Effect of high pressure and reversed micelles on the fluorescent proteins

Biochimica et Biophysica Acta - General Subjects

Volumn 1622, Issue 3, 2003, Pages 192-195

  Verkhusha, Vladislav V ^a   Pozhitkov, Alexander E ^b   Smirnov, Sergey A ^c   Borst, Jan Willem ^d   Van Hoek, Arie ^d   Klyachko, Natalya L ^c   Levashov, Andrey V ^c   Visser, Antonie J W G ^d  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF COLOGNE   (Germany)

^c MOSCOW STATE UNIVERSITY   (Russian Federation)

^d WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Chemical potential; Chromophore protonation; Reaction volume; Thermodynamics

Indexed keywords

DOCUSATE SODIUM; GREEN FLUORESCENT PROTEIN; MUTANT PROTEIN; RED FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN;

ARTICLE; CHROMATOPHORE; HYDROSTATIC PRESSURE; HYPERBARISM; MATHEMATICAL COMPUTING; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN STABILITY; PROTON TRANSPORT; QUANTITATIVE ANALYSIS; REVERSE MICELLE; THERMODYNAMICS;

EID: 0042591403     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4165(03)00140-5     Document Type: Article

References (14)

1. Tsien R.Y. The green fluorescent protein. Ann. Rev. Biochem. 67:1998;509-544.
2. Ormö M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., Remington S.J. Crystal structure of the Aequorea victoria green fluorescent protein. Science. 273:1996;1392-1395.
3. Wachter R.M., Elsliger M.A., Kallio K., Hanson G.T., Remington S.J. Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure. 6:1998;1267-1277.
4. Bokman S.H., Ward W.W. Renaturation of Aequorea green fluorescent protein. Biochem. Biophys. Res. Commun. 101:1981;1372-1380.
5. Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
6. Ward W.W., Prentice H.J., Roth A.F., Cody C.W., Reeves S.C. Spectral pertrubations of the Aequorea green fluorescent protein. Photochem. Photobiol. 35:1982;803-809.
7. Matz M.V., Fradkov A.F., Labas Y.A., Savitsky A.P., Zaraisky A.G., Markelov M.L., Lukyanov S.A. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17:1999;969-973.
8. Verkhusha V.V., Otsuna H., Awasaki T., Oda H., Tsukita S., Ito K. An enhanced mutant of red fluorescent protein DsRed for double labeling and developmental timer of neural fiber bundle formation. J. Biol. Chem. 276:2001;29621-29624.
9. Wall M.A., Socolich M., Ranganathan R. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat. Struct. Biol. 7:2000;1133-1138.
10. Yarbrough D., Wachter R.M., Kallio K., Matz M.V., Remington S.J. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution. Proc. Natl. Acad. Sci. U. S. A. 98:2001;462-467.
11. Uskova M.A., Borst J.W., Hink M.A., van Hoek A., Schots A., Klyachko N.L., Visser A.J.W.G. Fluorescence dynamics of green fluorescent protein in AOT reversed micelles. Biophys. Chemist. 87:2000;73-84.
12. Greiner W., Neise L., Stoecker H. Thermodynamics and Statistical Mechanics. 1995;Springer-Verlag, New York.
13. Llopis J., McCaffery J.M., Miyawaki A., Farquhar M.G., Tsien R.Y. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. U. S. A. 95:1998;6803-6808.
14. Patterson G., Day R.N., Piston D. Fluorescent protein spectra. J. Cell. Sci. 114:2001;837-838.