Escherichia coli cystathionine γ-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions

Biochemistry

Volumn 42, Issue 38, 2003, Pages 11297-11306

  Aitken, Susan M ^a   Kim, Daniel H ^a   Kirsch, Jack F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELIMINATION REACTIONS;

CATALYSIS; ESCHERICHIA COLI; HYDROLYSIS; PH; REACTION KINETICS; SUBSTITUTION REACTIONS;

ENZYME KINETICS;

BACTERIAL ENZYME; CYSTATHIONINE GAMMA SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; ELIMINATION REACTION; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PH; PRIORITY JOURNAL; REACTION ANALYSIS; STRUCTURE ANALYSIS;

CARBON-OXYGEN LYASES; CATALYSIS; CYSTEINE; ESCHERICHIA COLI; HOMOSERINE; HYDROGEN-ION CONCENTRATION; KINETICS; L-LACTATE DEHYDROGENASE; LYASES; METHIONINE; NAD; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; SPECTROPHOTOMETRY; SUCCINIC ACID; TITRIMETRY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0141456354     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035107o     Document Type: Article

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