Covalent Immobilization of α-Amylase onto Poly(methyl methacrylate-2-hydroxyethyl methacrylate) Microspheres and the Effect of Ca2+ Ions on the Enzyme Activity

Starch/Staerke

Volumn 51, Issue 6, 1999, Pages 211-217

  Tümtürk, Hayrettin ^a   Aksoy, Serpil ^a   Hasirci, Nesrin ^b  

^a GAZI UNIVERSITY   (Turkey)

^b MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0042542783     PISSN: 00389056     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1521-379x(199906)51:6<211::aid-star211>3.3.co;2-q     Document Type: Article

References (26)

1. Handa, T., A. Hirose, T. Akino, K. Watanable, and H. Tsuchlya: Preparation of immobilized α-amylase covalently attached to granular polyacrylonitrile. Biotechnol. Bioeng. 25 (1983), 2957-2967.
2. Arasaratman, V. and K. Balasubramin: Synergistic action of α-amylase and glucoamylase on raw com. Starch/Stärke 30 (1993), 231-233.
3. Ohnishi, M., K. Iwata, T. Tomita, U. Nishikawa, and K. Hirami: Kinetic properties of Rhizopus glucoamylase and Bacillus α-amylase which are immobilized on cellulofine. Starch/Stärke 42 (1990), 486-489.
4. Strumeyer, D. H., A. Constantinides, and J. Freundenberger: Preparation and characterization of α-amylase immobilized on collagen membranes. J. Food Sci. 39 (1974), 498-502.
5. Linko, Y. Y., P. Saarinen, and M. Linko: Starch conversion by soluble and immobilized α-amylase. Biotechnol. Bioeng. 17 (1975), 153-159.
6. Kvétsitadze, S. G. I. and M. S. Dvali: Immobilization of mold and bacterial amylases on silica carriers. Biotechnol. Bioeng. 24 (1982), 1765-1772.
7. Kwashima, K. and K. Umeda: Immobilization of enzymes by radiocopolymerization of monomers. Biotechnol. Bioeng. 17 (1975), 599-602.
8. Yoo, Y. I., Hong, T. and R. T. Hatch: Comparison of α-amylase activities from different assay methods. Biotechnol. Bioeng. 30 (1987), 147-151.
9. Ulbrich, R., A. Schellenberger, and W. Damerav: Studies on the thermal inactivation of immobilized enzymes. Biotechnol. Bioeng. 28 (1986), 511-522.
10. Wykes, J. R., P. Dunnill, and M. D. Lilly: Immobilization of α-amylase by attachment to soluble support materials. Biochim. Biophys. Acta 250 (1971), 522-529.
11. Kennedy, J. F. and M. Paterson: Application of cellulosic fast flow column filters to protein immobilization and recovery. Polym. Int. 30 (1993), 71-81.
12. Ohtsuka, Y., H. Kawaguchi, and T. Yamamoto: Immobilization of α-amylase on polymeric carriers having different structures. J. Appl. Poly. Sci. 29 (1984), 3295-3306.
13. Arica, M. Y., V. Hasirci, and N. G. Alaeddinoglu: Covalent immobilization of α-amylase onto pHEMA microspheres preparation and application to fixed bed reactor. Biomaterials 16 (1995), 761-768.
14. Roig, M. G., A. Slade, and J. F. Kennedy: α-Amylase immobilized on plastic supports: stabilities, pH and temperature profiles and kinetic parameters. Biomat. Art Cells and Immob. Biotech. 21 (1993), 487-525.
15. Bayramoglu, Z., U. Akbulut, and S. Sungur: Immobilization of α-amylase into photographic gelatin by chemical crosslinking. Biomaterials 13 (1992), 704-709.
16. Lee, P. M., K. H. Lee, and S. Y. Siaw: Covalent immobilization of aminoacrylase to alginate for 2-phenylalanine production. J. Chem. Technol. Biotechnol. 58 (1993), 65-70.
17. Demircioglu, H., H. Beyenal, A. Tanyolaç, and N. Hasirci: Immobilization of urease and estimation of effective diffusion coefficients of urea in HEMA and VP copolymer matrices. Polym. Int. 35 (1994), 321-327.
18. Arica, M. Y. and V. Hasirci: Bioreactor applications of glucose oxidase covalently bonded on pHEMA membranes. Biomaterials 14 (1993), 803-808.
19. Tarhan, L.: The effect of substrate diffusion factor on immobilized α-amylase. Starch/Stärke 41 (1989), 315-321.
20. Demirel, G., G. Akovali, A. Tanyolaç, and N. Hasirci: A comparative study of the performance of solid supported and soluble urease for the enzymatic hydrolysis of urea. J. Chem. Technol. Biotechnol. 55 (1992), 319-329.
21. Demircioglu, H., H. Beyenal, A. Tanyolaç, and N. Hasirci: Entrapment of urease in glycol containing polymeric matrices and estimation of effective diffusion coefficients of urea. Polymer 36 (1995), 4091-4096.
22. Pozniak, G., B. Krajewska, and W. Trochimczuk: Urease immobilization on modified polysulphone membrane: preparation and properties. Biomaterials 16 (1995), 129-134.
23. Flor, P. Q. and S. Hayashida: Continuous production of high-glucose syrup by chitin-immobilized amylase. Biotechnol. Bioeng. 25 (1980), 1973-1980.
24. Violet, M. and J. C. Meunier: Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis α-amylase. Biochem. J. 263 (1989), 665-670.
25. Cord, D. S., K. Vanhoof, J. Hu, G. Maesmans, M. Hendrickx, and P. Tabback: Thermostability of soluble and immobilized α-amylase from Bacillus licheniformis. Biotechnol. Bioeng. 40 (1992), 396-402.
26. Aksoy, S., H. Tümtürk, and N. Hasirci: Stability of α-amylase immobilized on poly(methyl methacrylate-acrylic acid) microspheres. J. Biotechnol. 60 (1998), 37-46.