Comparison of bacterial enriched-broth culture, enzyme linked immunosorbent assay, and broth culture-polymerase chain reaction techniques for identifying asymptomatic infections with Salmonella in swine

Canadian Journal of Veterinary Research

Volumn 67, Issue 3, 2003, Pages 219-224

  Sibley, Jennifer ^a   Yue, Binbin ^a   Huang, Fei ^a   Harding, John ^b   Kingdon, Jill ^b   Chirino Trejo, Manuel ^a   Appleyard, Greg D ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b Harding Swine Vet Service Inc   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ANIMAL EXPERIMENT; ANIMAL SALMONELLOSIS; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; CULTURE MEDIUM; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA EXTRACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE DISEASE;

ANIMALS; ANTIBODIES, BACTERIAL; CULTURE MEDIA; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FECES; POLYMERASE CHAIN REACTION; RANDOM ALLOCATION; REPRODUCIBILITY OF RESULTS; SALMONELLA; SALMONELLA INFECTIONS, ANIMAL; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

ANIMALIA; BACTERIA (MICROORGANISMS); SALMONELLA; SUS SCROFA;

EID: 0042922770     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Wood RL, Pospischil A, Rose R. Distribution of persistent Salmonella typhimurium infection in internal organs of swine. Am J Vet Res 1989;50:1015-1021.
2. Hurd HS, Schlosser W, Ebel ED. The effect of intermittent shedding on prevalence estimation in populations. Proc 3rd Intl Symp Epidemiology and Control of Salmonella in Pork 1999:57-58.
3. Gray JT, Stabel TJ, Fedorka-Cray PJ. Effect of dose on the immune response and persistence of Salmonella choleraesuis infection in swine. Am J Vet Res 1996;57:313-319.
4. Bennet AR, Greenwood D, Tennant C, Banks JG, Betts RP. Rapid and definitive detection of Salmonella in foods by PCR. Lett Appl Micro 1998;26:437-441.
5. Bailey JS. Detection of Salmonella cells within 24 to 36 hours in poultry samples with polymerase chain reaction BAX system. J Food Prot 1998;61:792-795.
6. Gouws PA, Visser MA, Brozel VS. A polymerase chain reaction procedure for the detection of Salmonella spp. within 24 hours. J Food Prot 1998;61:1039-1042.
7. Davies PR, Funk JA, Nichols MG, et al. Effects of some methodological factors on detection of Salmonella in swine feces. Proc 3rd Intl Symp Epidemiology and Control of Salmonella in Pork 1999:30-32.
8. O'Carroll JM, Davies PR, Correa MT, Slenning BD. Effects of sample storage and delayed secondary enrichment on detection of Salmonella spp in swine feces. Am J Vet Res 1999;60:359-362.
9. Clarke RC, Gyles CL. Salmonella. In: Gyles CL, Thoen CO, eds. Pathogenesis of Bacterial Infections in Animals. 2nd ed. Ames: Iowa State University Press, 1993:133-153.
10. World Health Organization. Salmonellosis control: the role of animal and product hygiene: a report of a WHO committee. WHO Technical Report Series No. 744. 1988. World Health Organization, Geneva, Switzerland.
11. Chirino-Trejo JM. A more sensitive procedure for the detection of Salmonella carriers in swine. Proc Western Can Assoc Swine Pract 1999:50-53.
12. Cohen ND, Martin LJ, Simpson RB, Wallis DE, Neibergs HL. Comparison of polymerase chain reaction and microbiological culture for detection of salmonellae in equine feces and environmental samples. Am J Vet Res 1996;57:780-786.
13. Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press. 1989.
14. Rahn K, De-Grandis SA, Clarke RC, et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 1992;6:271-279.
15. Cohen ND, McGruder ED, Neibergs HL, Behle RW, Wallis DE, Hargis BM. Detection of Salmonella enteritidis in feces from poultry using booster polymerase chain reaction and oligonucleotide primers specific for all members of the genus Salmonella. Poult Sci 1994;73:354-357.
16. Cohen ND, Wallis DE, Neibergs HL, Hargis BM. Detection of Salmonella enteritidis in equine feces using the polymerase chain reaction and genus-specific oligonucleotide primers. J Vet Diagn Invest 1995;7:219-222.
17. Pao CC, Yen TS, You JB, Maa JS, Fiss EH, Chang CH. Detection and identification of Mycobacterium tuberculosis by DNA amplification. J Clin Microbiol 1990;28:1877-1880.
18. Frankel G, Riley L, Giron JA, et al. Detection of Shigella in feces using DNA amplification. J Infect Dis 1990;161:1252-1256.
19. Soumet C, Ermel G, Rose V, et al. Identification by a multiplex PCR-based assay of Salmonella typhimurium and Salmonella enteritidis strains from environmental swabs of poultry houses. Lett Appl Microbiol 1999;29:1-6.
20. Thisted-Lamberts S, Ballagi-Pordány A, Nilsson A, Norberg P, Danielsson-Tham ML. A comparison between a PCR method and a conventional culture method for detecting pathogenic Yersinia enterocolitica in food. J Appl Bacteriol 1996;81:303-308.
21. Dickinson JH, Kroll RG, Grant KA. The direct application of the polymerase chain reaction to DNA extracted from foods. Lett Appl Microbiol 1995;20:212-216.
22. Rossen L, Norskov P, Holmstrøm K, Rasmussen OF. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA extraction systems. Intl J Food Microbiol 1992;17:37-43.
23. Wilde J, Eiden J, Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 1990;28:1300-1307.
24. Monteiro L, Bonnemaison D, Vekris A, et al. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J Clin Microbiol 1997;35:995-998.
25. Bager F, Petersen J. Sensitivity and specificity of different methods for the isolation of Salmonella from pigs. Acta Vet Scand 1991;32:473-481.
26. Proux K, Houdayer C, Humbert F, et al. Development of a complete ELISA using Salmonella lipopolysaccharides of various serogroups allowing to detect all infected pigs. Vet Res 2000;31:481-490.
27. Møller K, Ahrens P. Comparison of toxicity neutralization-, ELISA- and PCR tests for typing of Clostridium perfringens and detection of the enterotoxin gene by PCR. Anaerobe 1996;2:103-110.
28. Salanitro JP, Blake IG, Muirhead PA. Isolation and identification of fecal bacteria from adult swine. Appl Environ Microbiol 1977;33:79-84.
29. Robinson IM, Allison MJ, Bucklin JA. Characterization of the cecal bacteria of normal pigs. Appl Environ Microbiol 1981;41:950-955.