Interaction between human respiratory syncytial virus (RSV) M2-1 and P proteins is required for reconstitution of M2-1-dependent RSV minigenome activity

Journal of Virology

Volumn 77, Issue 19, 2003, Pages 10670-10676

  Mason, Stephen W ^a   Aberg, Erika ^a   Lawetz, Carol ^a   DeLong, Rachel ^a   Whitehead, Paul ^a   Liuzzi, Michel ^a  

^a BOEHRINGER INGELHEIM CANADA LTD   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

RNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS M2 1 PROTEIN; VIRUS P PROTEIN; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ENZYME SUBUNIT; GENE FUNCTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; REPORTER GENE; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; VIRUS GENOME; VIRUS MUTATION; VIRUS REPLICATION;

CHROMATOGRAPHY, AFFINITY; GENOME, VIRAL; HUMANS; PHOSPHORYLATION; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; TRANSCRIPTION, GENETIC; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 0141632677     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.77.19.10670-10676.2003     Document Type: Article

References (33)

1. Asenjo, A., and N. Villanueva. 2000. Regulated but not constitutive human respiratory syncytial virus (HRSV) P protein phosphorylation is essential for oligomerization. FEBS Lett. 467:279-284.
2. Bitko, V., and S. Barik. 2001. Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses. BMC Microbiol. 1:34.
3. Cartee, T. L., and G. W. Wertz. 2001. Respiratory syncytial virus M2-1 protein requires phosphorylation for efficient function and binds viral RNA during infection. J. Virol. 75:12188-12197.
4. Collins, P. L., R. M. Chanock, and B. R. Murphy. 2001. Respiratory syncytial virus, p. 1443-1485. In D. M. Knipe and P. M. Howley (ed.), Fields virology. Lippincott Williams & Wilkins, New York, N.Y.
5. Collins, P. L., E. Camargo, and M. G. Hill. 1999. Support plasmids and support proteins required for recovery of recombinant respiratory syncytial virus. Virology 259:251-255.
6. Collins, P. L., M. G. Hill, J. Cristina, and H. Grosfeld. 1996. Transcription elongation factor of respiratory syncytial virus, a nonsegmented negative-strand RNA virus. Proc. Natl. Acad. Sci. USA 93:81-85.
7. Collins, P. L., M. A. Mink, and D. S. Stec. 1991. Rescue of synthetic analogs of respiratory syncytial virus genomic RNA and effect of truncations and mutations on the expression of a foreign reporter gene. Proc. Natl. Acad. Sci. USA 88:9663-9667.
8. Cuesta, I., X. Geng, A. Asenjo, and N. Villanueva. 2000. Structural phosphoprotein M2-1 of the human respiratory syncytial virus is an RNA binding protein. J. Virol. 74:9858-9867.
9. 215 and is without effect. J. Virol. 73:8384-8392.
10. Fearns, R., and P. L. Collins. 1999. Role of the M2-1 transcription antitermination protein of respiratory syncytial virus in sequential transcription. J. Virol. 73:5852-5864.
11. Fearns, R., M. E. Peeples, and P. L. Collins. 2002. Mapping the transcription and replication promoters of respiratory syncytial virus. J. Virol. 76:1663-1672.
12. Formosa, T., J. Barry, B. M. Alberts, and J. Greenblatt. 1991. Using protein affinity chromatography to probe structure of protein machines. Methods Enzymol. 208:24-45.
13. Garcia, J., B. Garcia-Barreno, J. Martinez, and J. A. Melero. 1993. Mapping of monoclonal antibody epitopes of the human respiratory syncytial virus P protein. Virology 195:239-242.
14. Garcia, J., B. Garcia-Barreno, A. Vivo, and J. A. Melero. 1993. Cytoplasmic inclusions of respiratory syncytial virus-infected cells: formation of inclusion bodies in transfected cells that coexpress the nucleoprotein, the phosphoprotein, and the 22K protein. Virology 195:243-247.
