Subcellular localization and N-glycosylation of human ABCC6, expressed in MDCKII cells

Biochemical and Biophysical Research Communications

Volumn 308, Issue 2, 2003, Pages 263-269

  Sinkó, Emese ^a,b   Iliás, Attila ^a   Ujhelly, Olga ^b   Homolya, László ^b   Scheffer, George L ^c   Bergen, Arthur A B ^d   Sarkadi, Balázs ^b   Váradi, András ^a  

^a INSTITUTE OF ENZYMOLOGY   (Hungary)

^b INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^d NETHERLANDS INSTITUTE FOR NEUROSCIENCE   (Netherlands)

Author keywords

ABC transporters; Limited proteolysis; Membrane topology; N glycosylation; Pseudoxanthoma elasticum

Indexed keywords

ABC TRANSPORTER; PROTEIN ANTIBODY;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIBODY DETECTION; ARTICLE; BASOLATERAL MEMBRANE; CONTROLLED STUDY; GENE EXPRESSION; GENE EXPRESSION SYSTEM; GENE LOCATION; GLYCOSYLATION; HUMAN; IMMUNOCYTOCHEMISTRY; IMMUNODETECTION; MAMMAL CELL; NONHUMAN; POLARIZATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; SIGNAL TRANSDUCTION;

MAMMALIA;

EID: 0042093650     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)01349-4     Document Type: Article

References (26)

