Specific binding of the regulatory protein ExpG to promoter regions of the galactoglucan biosynthesis gene cluster of Sinorhizobium meliloti - A combined molecular biology and force spectroscopy investigation

Journal of Structural Biology

Volumn 143, Issue 2, 2003, Pages 145-152

  Bartels, Frank Wilco ^a   Baumgarth, Birgit ^a   Anselmetti, Dario ^a   Ros, Robert ^a   Becker, Anke ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

Atomic force microscopy; Galactoglucan; Protein DNA interaction; Regulatory protein; Single molecule force spectroscopy; Sinorhizobium meliloti

Indexed keywords

DNA BINDING PROTEIN; GALACTOGLUCAN; GLUCAN; NITROGEN; POLYSACCHARIDE; PROTEIN EXPG; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

ALFALFA; ARTICLE; ATOMIC FORCE MICROSCOPY; CARBOHYDRATE SYNTHESIS; GENE CLUSTER; GENE EXPRESSION REGULATION; GENE SEQUENCE; GRAM NEGATIVE BACTERIUM; MOLECULAR BIOLOGY; NITROGEN FIXATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN FUNCTION; REGULATORY MECHANISM; SINORHIZOBIUM MELILOTI; SOIL MICROFLORA; SYMBIOSIS; TRANSCRIPTION INITIATION;

BACTERIA (MICROORGANISMS); MEDICAGO SATIVA; NEGIBACTERIA; SINORHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0042335817     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1047-8477(03)00127-8     Document Type: Article

References (46)