15. Garcia-Barreno, B., T. Delgado, and J. A. Melero. 1996. Identification of protein regions involved in the interaction of human respiratory syncytial virus phosphoprotein and nucleoprotein: significance for nucleocapsid assembly and formation of cytoplasmic inclusions. J. Virol. 70:801-808.
16. Greenblatt, J., J. R. Nodwell, and S. W. Mason. 1993. Transcriptional antitermination. Nature 364:401-406.
17. Grosfeld, H., M. G. Hill, and P. L. Collins. 1995. RNA replication by respiratory syncytial virus (RSV) is directed by the N, P, and L proteins: transcription also occurs under these conditions but requires RSV superinfection for efficient synthesis of full-length mRNA. J. Virol. 69:5677-5686.
18. Hardy, P. W., S. B. Harmon, and G. W. Wertz. 1999. Diverse gene junctions of respiratory syncytial virus modulate the efficiency of transcription termination and respond differently to M2-mediated antitermination. J. Virol. 73:170-176.
19. Hardy, R. W., and G. W. Wertz. 1998. The product of the respiratory syncytial virus M2 gene ORF1 enhances readthrough of intergenic junctions during viral transcription. J. Virol. 72:520-526.
20. 1 motif of the respiratory syncytial virus M2-1 protein is essential for protein function. J. Virol. 74:5880-5885.
21. Hengst, U., and P. Kiefer. 2000. Domains of human respiratory syncytial virus P protein essential for homodimerization and for binding to N and NS1 protein. Virus Genes 20:221-225.
22. Khattar, S. K., A. S. Yunus, P. L. Collins, and S. K. Samal. 2001. Deletion and substitution analysis defines regions and residues within the phosphoprotein of bovine respiratory syncytial virus that affect transcription, RNA replication, and interaction with the nucleoprotein. Virology 285:253-269.
23. Khattar, S. K., A. S. Yunus, and S. K. Samal. 2001. Mapping the domains on the phosphoprotein of bovine respiratory syncytial virus required for N-P and P-L interactions using a minigenome system. J. Gen. Virol. 82:775-779.
24. Kobor, M. S., and J. Greenblatt. 2002. Regulation of transcription elongation by phosphorylation. Biochim. Biophys. Acta 1577:261-275.
25. Kuo, L., H. Grosfeld, J. Cristina, M. G. Hill, and P. L. Collins. 1996. Effects of mutations in the gene-start and gene-end sequence motifs on transcription of monocistronic and dicistronic minigenomes of respiratory syncytial virus. J. Virol. 70:6892-6901.
26. Lambert, D. M., J. Hambor, M. Diebold, and B. Galinski. 1988. Kinetics of synthesis and phosphorylation of respiratory syncytial virus polypeptides. J. Gen. Virol. 69:313-323.
27. Lee, T. I., and R. A. Young. 2000. Transcription of eukaryotic protein-coding genes. Annu. Rev. Genet. 34:77-137.
28. Mazumder, B., G. Adhikary, and S. Barik. 1994. Bacterial expression of human respiratory syncytial viral phosphoprotein P and identification of Ser237 as the site of phosphorylation by cellular casein kinase II. Virology 205:93-103.
29. Slack, M. S., and A. J. Easton. 1998. Characterization of the interaction of the human respiratory syncytial virus phosphoprotein and nucleocapsid protein using the two-hybrid system. Virus Res. 55:167-176.
30. Sutherland, K. A., P. L. Collins, and M. E. Peeples. 2001. Synergistic effects of gene-end signal mutations and the M2-1 protein on transcription termination by respiratory syncytial virus. Virology 288:295-307.
31. Tang, R. S., N. Nguyen, X. Cheng, and H. Jin. 2001. Requirement of cysteines and length of the human respiratory syncytial virus M2-1 protein for protein function and virus viability. J. Virol. 75:11328-11335.
32. Weisberg, R. A., and M. E. Gottesman. 1999. Processive antitermination. J. Bacteriol. 181:359-367.
33. Yu, Q., R. W. Hardy, and G. W. Wertz. 1995. Functional cDNA clones of the human respiratory syncytial (RS) virus N, P, and L proteins support replication of RS virus genomic RNA analogs and define minimal trans-acting requirements for RNA replication. J. Virol. 69:2412-2419.