Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250

Journal of Bacteriology

Volumn 179, Issue 4, 1997, Pages 1329-1336

  Schirmer, Falck ^a   Ehrt, Sabine ^a   Hillen, Wolfgang ^a,b  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHENOL; REGULATOR PROTEIN;

ACINETOBACTER CALCOACETICUS; ARTICLE; BACTERIAL METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN STRUCTURE;

ACINETOBACTER CALCOACETICUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS;

EID: 0031039255     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.4.1329-1336.1997     Document Type: Article

References (50)

1. Abril, M.-A., M. Buck, and J. L. Ramos. 1991. Activation of the Pseudomonas TOL plasmid upper pathway opcron: identification of binding sites for the positive regulator Xy1R and for integration host factor protein. J. Biol. Chem. 266:5832-5838.
2. Abril, M.-A., C. Michan, K. N. Timmis, and J. L. Ramos. 1989. Regulator and enzyme specificities of the TOL plasmid-encoded upper pathway for degradation of aromatic hydrocarbons and expansion of the substrate range of the pathway. J. Bacteriol. 171:6782-6790.
3. Berns, K. I., and C. A. Thomas, Jr. 1965. Isolation of high molecular weight DNA from Haemophilus influenzae. J. Mol. Biol. 11:476-490.
4. Chirgwin, J. M., A. E. Przybyla, R. J. McDonald, and W. J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
5. Cohen-Bazire, G., W. R. Sistrom, and R. Y. Stanier. 1957. Kinetic studies of pigment synthesis by non-sulphur purple bacteria. J. Cell. Comp. Physiol. 49:25-68.
6. Delgado, A., and J. L. Ramos. 1994. Genetic evidence for activation of the positive transcriptional regulator XylR, a member of the NtrC family of regulators, by effector binding. J. Biol. Chem. 269:8059-8062.
7. 54-dependent Pu promoter of TOL plasmid. EMBO J. 10:1159-1167.
8. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 13:385-387.
9. 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus. J. Bacteriol. 176:3493-3499.
10. Ehrt, S., F. Schirmer, and W. Hillen. 1995. Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250. Mol. Microbiol. 18:13-20.
11. Fernàndez, S., V. de Lorenzo, and J. Péres-Martín. 1995. Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains. Mol. Microbiol. 16:205-213.
12. Fernàndez, S., V. Shingler, and V. de Lorenzo. 1994. Cross-regulation by XylR and DmpR activators of Pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes. J. Bacteriol. 176:5052-5058.
13. Fewson, C. A. 1967. The identity of the gram-negative bacterium NCIB8250 ('vibrio 01'). J. Gen. Microbiol. 48:107-110.
14. Fewson, C. A. 1984. Growth yields and respiratory efficiency of Acinetobacter calcoaceticus. J. Gen. Microbiol. 131:865-872.
15. Hanahan, D. 1983. Techniques for transforming E. coli. J. Mol. Biol. 166: 557-580.
16. Hardies, S. C., R. K. Patient, R. D. Klun, F. Ho, W. S. Reznikoff, and R. D. Wells. 1979. Construction and mapping of recombinant plasmids used for preparation of DNA fragments containing the E. coli lactose operator and promoter. J. Biol. Chem. 254:5527-5534.
17. Holmes, D. S., and M. Quigley. 1986. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114:193-197.
18. 54-dependent transcriptional activator FhlA from Escherichia coli. J Bacteriol. 177:2798-2803.
19. Hunger, M., R. Schmucker, V. Kishan, and W. Hillen. 1990. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87:45-51.
20. Inouye, S., A. Nakazawa, and T. Nakazawa, 1988. Nucleotide sequence of the regulatory gene xylR of the TOL plasmid from Pseudomonas putida. Gene 66:301-306.
21. Juni, E. 1978. Genetics and physiology of Acinetobacter. Annu. Rev. Microbiol. 32:349-371.
22. Kokotek, W., and W. Lotz. 1989. Construction of a lacZ-kanamycin-resistance cassette, useful for site-directed mutagenesis and as a promoter probe. Gene 84:467-471.
23. Lehming, N., J. Sartorius, M. Niemöller, G. Genenger, B. von Wilcken-Bergmann, and B. Müller-Hill. 1987. The interaction of the recognition helix of lac repressor with lac operator. EMBO J. 6:3145-3152.
24. Marqués, S., A. Holtel, K. N. Timmis, and J. L. Ramos. 1994. Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWWO of Pseudomonas putida for metabolism of aromatics. J. Bacteriol. 176:2517-2524.
25. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J. Bacteriol. 175:6067-6074.
27. Ng, L. C., C. L. Poh, and V. Shingler. 1995. Aromatic effector activation of the NtrC-like transcriptional regulator PhhR limits the catabolic potential of the (methyl)phenol degradative pathway it controls. J. Bacteriol. 177:1485-1490.
28. Ng, L. C., V. Shingler, C. C. Sze, and C. L. Pok. 1994. Cloning and sequences of the first eight genes of the chromosomally encoded (methyl) phenol degradation pathway from Pseudomonas putida P35X. Gene 151:29-36.
29. Nordlund, I., J. Powlowski, A. Hagstrom, and V. Shingler. 1993. Conservation of regulatory and structural genes for a multi-component phenol hydroxylase within phenol-catabolizing bacteria that utilize a meta-cleavage pathway. J. Gen. Microbiol. 139:2695-2703.
30. Nordlund, I., J. Powlowski, and V. Shingler. 1990. Complete nucleotide and polypeptide analysis of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600. J. Bacteriol. 172:6826-6833.
31. Ornston, L. N., and R. Y. Stanier. 1966. The conservation of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. J. Biol. Chem. 241:3776-3786.
32. Paller, G., R. K. Hammel, and H.-P. Kleber. 1995. Phenol degradation by Acinetobacter calcoaceticus NCIB8250. J. Basic Microbiol. 35:325-335.
33. Patel, R. N., S. Mazumdar, and L. N. Ornston. 1975. β-Ketoadipate enollactone hydrolases I and II from Acinetobacter calcoaceticus. J. Biol. Chem. 250:6567-6577.
34. Pavel, H., M. Forsman, and V. Shingler. 1994. An aromatic effector specificity mutant of the transcriptional regulator DmpR overcomes the growth constraints of Pseudomonas sp. strain CF600 on para-substituted methylphenols. J. Bacteriol. 176:7550-7557.
35. Pérez-Martín, J., and V. de Lorenzo. 1995. The amino-terminal domain of the prokaryotic enhancer-binding protein XylR is a specific intermolecular repressor. Proc. Natl. Acad. Sci. USA 92:9392-9396.
36. 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA. Cell 86:331-339.
37. Pérez-Martín, J., and V. de Lorenzo. 1996. Identification of the repressor subdomain within the signal reception module of the procaryotic enhancer-binding protein XylR of Pseudomonas putida. J. Biol. Chem. 271:7899-7902.
38. 54-dependent transcriptional activator of Pseudomonas putida. J. Mol. Biol. 258:575-587.
39. 54-promoters of the TOL plasmid of Pseudomonas putida. J. Mol. Biol. 258:562-574.
40. Sambrook, J., T. Maniatis, and E. F. Fritsch. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
41. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
42. Schwarz, E., G. Scherrer, G. Hobom, and H. Kössel. 1987. Nucleotide sequence of cro, cII and part of the O gene in phage lambda DNA. Nature (London) 272:410-414.
43. 54-dependent regulators: derepression as a control mechanism. Mol. Microbiol. 19:409-416.
44. Shingler, V., and T. Moore. 1994. Sensing of aromatic compounds by the DmpR transcriptional activator of phenol-catabolizing Pseudomonas sp. strain CF600. J. Bacteriol. 176:1555-1560.
45. Shingler, V., and H. Pavel. 1995. Direct regulation of the ATPase activity of the transcriptional activator DmpR by aromatic compounds. Mol. Microbiol. 17:505-513.
46. Shingler, V., M. Bartilson, and T. Moore. 1993. Cloning and nucleotide sequence of the gene encoding the positive regulator (DmpR) of the phenol catabolitc pathway encoded by pVI150 and identification of DmpR as a member of the NtrC family of transcriptional activators. J. Bacteriol. 175:1596-1604.
47. Stock, J. B., A. M. Stock, and J. M. Mottonen. 1990. Signal transduction in bacteria. Nature (London) 344:395-400.
48. Walker, J. E., M. Saraste, M. J. Runswick, and M. J. Gay. 1982. Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide fold. EMBO J. 1:945-951.
49. Wedel, A., and S. Kustu. 1995. The bacterial enhancer-binding protein NTRC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation. Genes Dev. 9:2042-2052.
50. Williams, J. G., and P. J. Mason. 1985. Hybridization in the analysis of RNA, p. 139-160. In B. D. Hames and S. J. Higgins (ed.), Nucleic acids hybridization - a practical approach. IRL Press, Washington, D.C.