Characterization of the interactions between Asp141 and Phe 236 in the Mn2+-L-malate binding of pigeon liver malic enzyme

Biochemical Journal

Volumn 374, Issue 3, 2003, Pages 633-637

  Chen, Yen I ^a   Chen, Yu Hou ^a   Chou, Wei Yuan ^a   Chang, Gu Gang ^b  

^a NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

Binding energy; Coupling energy; Malic enzyme; Metal binding site; Mutation cycle; Pigeon liver

Indexed keywords

CLONING; CRYSTAL STRUCTURE; FLUORESCENCE; MUTAGENESIS; OXIDATION;

LIGANDS;

ENZYME KINETICS;

ASPARAGINE; ENZYME; LIGAND; LIVER ENZYME; MALATE DEHYDROGENASE (DECARBOXYLATING); MALIC ACID; MANGANESE; PHENYLALANINE; ASPARTIC ACID; MALATE DEHYDROGENASE; MALATE DEHYDROGENASE (OXALOACETATE DECARBOXYLATING) (NADP+); MALATE DEHYDROGENASE (OXALOACETATE-DECARBOXYLATING) (NADP+); MALIC ACID DERIVATIVE; METAL; RECOMBINANT PROTEIN;

ARTICLE; BINDING AFFINITY; CHEMICAL ANALYSIS; COMPARATIVE STUDY; DISSOCIATION CONSTANT; FLUORESCENCE; FUNCTIONAL ASSESSMENT; KINETICS; METAL BINDING; MUTANT; PIGEON; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN QUATERNARY STRUCTURE; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; WILD TYPE; ANIMAL; BINDING SITE; CHEMISTRY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LIVER; PIGEONS AND DOVES; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

COLUMBA;

ANIMALS; ASPARTIC ACID; BINDING SITES; COLUMBIDAE; KINETICS; LIVER; MALATE DEHYDROGENASE; MALATES; MANGANESE; METALS; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; PROTEIN BINDING; RECOMBINANT PROTEINS; THERMODYNAMICS;

EID: 0141456157     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20030268     Document Type: Article

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