Insulators to improve expression of a 3′IgH LCR-driven reporter gene in transgenic mouse models

Biochemical and Biophysical Research Communications

Volumn 307, Issue 3, 2003, Pages 466-471

  Guglielmi, Laurence ^a   Le Bert, Marc ^b   Truffinet, Véronique ^a   Cogné, Michel ^a   Denizot, Yves ^a  

^a CNRS   (France)

^b CDTA   (France)

Author keywords

3 LCR; B lymphocytes; GFP; Insulators; Transgenic mice

Indexed keywords

GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN HEAVY CHAIN;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL DIFFERENTIATION; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION; GENE INSERTION; GENE LOCUS; GENE SILENCING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REGULATOR GENE; REPORTER GENE; TRANSGENE; TRANSGENIC MOUSE;

ANIMALIA; INSERTION SEQUENCES; MUS MUSCULUS;

EID: 0042672597     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)01185-9     Document Type: Article

References (24)

1. ′ IgH regulatory region: a complex structure in a search for a function Adv. Immunol. 75:2000;317-345.
2. Madisen L., Groudine M. Identification of a locus control region in the immunoglobulin heavy-chain locus that deregulates c-myc expression in plasmocytoma and Burkitt's lymphoma cells. Genes Dev. 8:1994;2212-2226.
3. ′ locus control region Eur. J. Immunol. 28:1998;3048-3056.
4. ′ regulatory palindrome in expression vectors warrants sure and efficient expression in stable B cell transfectants Gene. 222:1998;279-285.
5. ′ IgH enhancer elements shift synergistic interactions during B cell development J. Immunol. 160:1998;4896-4903.
6. ′ HS1-4 directs correct spacial position-independent expression of a linked transgene to B lineage cells J. Immunol. 163:1999;4637-4641.
7. West A.G., Gaszner M., Felsenfeld G. Insulators: many functions, many mechanisms. Genes Dev. 16:2002;271-288.
8. Chung J.H., Bell A.C., Felsenfeld G. Characterization of the chicken β-globin insulator. Proc. Natl. Acad. Sci. USA. 94:1997;575-580.
9. Gagneten S., Le Y., Miller J., Sauer B. Brief expression of a GFPcre fusion gene in embryonic stem cells allows rapid retrieval of site specific genomic deletions. Nucleic Acid Res. 25:1997;3326-3331.
10. ′ of the immunoglobulin heavy-chain locus Nature. 344:1990;165-168.
11. ′ enhancer close to the α constant region Mol. Cell Biol. 13:1993;1547-1553.
12. Mills F.C., Harindranath N., Mitchell M., Max E.E. Enhancer complexes located downstream of both human immunoglobulin Cα genes. J. Exp. Med. 186:1997;845-858.
13. ′ IgH locus elements that effect long-distance regulation of class switch recombination Immunity. 15:2001;187-199.
14. Guglielmi L., Le Bert M., Cogné M., Denizot Y. Effect of the Eμ, IgH enhancer on expression of a GFP reporter gene in transfected B cells and transgenic mice. Immunol. Lett. 86:2003;77-83.
15. Mombaerts P., Iacomini J., Johnson R.S., Herrup K., Tonegawa S., Papaioannou V.E. RAG-1 deficient mice have no mature and T lymphocytes. Cell. 68:1992;869-877.
16. Pikaart M.J., Recillas-Targa F., Felsenfeld G. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Gene Dev. 12:1998;2852-2862.
17. Bell A.C., Felsenfeld G. Stopped at the border: boundaries and insulators. Curr. Opin. Genet. Dev. 9:1999;191-198.
18. Okada S., Nakauchi H., Nagayoshi K., Nishikawa S., Nishikawa S.I., Miura Y., Suda T. Enrichment and characterization of murine hematopoietic stem cells that express c-kit molecule. Blood. 78:1991;1706-1712.
19. hi cells coincides with commitment to the B cell lineage. Eur. J. Immunol. 28:1998;3850-3856.
20. Moore T., Huang S., Terstappen L.W., Bennett M., Kumar V. Expression of CD43 on murine and human pluripotent hematopoietic stem cells. J. Immunol. 153:1994;4978-4987.
21. Payne K.J., Medina K.L., Kincade P.W. Loss of c-kit accompanies B-lineage commitment and acquisition of CD45R by most murine B-lymphocyte precursors. Blood. 94:1999;713-723.
22. Tudor K.S., Payne K.J., Yamashita Y., Kincade P.W. Functional assessment of precursors from murine bone marrow suggests a sequence of early B lineage differentiation events. Immunity. 12:2000;335-345.
23. ′ HS4 insulators function to reduce variability in transgenic founder mice Biochem. Biophys. Res. Commun. 273:2000;1015-1018.
24. Pantano T., Jolivet G., Prince S., Menck LeBourhis C., Maeder C., Viglietta C., Rival S., Houdebine L.M. Effect of the rabbit αs1-casein gene distal enhancer on the expression of a reporter gene in vitro and in vivo. Biochem. Biophys. Res. Commun. 290:2002;53-61.