Purification, characterization and gene cloning of thermostable O-acetyl-L-homoserine sulfhydrylase forming γ-cyano-α-aminobutyric acid

Journal of Bioscience and Bioengineering

Volumn 96, Issue 1, 2003, Pages 53-58

  Omura, Hironori ^a,b   Ikemoto, Masahiro ^b   Kobayashi, Michihiko ^c   Shimizu, Sakayu ^c   Yoshida, Toyokazu ^a   Nagasawa, Toru ^a  

^a GIFU UNIVERSITY   (Japan)

^b Ikeda Food Research Co Ltd   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Bacillus stearothermophilus CN3; Cyanide ion tolerant bacterium; O acetyl L homoserine sulfhydrylase; Positron emission tomography; cyano aminobutyric acid

Indexed keywords

ENZYMES; ESCHERICHIA COLI; GENES; POSITRON EMISSION TOMOGRAPHY;

GENE ENCODING;

CLONING;

4 AMINOBUTYRIC ACID; BACTERIAL ENZYME; CARBON 11; CYANIDE; GAMMA CYANO ALPHA AMINOBUTYRIC ACID SYNTHASE; GLUTAMIC ACID; HOMOSERINE; O ACETYLHOMOSERINE SULFHYDRYLASE; RECOMBINANT ENZYME; SYNTHETASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CATALYSIS; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GEOBACILLUS STEAROTHERMOPHILUS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; POSITRON EMISSION TOMOGRAPHY; RECOMBINANT PLASMID; THERMOSTABILITY;

BACILLUS STEAROTHERMOPHILUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; PROKARYOTA; VECTORS;

EID: 0042921350     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(03)90096-X     Document Type: Article

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