1. Le Saux O., Urban Z., Tschuch C., Csiszar K., Bacchelli B., Quaglino D., Pasquali-Ronchetti I., Pope F.M., Richards A., Terry S., Bercovitch L., de Paepe A., Boyd C.D. Mutations in a gene encoding an ABC transporter cause pseudoxanthoma elasticum. Nat. Genet. 25:2000;223-227.
2. Bergen A.A., Plomp A.S., Schuurman E.J., Terry S., Breuning M., Dauwerse H., Swart J., Kool M., van Soest S., Baas F., ten Brink J.B., de Jong P.T. Mutations in ABCC6 cause pseudoxanthoma elasticum. Nat. Genet. 25:2000;228-231.
3. Ringpfeil F., Lebwohl M.G., Christiano A.M., Uitto J. Pseudoxanthoma elasticum: mutations in the MRP6 gene encoding a transmembrane ATP-binding cassette (ABC) transporter. Proc. Natl. Acad. Sci. USA. 97:2000;6001-6006.
4. Struk B., Cai L., Zach S., Ji W., Chung J., Lumsden A., Stumm M., Huber M., Schaen L., Kim C.A., Goldsmith L.A., Viljoen D., Figuera L.E., Fuchs W., Munier F., Ramesar R., Hohl D., Richards R., Neldner K.H., Lindpaintner K. Mutations of the gene encoding the transmembrane transporter protein ABC-C6 cause pseudoxanthoma elasticum. J. Mol. Med. 78:2000;282-286.
5. Germain D.P., Remones V., Perdu J., Jeunemaitre X. Identification of two polymorphisms (c189G. > C; c190T > C) in exon 2 of the human MRP6 gene (ABCC6) by screening of Pseudoxanthoma elasticum patients: possible sequence correction? Hum. Mutat. 16:2000;449.
6. Germain D.P., Perdu J., Remones V., Jeunemaitre X. Homozygosity for the R1268Q mutation in MRP6, the pseudoxanthoma elasticum gene, is not disease-causing. Biochem. Biophys. Res. Commun. 274:2000;297-301.
7. Ringpfeil F., Nakano A., Uitto J., Pulkkinen L. Compound heterozygosity for a recurrent 16.5-kb Alu-mediated deletion mutation and single-base-pair substitutions in the ABCC6 gene results in pseudoxanthoma elasticum. Am. J. Hum. Genet. 68:2001;642-652.
8. Le Saux O., Beck K., Sachsinger C., Silvestri C., Treiber C., Goring H.H., Johnson E.W., De Paepe A., Pope F.M., Pasquali-Ronchetti I., Bercovitch L., Marais A.S., Viljoen D.L., Terry S.F., Boyd C.D. A spectrum of ABCC6 mutations is responsible for pseudoxanthoma elasticum. Am. J. Hum. Genet. 69:2001;749-764.
9. Hu X., Plomp A., Wijnholds J., Ten Brink J., Van Soest S., Van Den Born L.I., Leys A., Peek R., De Jong P.T., Bergen A.A. ABCC6/MRP6 mutations: further insight into the molecular pathology of pseudoxanthoma elasticum. Eur. J. Hum. Genet. 11:2003;215-224.
10. Trip M.D., Smulders Y.M., Wegman J.J., Hu X., Boer J.M., ten Brink J.B., Zwinderman A.H., Kastelein J.J., Feskens E.J., Bergen A.A. Frequent mutation in the ABCC6 gene (R1141X) is associated with a strong increase in the prevalence of coronary artery disease. Circulation. 106:2002;773-775.
11. Bakos E., Hegedus T., Hollo Z., Welker E., Tusnady G.E., Zaman G.J., Flens M.J., Varadi A., Sarkadi B. Membrane topology and glycosylation of the human multidrug resistance-associated protein. J. Biol. Chem. 271:1996;12322-12326.
12. Hipfner D.R., Almquist K.C., Leslie E.M., Gerlach J.H., Grant C.E., Deeley R.G., Cole S.P. Membrane topology of the multidrug resistance protein (MRP). A study of glycosylation-site mutants reveals an extracytosolic NH2 terminus. J. Biol. Chem. 272:1997;23623-23630.
13. Kast C., Gros P. Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence. J. Biol. Chem. 272:1997;26479-26487.
14. Kast C., Gros P. Epitope insertion favors a six transmembrane domain model for the carboxy-terminal portion of the multidrug resistance-associated protein. Biochemistry. 37:1998;2305-2313.
15. Ilias A., Urban Z., Seidl T.L., Le Saux O., Sinko E., Boyd C.D., Sarkadi B., Varadi A. Loss of ATP-dependent transport activity in pseudoxanthoma elasticum-associated mutants of human ABCC6 (MRP6). J. Biol. Chem. 277:2002;16860-16867.
16. Cai J., Daoud R., Alqawi O., Georges E., Pelletier J., Gros P. Nucleotide binding and nucleotide hydrolysis properties of the ABC transporter MRP6 (ABCC6). Biochemistry. 41:2002;8058-8067.
17. Belinsky M.G., Chen Z.S., Shchaveleva I., Zeng H., Kruh G.D. Characterization of the drug resistance and transport properties of multidrug resistance protein 6 (MRP6, ABCC6). Cancer Res. 62:2002;6172-6177.
18. Kool M., van der Linden M., de Haas M., Baas F., Borst P. Expression of human MRP6, a homologue of the multidrug resistance protein gene MRP1, in tissues and cancer cells. Cancer Res. 59:1999;175-182.
19. Belinsky M.G., Kruh G.D. MOAT-E (ARA) is a full-length MRP/cMOAT subfamily transporter expressed in kidney and liver. Br. J. Cancer. 80:1999;1342-1349.
20. Scheffer G.L., Hu X., Pijnenborg A.C., Wijnholds J., Bergen A.A., Scheper R.J. MRP6 (ABCC6) detection in normal human tissues and tumors. Lab. Invest. 82:2002;515-518.
21. Madon J., Hagenbuch B., Landmann L., Meier P.J., Stieger B. Transport function and hepatocellular localization of mrp6 in rat liver. Mol. Pharmacol. 57:2000;634-641.
22. Becker S., Wasser S., Hauses M., Hossle J.P., Ott M.G., Dinauer M.C., Ganser A., Hoelzer D., Seger R., Grez M. Correction of respiratory burst activity in X-linked chronic granulomatous cells to therapeutically relevant levels after gene transfer into bone marrow CD34+ cells. Hum. Gene Ther. 9:1998;1561-1570.
23. Ujhelly O., Ozvegy C., Varady G., Cervenak J., Homolya L., Grez M., Scheffer G., Roos D., Bates S.E., Varadi A., Sarkadi B., Nemet K. Application of a human multidrug transporter (ABCG2) variant as selectable marker in gene transfer to progenitor cells. Hum. Gene Ther. 14:2003;403-412.
24. Sarkadi B., Price E.M., Boucher R.C., Germann U.A., Scarborough G.A. Expression of the human multidrug resistance cDNA in insect cells generates a high activity drug-stimulated membrane ATPase. J. Biol. Chem. 267:1992;4854-4858.
25. Klein I., Sarkadi B., Varadi A. An inventory of the human ABC proteins. Biochim. Biophys. Acta. 1461:1999;237-262.
26. Raab-Graham K.F., Cirilo L.J., Boettcher A.A., Radeke C.M., Vandenberg C.A. Membrane topology of the amino-terminal region of the sulfonylurea receptor. J. Biol. Chem. 274:1999;29122-29129.