induced DNA polymerase and its interaction with the triphosphates of 1-(2′-deoxy-2′-fluoro-β-D. J. Virol. 56:1985;846-851.
9. Nishiyama Y., Maeno K., Yoshida S. Characterization of human cytomegalovirus-induced DNA polymerase and the associated 3′-to-5′ exonuclease. Virology. 124:1983;221-231.
10. Bernad A., Blanco L., Lazaro J. M., Martin G., Salas M. A conserved 3′-5′ exonuclease active site in procaryotic and eucaryotic DNA polymerases. Cell. 59:1989;219-228.
11. Ertl P. F., Powell K. L. Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein ICP36 expressed in insect cells. J. Virol. 66:1992;4126-4133.
12. Weiland K. L., Oien N. L., Homa F., Wathen M. W. Functional analysis of human cytomegalovirus polymerase accessory protein. Virus Res. 34:1994;191-206.
13. Coen D. Viral DNA polymerases. DNA Replication in Eucaryotic Cells. 1996;Cold Spring Harbor Laboratory Press, Cold Spring Harbor. p. 495-523.
14. Ertl P. F., Thomas M. S., Powell K. L. High level expression of DNA polymerases from herpesviruses. J. Gen. Virol. 72:1991;1729-1734.
15. Pelham H. R. B., Jackson R. J. An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67:1976;247-256.
16. Glass C. A., Pollard K. M. Post-translation isoprenylation of human lamin B in the reticulocyte lysate in vitro translation system. Promega Notes. 26:1990;6-9.
17. Kozak M. Influences of mRNA secondary structure on initiation by eucaryotic ribosomes. Proc. Natl. Acad. Sci. USA. 83:1986;2850-2854.
18. Jackson R. J. Potassium salt influence on the fidelity of mRNA translation initiation in rabbit reticulocyte lysates: Unique features of encephalomyocarditis virus RNA translation. Biochim. Biophys. Acta. 1088:1991;345-358.
19. Jobling S. A., Gehrke L. Enhanced translation of chimaeric messenger RNAs containing a plant viral untranslated leader sequence. Nature. 325:1987;622-625.
20. Falcone D., Andrews D. W. Both the 5′ untranslated region and the sequences surrounding the start site contribute to efficient initiation of translation in vitro. Mol. Cell. Biol. 11:1991;2656-2664.
21. Dorsky D. I., Crumpacker C. S. Expression of herpes simplex virus type 1 DNA polymerase gene by in vitro translation and effect of gene deletions on activity. J. Virol. 62:1988;3224-3232.
22. Ye L.-B., Huang E.-S. In vitro expression of the human cytomegalovirus DNA polymerase gene: Effects of sequence alterations on enzyme activity. J. Virol. 67:1993;6339-6347.
23. Thompson D., Van Oosbree T., Beckler G., Van Herwynen J. The TNT lysate systems: One step transcription/translation in rabbit reticulocyte lysate. Promega Notes. 35:1992;1-4.
24. Cherrington J. M., Allen S. J., McKee B. H., Chen M. S. Kinetic analysis of the interaction between the diphosphate of (S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, ddCTP, AZTTP, and FIAUTP with human DNA polymerases β and γ Biochem. Pharmacol. 48:1994;1986-1988.
25. Thompson D., Van Oosbree T. TNT lysate coupled transcription/translation: Comparison of the T3, T7 and SP6 systems. Promega Notes. 38:1995;13-14.
26. Jackson R. J. Cytoplasmic regulation of mRNA function: Importance of the 3′-untranslated region. Cell. 74:1993;9-14.
27. Tanguay R. L., Gallie D. R. Translation efficiency is regulated by the length of the 3′ untranslated region. Mol. Cell. Biol. 16:1996;146-156.
28. Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eucaryotic rybosomes. Cell. 44:1986;283-292.
29. Crute J. J., Lehman I. R. Herpes simplex 1 DNA polymerase: identification of an intrinsic 5′-3′ exonuclease with ribonuclease H activity. J. Biol. Chem. 264:1989;19266-19270.
30. Hart G. J., Boehme R. E. The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1. FEBS Letts. 305:1992;97-100.