Binding Properties of Bacillus thuringiensis Cry1C δ-Endotoxin to the Midgut Epithelial Membranes of Culex pipiens

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 1, 2003, Pages 94-99

  Kamauchi, Shinya ^a   Yamagiwa, Masashi ^a   Esaki, Motoyuki ^b   Otake, Kanao ^b   Sakai, Hiroshi ^a  

^a OKAYAMA UNIVERSITY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

Bacillus thuringiensis; Cry toxin; Culex pipiens; Membrane binding; Midgut

Indexed keywords

BACILLUS THURINGIENSIS; CULEX PIPIENS; DIPTERA; INSECTA; INVERTEBRATA; LEPIDOPTERA;

DIGOXIGENIN; ENDOTOXIN; LIGAND;

ANIMAL; ARTICLE; BACILLUS THURINGIENSIS; BINDING COMPETITION; CULEX; DRUG EFFECT; EPITHELIUM; IMMUNOHISTOCHEMISTRY; LARVA; MEMBRANE; METABOLISM; MICROVILLUS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING;

ANIMALS; BACILLUS THURINGIENSIS; BINDING, COMPETITIVE; CULEX; DIGOXIGENIN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; EPITHELIUM; IMMUNOHISTOCHEMISTRY; LARVA; LIGANDS; MEMBRANES; MICROVILLI; PROTEIN BINDING;

EID: 0042352393     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.67.94     Document Type: Article

References (37)

1. Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., and Dean, D. H., Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev., 62, 775-806 (1998).
2. Lilley, M., Ruffell, R. N., and Somerville, H. J., Purification of the insecticidal toxin in crystals of Bacillus thuringiensis. J. Gen. Microbiol., 118, 1-11 (1980).
3. Hofmann, C., Vanderbruggen, H., Hofte, H., Van Rie, J., Jansens, S., and Van Mellaert, H., Specificity of Bacillus thuringiensis d-endotoxins is correlated with the presence of high-affinity binding sites in the brush border membrane of target insect midguts. Proc. Natl. Acad. Sci. USA, 85, 7844-7848 (1988).
4. Hofmann, C., Liithy, P., Hiitter, R., and Pliska, V., Binding of the delta endotoxin from Bacillus thurin-giensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae). Eur. J. Biochem., 173, 85-91 (1988).
5. Van Rie, J., Jansens, S., Hofte, H., Degheele, D., and Van Mellaert, H., Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins. Appl. Environ. Microbiol., 56, 1378-1385 (1990).
6. Gazit, E., and Shai, Y., The assembly and organization of the
7. Gazit, E., La Rocca, P., Sansom, M. S. P., and Shai, Y., The structure and organization within the membrane of the helices composing the pore-forming domain of Bacillus thuringiensis d-endotoxin are consistent with an “umbrella-like” structure of the pore. Proc. Natl. Acad. Sci. USA, 95, 12289-12294 (1998).
8. Knowles, B. H., and Ellar, D. J., Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis d-endotoxins with different insect specificity. Biochim. Biophys. Acta, 924, 509-518 (1987).
9. Knight, P. J. K., Crickmore, N., and Ellar, D. J., The receptor for Bacillus thuringiensis CryIA(c) del-ta-endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N. Mol. Microbiol., 11, 429-436 (1994).
10. 86Rb +-K+ efflux in vitro. J. Biol. Chem., 269, 10088-10092 (1994).
11. Vadlamudi, R. K., Ji, T. H., and Bulla, L. A., Jr., A specific binding protein from Manduca sexta for the insecticidal toxin of Bacillus thuringiensis subsp. berliner. J. Biol. Chem., 268, 12334-12340 (1993).
12. Vadlamudi, R. K., Weber, E., Ji, I., Ji, T. H., and Bulla, L. A., Jr., Cloning and expression of a receptor for an insecticidal toxin of Bacillus thuringiensis. J. Biol. Chem., 270, 5490-5494 (1995).
13. Nagamatsu, Y., Toda, S., Yamaguchi, F., Ogo, M., Kogure, M., Nakamura, M., Shibata, Y., and Kat-sumoto, T., Identification of Bombyx mori midgut receptor for Bacillus thuringiensis insecticidal CryIA(a) toxin. Biosci. Biotechnol. Biochem., 62, 718-726 (1998).
14. Liang, Y., Patel, S. S., and Dean, D. H., Irreversible binding kinetics of Bacillus thuringiensis CryIA d-en-dotoxins to gypsy moth brush border membrane vesicles is directly correlated to toxicity. J. Biol. Chem., 270, 24719-24724 (1995).
15. Honee, G., van der Salm, T., and Visser, B., Nucleotide sequence of crystal protein gene isolated from B. thuringiensis subspecies entomocidus 60.5 coding for a toxin highly active against Spodoptera species. Nucleic Acids Res., 16, 6240 (1988).
16. Sanchis, V., Lereclus, D., Menou, G., Chaufaux, J., Guo, S., and Lecadet, M.-M., Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active d-endotoxin gene of Bacillus thuringiensis aizawai 7.29. Mol. Microbiol., 3, 229-238 (1989).
17. Schnepf, H. E., Wong, H. C., and Whiteley, H. R., The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence. J. Biol. Chem., 260, 6264-6272 (1985).
18. Knowles, B. H., and Ellar, D. J., Differential specificity of two insecticidal toxins from Bacillus thuringien-sis var. aizawai. Mol. Microbiol., 2, 153-157 (1988).
19. Knowles, B. H., Francis, P. H., and Ellar, D. J., Structurally related Bacillus thuringiensis d-endotox-ins display major differences in insecticidal activity in vivo and in vitro. J. Cell Sci., 84, 221-236 (1986).
20. Visser, B., van der Salm, T., van der Brink, W., and Folkers, G., Genes from Bacillus thuringiensis en-tomocidus 60.5 coding for insect-specific crystal proteins. Mol. Gen. Genet., 212, 219-224 (1988).
21. Smith, G. P., Merrick, J. D., Bone, E. J., and Ellar, D. J., Mosquitocidal activity of the CrylC d-endotox-in from Bacillus thuringiensis subsp. aizawai. Appl. Environ. Microbiol., 62, 680-684 (1996).
22. Luo, K., Lu, Y.-J., and Adang, M. J., A 106-kDa form of aminopeptidase is a receptor for Bacillus thuringiensis CrylC d-endotoxin in the brush border membrane of Manduca sexta. Insect. Biochem. Mol. Biol., 26, 783-791 (1996).
23. Bourgouin, C., Delecluse, A., Ribier, J., Klier, A., and Rapoport, G., A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences. J. Bacteriol., 170, 3575-3583 (1988).
24. Poncet, S., Delecluse, A., Klier, A., and Rapoport, G., Evaluation of synergistic interactions between the CrylVA, CrylVB and CrylVD toxic components of Bacillus thuringiensis subsp. israelensis crystals. J. In-vertebr. Pathol., 66, 131-135 (1995).
25. Delecluse, A., Poncet, S., Klier, A., and Rapoport, G., Expression of cryIVA and cryIVB genes, independently or in combination, in a crystal-negative strain of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol., 59, 3922-3927 (1993).
26. Yamagiwa, M., Esaki, M., Otake, K., Inagaki, M., Komano, T., Amachi, T., and Sakai, H., Activation process of dipteran-specific insecticidal protein produced by Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol., 65, 3464-3469 (1999).
27. Yamagiwa, M., Kamauchi, S., Okegawa, T., Esaki, M., Otake, K., Amachi, T., Komano, T., and Sakai, H., Binding properties of Bacillus thuringiensis Cry4A toxin to the apical microvilli of larval midgut of Culex pipiens. Biosci. Biotechnol. Biochem., 65, 2419-2427 (2001).
28. Masson, L., Prefontaine, G., Peloquin, L., Lau, P. C. K., and Brousseau, R., Comparative analysis of the individual protoxin components in P1 crystals of Bacillus thuringiensis subsp. kurstaki isolates NRD-12 and HD-1. Biochem. J., 269, 507-512 (1990).
29. Nishimoto, T., Yoshisue, H., Ihara, K., Sakai, H., and Komano, T., Functional analysis of block 5, one of the highly conserved amino acid sequences in the 130-kDa CryIVA protein produced by Bacillus thuringiensis subsp. israelensis. FEBS Lett., 348, 249-254 (1994).
30. Silva-Filha, M. H., Nielsen-Leroux, C., and Charles, J.-F., Binding kinetics of Bacillus sphaericus binary toxin to midgut brush-border membranes of Anopheles and Culex sp. mosquito larvae. Eur. J. Biochem., 247, 754-761 (1997).
31. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976).
32. Krieger, I. V., Revina, L. P., Kostina, L. I., Buzdin, A. A., Zalunin, I. A., Chestukhina, G. G., and Stepanov, V. M., Membrane proteins of Aedes aegypti larvae bind toxins Cry4B and Cry11A of Bacillus thuringiensis ssp. israelensis. Biochemistry (Mosc.), 64, 1163-1168 (1999).
33. Kwa, M. S. G., de Maagd, R. A., Stiekema, W. J., Vlak, J. M., and Bosch, D., Toxicity and binding properties of the Bacillus thuringiensis delta-endotox-in Cry1C to cultured insect cells. J. Invertebr. Pathol., 71, 121-127 (1998).
34. de Maagd, R. A., Kwa, M. S. G., van der Klei, H., Yamamoto, T., Schipper, B., Vlak, J. M., Stiekema, W. J., and Bosch, D., Domain III substitution in Bacillus thuringiensis delta-endotoxin CryIA(b) results in superior toxicity for Spodoptera exigua and altered membrane protein recognition. Appl. Environ. Microbiol., 62, 1537-1543 (1996).
35. Morse, R. J., Yamamoto, T., and Stroud, R. M., Structure of Cry2Aa suggests an unexpected receptor binding epitope. Structure (Camb.), 9, 409-417 (2001).
36. Li, J., Carroll, J., and Ellar, D. J., Crystal structure of insecticidal d-endotoxin from Bacillus thuringien-sis at 2.5 A resolution. Nature, 353, 815-821 (1991).
37. Grochulski, P., Masson, L., Borisova, S., Pusztai-Carey, M., Schwartz, J.-L., Brousseau, R., and Cygler, M., Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. J. Mol. Biol., 254, 447-464 (1995).