1. Alekshun M.N., Levy S.B. The mar regulon: multiple resistance to antibiotics and other toxic chemicals. Trends Microbiol. 7:1999;410-413.
2. Astete S.G., Leigh J.A. mucS, a gene involved in activation of galactoglucan (EPS II) synthesis gene expression in Rhizobium meliloti. Mol. Plant Microbe Interact. 9:1996;395-400.
3. Becker A., Rüberg S., Küster H., Roxlau A.A., Keller M., Ivashina T., Cheng H.P., Walker G.C., Pühler A. The 32-kilobase exp gene cluster of Rhizobium meliloti directing the biosynthesis of galactoglucan: genetic organization and properties of the encoded gene products. J. Bacteriol. 179:1997;1375-1384.
4. Becker J.D., Moreira L.M., Kapp D., Frosch S.C., Pühler A., Perlic A.M. The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. Plant Mol. Biol. 47:2001;749-759.
5. Bertram-Drogatz P.A., Quester I., Becker A., Pühler A. The Sinorhizobium meliloti MucR protein, which is essential for the production of high-molecular-weight succinoglycan exopolysaccharide, binds to short DNA regions upstream of exoH and exoY. Mol. Gen. Genet. 257:1998;433-441.
6. Bertram-Drogatz P.A., Rüberg S., Becker A., Pühler A. The regulatory protein MucR binds to a short DNA region located upstream of the mucR coding region in Rhizobium meliloti. Mol. Gen. Genet. 254:1997;529-538.
7. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
8. Bustamante C., Macosko J.C., Wuite G.J.L. Grabbing the cat by the tail: manipulating molecules one by one. Nat. Rev. Mol. Cell Biol. 1:2000;130-136.
9. Carrion-Vasquez M., Oberhauser A., Fowler S., Marszalek P., Broedel S., Clarke J., Fernandez J. Mechanical and chemical unfolding of a single protein: A comparison. Proc. Natl. Acad. Sci. USA. 96:1999;3694-3699.
10. Casse F., Boucher C., Hulliot J.S., Michel M., Dénarié F. Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. J. Bacteriol. 113:1979;229-242.
11. Cohen S.P., Hachler H., Levy S.B. Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli. J. Bacteriol. 175:1993;1484-1492.
12. Dammer U., Popescu O., Wagner P., Anselmetti D., Güntherodt H.-J., Misevic G.N. Binding strength between cell adhesion proteoglycans measured by atomic force microscopy. Science. 267:1995;1173-1175.
13. De Paris R., Strunz T., Oroszlan K., Güntherodt H.-J., Hegner M. Force spectroscopy and dynamics of the biotin-avidin bond studied by scanning force microscopy. Single Molecules. 1:2000;285-290.
14. Egland P.G., Harwood C.S. BadR, a new MarR family member, regulates anaerobic benzoate degradation by Rhodopseudomonas palustris in concert with AadR, an Fnr family member. J. Bacteriol. 181:1999;2102-2109.
15. Evans E., Ritchie K. Dynamic strength of molecular adhesion bonds. Biophys. J. 72:1997;1541-1555.
16. Florin E.-L., Moy V.T., Gaub H.E. Adhesion forces between individual ligand-receptor pairs. Science. 264:1994;415-417.
17. Fritz J., Katopodis A.G., Kolbinger F., Anselmetti D. Force-mediated kinetics of single P-selectin/ligand complexes observed by atomic force microscopy. Proc. Natl. Acad. Sci. USA. 95:1998;12283-12288.
18. Glazebrook J., Walker G.C. A novel exopolysaccharide can function in place of the calcofluor-binding exopolysaccharide in nodulation of alfalfa by Rhizobium meliloti. Cell. 56:1989;661-672.
19. Gonzalez J.E., Reuhs B.L., Walker G.C. Low molecular weight EPS II of Rhizobium meliloti allows nodule invasion in Medicago sativa. Proc. Natl. Acad. Sci. USA. 93:1996;8636-8641.
20. Gottesman S., Halpern E., Trisler P. Role of sulA and sulB in filamentation by lon mutants of Escherichia coli K-12. J. Bacteriol. 148:1981;265-273.
21. Her G.R., Glazebrook J., Walker G.C., Reinhold V.N. Structural studies of a novel exopolysaccharide produced by a mutant of Rhizobium meliloti strain Rm1021. Carbohydr. Res. 198:1990;305-312.
22. Hinterdorfer P., Baumgartner W., Gruber H., Schilcher K., Schindler H. Detection and localization of individual antibody-antigen recognition events by atomic force microscopy. Proc. Natl. Acad. Sci. USA. 93:1996;3477-3481.
23. Hinterdorfer P., Schilcher K., Baumgartner W., Gruber H., Schindler H. A mechanistic study of the dissociation of individual antibody-antigen pairs by atomic force microscopy. Nanobiology. 4:1998;177-188.
24. Hutter J.L., Bechhoefer J. Calibration of atomic-force microscope tips. Rev. Sci. Instrum. 7:1993;1868-1873.
