Double-Glycosylated Lysozyme at Positions 19 and 49 Constructed by Genetic Modification and its Surface Functional Properties

Journal of Agricultural and Food Chemistry

Volumn 46, Issue 6, 1998, Pages 2433-2438

  Shu, Yuuei ^a   Maki, Shigeki ^a   Nakamura, Soichiro ^b   Kato, Akio ^a  

^a YAMAGUCHI UNIVERSITY   (Japan)

^b UBE NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Double glycosylation; Emulsifying properties; Genetic modification; Lysozyme; Polymannosylation; Yeast expression system

Indexed keywords

SACCHAROMYCES CEREVISIAE;

EID: 0042611219     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf970989h     Document Type: Article

References (21)

1. Dubois, M.; Gilles, K. A.; Hamilton, J. K.; Rebers, P. A.; Smith, F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28, 350-354.
2. Imoto, T.; Yagishita, K. A simple activity measurement of lysozyme. Agric. Biol. Chem. 1971, 35, 1154-1156.
3. Itoh, H.; Fukuda, Y.; Murata, K.; Kimura, A. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 1983, 153, 163-168.
4. Kato, A.; Sasaki, Y.; Furuta, R.; Kobayashi, K. Functional protein-polysaccharide conjugate prepared by controlled dry-heating of ovalbumin-dextran mixture. Agric. Biol. Chem. 1990, 54, 107-112.
5. Kato, A.; Mifuru, R.; Matsudomi, N.; Kobayashi, K. Functional casein-polysaccharide conjugate prepared by controlled dry heating. Biosci., Biotechnol., Biochem. 1992a, 56, 567-571.
6. Kato, A.; Tanimoto, S.; Muraki, Y.; Kobayashi, K.; Kumagai, I. Structural and functional properties of hen egg-white lysozyme deamidated by protein engineering. Biosci., Biotechnol., Biochem. 1992b, 56, 1424-1428.
7. Kato, a.; Kameyama. K.; Takagi, T. Molecular weight determination and compositional analysis of dextran-protein conjugates using low-angle laser light scattering technique combined with high-performance gel chromatography. Biochim. Biophys. Acta 1992c, 759, 22-28.
8. Kato, A.; Minaki, K.; Kobayashi, K. Improvement of emulsifying properties of egg white proteins by the attachment of polysaccharide through Maillard reaction in a dry state. J. Agric. Food Chem. 1993, 41, 540-543.
9. Kato, A.; Takasaki, H.; Ban, M. Polymannosylation to asparagine-19 in hen egg white lysozyme in yeast. FBBS Lett. 1994, 355, 76-80.
10. Kumagai, I.; Miura, K. Enhanced bactericidal activity of hen egg-white lysozyme due to conversion of Try62 to other. J. Biochem. (Tokyo) 1989, 105, 946-948.
11. 4. Nature 1970, 227, 680-685.
12. Nakamura, S.; Kato, A.; Kobayashi, K. Bifunctional lysozymegalactomannan conjugate having excellent emulsifying properties and bactericidal effect. J. Agric. Food Chem. 1992, 40, 735-739.
13. Nakamura, S.; Takasaki, H.; Kobayashi, K.; Kato, A. Hyperglycosylation of hen egg white lysozyme in yeast. J. Biol. Chem. 1993a, 268, 12706-12712.
14. Nakamura, S.; Kobayashi, K.; Kato, A. Novel surface functional properties of polymannosyl lysozyme constructed by genetic modification. FEBS Lett. 1993b, 328, 259-262.
15. Parry, R. M.; Chandan, R. C.; Shahani, K. M. A rapid and sensitive assay of muramidase. Proc. Soc. Exp. Biol. Med. 1965, 119, 384-386.
16. Pearce, K. M.; Kinsella, J. E. Emulsifying properties of proteins: evaluation of a turbidimeric technique. J. Agric. Food Chem. 1978, 26, 716-732.
17. Sanger, F.; Nicklen, S.; Coulsaon, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 1977, 74, 5463-5467.
18. Shu, Y.; Sahara, S.; Nakamura, S.; Kato, A. Effects of the length of polysaccharide chains on the functional properties of the Maillard-type lysozyme-polysaccharide conjugate. J. Agric. Food Chem. 1996, 44, 2544-2548.
19. Takasaki, S.; Mizuochi, T.; Kobata, A. Methods in Enzymology; Ginsburg, V., Ed.; Academic Press: New York, 1982; Vol. 83, pp 263-268.
20. Toh-e, A.; Wickner, P. B. Curing of the 2μ DNA plasmid from Saccharomyces cerevisiae. J. Bacteriol. 1981, 145, 1421-1429.
21. Yamamoto, K.; Takegawa, K.; Kumagai, H.; Tochikura, T. Effect of protease digestion on the activity of sugar-depleted enzymes prepared with Endo-β-N-acetylglucosaminidase from flavobaterium sp. Agric. Biol. Chem. 1987, 51, 1481-1487.