Kinetics of experimental infection of sheep with Ehrlichia ruminantium cultivated in tick and mammalian cell lines

Experimental and Applied Acarology

Volumn 28, Issue 1-4, 2002, Pages 187-193

  Postigo, Milagros ^a   Bell Sakyi, Lesley ^a   Paxton, Edith ^a   Sumption, Keith ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Ehrlichia ruminantium; Heartwater; Real time PCR; Rickettsaemia; Tick cell lines

Indexed keywords

SHEEP; TICK;

ANIMALS; BRAIN; CATTLE; CELLS, CULTURED; DNA, BACTERIAL; EHRLICHIA RUMINANTIUM; HEARTWATER DISEASE; IXODIDAE; KINETICS; POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES; TICK-BORNE DISEASES;

ACARI; EHRLICHIA; EHRLICHIA RUMINANTIUM; MAMMALIA; MELOPHAGUS OVINUS; OVIS ARIES;

EID: 0141457762     PISSN: 01688162     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1025390215007     Document Type: Conference Paper

References (10)

1. Bekker, C.P.J., Bell-Sakyi, L., Paxton, E.A., Martinez, D., Bensaid, A. and Jongejan, F. 2002. Transcriptional analysis of the major antigenic protein 1 multigene family of Cowdria ruminantium. Gene 285: 193-201.
2. Bell-Sakyi, L. 1991. Continuous cell lines from the tick Hyalomma anatolicum anatolicum. J. Parasitol. 77: 1006-1008.
3. Bell-Sakyi, L., Paxton, E.A., Munderloh, U.G. and Sumption, K.J. 2000a. Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line. J. Clin. Microbiol. 8: 1238-1240.
4. Bell-Sakyi, L., Paxton, E.A., Munderloh, U.G. and Sumption, K.J. 2000b. Morphology of Cowdria ruminantium grown in two tick cell lines. In: Proceedings of the 3rd International Conference "Tick and Tick-borne Pathogens: Into the 21st Century", M. Kazimirova, M. Labuda and P.A. Nuttall (eds), pp. 131-137. Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia.
5. Camus, E., Barre, N., Martinez, D. and Uilenberg, G. 1996. Heartwater (Cowdriosis) a Review, 2nd edn., 177 pp. O.I.E.
6. Mahan, S.M., Allsopp, B.A., Kocan, K.M., Palmer, G.H. and Jongejan, F. 1999. Vaccine strategies for Cowdria ruminantium infections and their application to other ehrlichial infections. Parasitol. Today 15: 290-294.
7. Munderloh, U.G. and Kurtti, T.J. 1989. Formulation of medium for tick cell culture. Exp. Appl. Acarol. 7: 219-229.
8. Mutunga, M., Preston, P.M. and Sumption, K.J. 1998. Nitric oxide is produced by Cowdria ruminantium-infected bovine pulmonary endothelial cells in vitro and is stimulated by gamma interferon. Infect. Immun. 66: 2115-2121.
9. Pusterla, N., Huder, J.B., Leutenegger, C.M., Braun, U., Madigan, J.E. and Lutz, H. 1999a. Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J. Clin. Microbiol. 37: 1329-1331.
10. Pusterla, N., Leutenegger, C.M., Chae, J., Lutz, H., Kimsey, R.B., Dumler, J.S. and Madigan, J.E. 1999b. Quantitative evaluation of ehrlichial burden in horses after experimental transmission of human granulocytic ehrlichia agent by intravenous inoculation with infected leukocytes and by infected ticks. J. Clin. Microbiol. 37: 4042-4044.