25. Keller M., Roxlau A., Weng W.M., Schmidt M., Quandt J., Niehaus K., Jording D., Arnold W., Pühler A. Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharides succinoglycan and galactoglucan. Mol. Plant Microbe Interact. 8:1995;267-277.
26. Komeda H., Kobayashi M., Shimizu S. Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proc. Natl. Acad. Sci. USA. 93:1996;4267-4272.
27. Lee G.U., Chrisey L.A., Colton R.J. Direct measurement of the forces between complementary strands of DNA. Science. 266:1994;771-773.
28. Lee G.U., Kidwell D.A., Colton R.J. Sensing discrete streptavidin-biotin interactions with atomic force microscopy. Langmuir. 10:1994;354-357.
29. Lloret J., Martin M., Oruezabal R.I., Bonilla I., Rivilla R. MucR and MucS activate exp genes transcription and galactoglucan production in Sinorhizobium meliloti EFB1. Mol. Plant Microbe Interact. 15:2002;54-59.
30. Lloret J., Wulff B.B., Rubio J.M., Downie J.A., Bonilla I., Rivilla R. Exopolysaccharide II production is regulated by salt in the halotolerant strain Rhizobium meliloti EFB1. Appl. Environ. Microbiol. 64:1998;1024-1028.
31. Lyubchenko Y., Shlyakhtenko L., Harrington R., Oden P., Lindsay S. Atomic force microscopy of long DNA: imaging in air and under water. Proc. Natl. Acad. Sci. USA. 90:1993;2137-2140.
32. Merkel R., Nassoy P., Leung A., Ritchie K., Evans E. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy. Nature. 397:1999;50-53.
33. Miller P.F., Sulavik M.C. Overlaps and parallels in the regulation of intrinsic multiple-antibiotic resistance in Escherichia coli. Mol. Microbiol. 21:1996;441-448.
34. Oscarsson J., Mizunoe Y., Uhlin B.E., Haydon D.J. Induction of haemolytic activity in Escherichia coli by the slyA gene product. Mol. Microbiol. 20:1996;191-199.
35. Pellock B.J., Teplitski M., Boinay R.P., Bauer W.D., Walker G.C. A LuxR homolog controls production of symbiotically active extracellular polysaccharide II by Sinorhizobium meliloti. J. Bacteriol. 184:2002;5067-5076.
36. Rawlins D.R., Milman G., Hayward S.D., Hayward G.S. Sequence-specific DNA binding of the Epstein-Barr virus nuclear antigen (EBNA-1) to clustered sites in the plasmid maintenance region. Cell. 42:1985;859-868.
37. Rief M., Gautel M., Oesterhelt F., Fernandez J.M., Gaub H.E. Reversible unfolding of individual titin immunoglobulin domains by AFM. Science. 276:1997;1109-1112.
38. Ros R., Schwesinger F., Anselmetti D., Kubon M., Schäfer R., Plückthun A., Tiefenauer L. Antigen binding forces of individually addressed single-chain Fv antibody molecules. Proc. Natl. Acad. Sci. USA. 95:1998;7402-7405.
39. Rüberg S., Pühler A., Becker A. Biosynthesis of the exopolysaccharide galactoglucan in Sinorhizobium meliloti is subject to a complex control by the phosphate-dependent regulator PhoB and the proteins ExpG and MucR. Microbiology. 145:1999;603-611.
40. Schirmer F., Ehrt S., Hillen W. Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250. J. Bacteriol. 179:1997;1329-1336.
41. Schwesinger F., Ros R., Strunz T., Anselmetti D., Güntherodt H.-J., Honegger A., Jermutus L., Tiefenauer L., Plückthun A. Unbinding forces of singel antibody-antigen complexes correlate with their thermal dissociation rates. Proc. Natl. Acad. Sci. USA. 97:2000;9972-9977.
42. Strunz T., Oroszlan K., Schäfer R., Güntherodt H.-J. Dynamic force spectroscopy of singel DNA molecules. Proc. Natl. Acad. Sci. USA. 96:1999;11277-11282.
43. Sulavik M.C., Gambino L.F., Miller P.F. The MarR repressor of the multiple antibiotic resistance (mar) operon in Escherichia coli: prototypic member of a family of bacterial regulatory proteins involved in sensing phenolic compounds. Mol. Med. 1:1995;436-446.
44. Wang L.X., Wang Y., Pellock B., Walker G.C. Structural characterization of the symbiotically important low-molecular-weight succinoglycan of Sinorhizobium meliloti. J. Bacteriol. 181:1999;6788-6796.
45. Zhan H.J., Lee C.C., Leigh J.A. Induction of the second exopolysaccharide (EPSb) in Rhizobium meliloti SU47 by low phosphate concentrations. J. Bacteriol. 173:1991;7391-7394.
46. Zhan H.J., Levery S.B., Lee C.C., Leigh J.A. A second exopolysaccharide of Rhizobium meliloti strain SU47 that can function in root nodule invasion. Proc. Natl. Acad. Sci. USA. 86:1989;3055-3059.