Sandwich ELISAs for quantification of Xenopus laevis vitellogenin and albumin and their application to measurement of estradiol-17β effects on whole animals and primary-cultured hepatocytes

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 135, Issue 3, 2003, Pages 305-313

  Mitsui, Naoko ^a   Tooi, Osamu ^a   Kawahara, Akira ^b  

^a Towa Kagaku Co Ltd   (Japan)

^b HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Albumin; ELISA; Estradiol 17 ; Hepatocyte; Plasma assay; Primary culture; Vitellogenin; Xenopus laevis

Indexed keywords

ALBUMIN; ESTRADIOL; VITELLOGENIN;

AGE; ANIMAL; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BODY MASS; CELL DENSITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; DILUTION; DRUG POTENCY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESTROGEN ACTIVITY; EXPOSURE; IMMERSION; IN VITRO STUDY; LIVER CELL CULTURE; MALE; MATURITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN DETERMINATION; PROTEIN INDUCTION; PROTEIN SECRETION; QUANTITATIVE ANALYSIS; SCREENING TEST; XENOPUS LAEVIS;

ANIMALIA; XENOPUS LAEVIS;

EID: 0043092295     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1532-0456(03)00116-9     Document Type: Article

References (28)

1. Bon E., Barbe U., Rodriguez J.N., Cuisset B., Pelissero C., Sumpter J.P., et al. Plasma vitellogenin levels during the annual reproductive cycle of the female rainbow trout (Oncorhynchus mykiss): establishment and validation of an ELISA. Comp. Biochem. Physiol. B. 117:1997;75-84.
2. Clemens M.J., Lofthouse R., Tata J.R. Sequential changes in the protein synthetic activity of male Xenopus laevis liver following induction on egg-yolk proteins by estradiol-17β J. Biol. Chem. 250:1975;2213-2218.
3. Fenske M., van Aerler R., Brack S., Tyler C.R., Segner H. Development and validation of a homologous zebrafish (Danio rerio Hamilton-Buchanan) and its application for sudies on estrogenic chemicals. Comp. Biochem. Physiol. C. 129:2001;217-232.
4. Foran C.M., Bennett E.R., Benson W.H. Exposure to environmentally relevant concentrations of different non-ylphenol formulations in Japanese medaka. Mar. Environ. Res. 50:2000;135-139.
5. Fukuda H., Haga A., Fujita T., Hiramatsu N., Sulivan V.C., Hara A. Development and validation of chemiluminescent immunoassay for vitellogenin in five Salmonid species. Comp. Biochem. Physiol. A. 130:2001;163-170.
6. Holbech H., Andersen L., Petersen G.I., Korsgaard B., Pedersen K.L., Bjerregaard P. Development of an ELISA for vitellogenin in whole body homogenate of zebrafish (Danio rerio). Comp. Biochem. Physiol. C. 130:2001;119-131.
7. Johnsen H.K., Tweiten H., Willassen N.P., Arnsen A.M. Arctic charr (Salvelius alpinus) vitellogenin: development and validation of an enzyme-linked immunosorbent assay. Comp. Biochem. Physiol. B. 124:1999;355-362.
8. Kawahara A., Sato K., Amano M. Quantitative analysis of protein synthesis altered by estrogen in cultured Xenopus liver parenchymal cell. Dev. Growth Differ. 23:1981;599-611.
9. Kawahara A., Sato K., Amano M. Regulation of protein synthesis by estradiol-17β, dexamethasone, and insulin in primary cultured Xenopus liver parenchymal cells. Exptl. Cell Res. 148:1983;423-436.
10. Kordes C., Rieber E.P., Gutzeit H.O. An in vitro vitellogenin bioassay for oestrogenic substances in the medaka (Oryzias latipes). Aquat. Toxicol. 58:2002;151-164.
11. Laemmli U.K. Cleavage of the structural proteins during assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
12. Lutz I., Kloas W. Amphibians as a model to study endocrine disruptors: I. Environmental pollution and estrogen receptor binding. Sci. Total Environ. 225:1999;49-57.
13. Monteverdi G.H., Di Giulio R.T. An enzyme-linked immunosorbent assay for estrogenicity using primary hepatocyte cultures from the channel catfish (Ictalurus punctatus). Arch. Environ. Contam. Toxicol. 37:1999;62-69.
14. Mosconi G., Carnevali O., Carletta R., Nabissi M., Polzonetti-Maggni A.M. Gilthead seabream (Sparus auratta) vitellogenin: purification, partial characterization, and validation of an enzyme-linked immunosorbent assay (ELISA). Gen. Comp. Endorinol. 110:1998;252-261.
15. Nishi K., Chikae M., Hatano Y., Mizukami H., Yamashita M., Sakakibara R., et al. Development and application of a monoclonal antibody-based sandwich ELISA for quantification of Japanese medaka (Oryzias latipes) vitellogenin. Comp. Biochem. Physiol. C. 132:2002;161-169.
16. Parks L.G., Cheek A.O., Denslow N.D., Heppell S.A., McLachlan J.A., LeBlanc G.A., et al. Fathead minnow (Pimephales promelas) vitellogenin: purification, characterization and quantitative immunoassay for the detection of estrogenic compounds. Comp. Biochem. Physiol. C. 123:1999;113-125.
17. Rankouhi T.R., van Holsteijn I., Letcher R., Giesy J.P., van Den Berg M. Effects of primary exposure to environmental and natural estrogens on vitellogenin production in carp (Cyprinus carpio) hepatocytes. Toxicol. Sci. 67:2002;75-80.
18. Smeets J.M., van Holsteijn I., Giesy J.P., Seinen W., van den Berg M. Estrogenic potencies of several environmental pollutants, as determined by vitellogenin induction in a carp hepatocyte assay. Toxicol. Sci. 50:1999;206-213.
19. Sumpter J.P., Jobling S. Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. Environ. Health Perspect. 103:1995;173-178.
20. Tata J.R. The expression of the vitellogenin gene. Cell. 9:1976;1-14.
21. Tata J.R. Regulation of expression of Xenopus vitellogenin genes. Browder L.W. Developmental Biology. 5:1988;241-265 Plenum Publishing Corporation, New York.
22. Tijissen P., Kurstak E. Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassay. Anal. Biochem. 1984;136451-136457.
23. Tyler C.R., van Aerle R., Hutchinson T.H., Maddix S., Trip H. An in vivo testing system for endocrine disruptors in fish early life stages using induction of vitellogenin. Environ. Toxicol. Chem. 18:1999;337-347.
24. van Wyk J.H., Pool E.J., Leslie A.J. The effect of anti-androgenic and estrogenic disrupting contaminants on breeding gland (nuptial pad) morphology, plasma testosterone levels, and plasma vitellogenin levels in male Xenopus laevis (African clawed frog). Arch. Environ. Contam. Toxicol. 44:2003;247-256.
25. Wahli W., David I.B., Ryffel G.U., Weber R. Vitellogenesis and the vitellogenin gene family. Science. 212:1981;298-304.
26. Wallace R.A. Resolution and isolation of avian and amphibian yolk-granule proteins using TEAE-cellulose. Anal. Biochem. 11:1965;297-311.
27. Westley B., Knowland J. An estrogen receptor from Xenopus laevis liver possibly connected with vitellogenin synthesis. Cell. 15:1978;367-374.
28. Westley B., Knowland J. Estrogen causes a rapid, large and prolonged rise in the level of nuclear estrogen receptor in Xenopus laevis liver. Biochem. Biophys. Res. Commun. 88:1979;1167-1172.