A strategy for the sequential patterning of proteins: Catalytically active multiprotein nanofabrication

Nano Letters

Volumn 3, Issue 6, 2003, Pages 691-694

  Jang, Chang Hyun ^a   Stevens, Benjamin D ^b   Phillips, Ryan ^b   Calter, Michael A ^b   Ducker, William A ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINESTERASE; ENZYME; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME SYNTHESIS; FILM; PROTEIN IMMOBILIZATION;

EID: 0141452007     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl0259636     Document Type: Article

References (16)

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10. The threshold force for nanografting is a function of the tip radius, the type of the thiol, and the roughness of the gold surface. Therefore, the applied force for nanografting was determined for each individual experiment.
11. Our results do not allow us to determine whether this is physisorption or chemisorption by reaction with two ω-alcohols from neighboring EOthiols.
12. This product contains 7-15% active AChE.
13. Jones, M. Organic Chemistry; W. W. Norton & Company, Inc.: New York, 1997; p 1092.
14. The molar mass of AChE is about 70 kDa per catalytic site (Rosenberry, T. L. Adv. Enzymol. Relat. Areas Mol. Biol. 1975, 43, 103-218), and the molar mass of insulin is 5.8 kDa (Smith, L. F. Am. J. Med. 1966, 40, 662-666).
15. The molar mass of AChE is about 70 kDa per catalytic site (Rosenberry, T. L. Adv. Enzymol. Relat. Areas Mol. Biol. 1975, 43, 103-218), and the molar mass of insulin is 5.8 kDa (Smith, L. F. Am. J. Med. 1966, 40, 662-666).
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