STR DNA typing: Increased sensitivity and efficient sample consumption using reduced PCR reaction volumes

Journal of Forensic Sciences

Volumn 48, Issue 5, 2003, Pages 1001-1013

  Leclair, Benoît ^a,b   Sgueglia, Joanne B ^c,d   Wojtowicz, Patricia C ^e   Juston, Ann C ^c   Frégeau, Chantal J ^a   Fourney, Ron M ^a  

^a ROYAL CANADIAN MOUNTED POLICE   (Canada)

^b Utah   (United States)

^c Suffolk County Crime Laboratory   (United States)

^d State Police Crime Laboratory   (United States)

^e MN BCA Forensic Science Laboratory   (United States)

Author keywords

Casework; CSF1PO; D13S317; D16S539; D18S51; D21S11; D3S1358; D5S818; D7S820; D8S 1179; DNA typing; FGA; Forensic science; Microfluidics; Polymerase chain reaction; TH01; TPOX; vWA

Indexed keywords

DNA;

ACCURACY; ALLELE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CRIME; DNA FINGERPRINTING; DNA TEMPLATE; FEMALE; FORENSIC SCIENCE; HUMAN; HUMAN EXPERIMENT; MALE; NORMAL HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SAMPLE SIZE; TANDEM REPEAT;

EID: 0041971025     PISSN: 00221198     EISSN: None     Source Type: Journal    
DOI: 10.1520/jfs2002182     Document Type: Article

References (29)

1. Jeffreys AJ, Wilson V, Neumann R, Keyte J. Amplification of human minisatellites by the polymerase chain reaction: towards DNA finger-printing of single cells. Nucleic Acids Res 1988;16(23):10953-71.
2. Edwards A, Civitello A, Hammond HA, Caskey CT. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 1991;49(4):746-56.
3. Reynolds R, Sensabaugh G, Blake E. Analysis of genetic markers in forensic DNA samples using the polymerase chain reaction. Anal Chem 1991;63(1):2-15.
4. Frégeau CJ, Fourney RM. DNA typing with fluorescently tagged short tandem repeats: a sensitive and accurate approach to human identification. BioTechniques 1993;15:100-9.
5. Urquhart A, Kimpton CP, Downes TJ, Gill P. Variation in short tandem repeat sequences - a survey of twelve microsatellite loci for use as forensic identification markers. Int J Legal Med 1994;107:13-20.
6. Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R. Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 1994;55(1):175-89.
7. Lygo JE, Johnson PE, Holdaway DJ, Woodroffe S, Whitaker JP, Clayton TM, et al. The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Legal Med 1994;107(2):77-89.
8. Urquhart A, Oldroyd NJ, Kimpton CP, Gill P. Highly discriminating heptaplex short tandem repeat PCR system for forensic identification. BioTechniques 1995;18(1):116-8, 120-1.
9. Lins AM, Sprecher CJ, Puers C, Schumm JW. Multiplex sets for the amplification of polymorphic short tandem repeat loci-Silver stain and fluorescence detection. BioTechniques 1996;20(5):882-9.
10. Leclair B, Fourney, RM. Automated fluorescent STR technology: simple modifications to ABD's Profiler-II protocol allow for enhanced performance. In: Proceedings of the Eighth International Symposium on Human Identification; 1997 Sept 17-20; Scottsdale (AZ). Madison (WI): Promega Corporation, 1998.
11. Leclair B, Fourney RM. STR DNA typing: Increased sensitivity and efficient sample consumption using reduced PCR reaction volumes for database analysis and casework implications. In: Proceedings of the Ninth International Symposium on Human Identification; 1998 Oct. 7-10; Orlando (FL). Madison (WI): Promega Corporation, 1999.
12. Borys S, Bowen KL, Fourney RM. PCR volume reduction study using bloodstained FTA™ collection cards and capillary electrophoresis. In: Proceedings of the Ninth International Symposium on Human Identification; 1998 Oct. 7-10; Orlando (FL). Madison (WI): Promega Corporation, 1999.
13. AmpFℓSTR® Profiler Plus™ PCR Amplification Kit User's Manual©1997. Perkin Elmer Applied Biosystems, Human Identification Department, San Jose, California.
14. Holt CL, Buoncristiani M, Wallin JM, Nguyen T, Lazaruk KD, Walsh PS. TWGDAM validation of AmpFℓSTR PCR amplification kits for forensic DNA casework. J Forensic Sci 2002;47(1):66-96.
15. Frégeau CJ, Aubin RA, Elliott JC, Gill SS, Fourney RM. Characterization of human lymphoid cell lines GM9947 and GM9948 as intra- and interlaboratory reference standards for DNA typing. Genomics 1995;28: 184-97.
16. Royal Canadian Mounted Police, Forensic Laboratory Services Directorate. Biology Section Methods Guide. Rev. ed. Ottawa, ON, RCMP, 1998.
17. Leclair B, Frégeau CJ, Bowen KL, Fourney RM. AmpFℓSTR© Profiler Plus™ validation: establishing thresholds of stutter percentages and allele ratio at heterozygous loci for use in mixture analysis (in preparation).
18. Frégeau CJ, Bowen KL, Leclair B, Trudel I, Bishop L, Fourney RM. AmpFℓSTR® Profiler Plus™ short tandem repeat DNA analysis of casework samples, mixture samples and non-human DNA samples amplified under reduced PCR volume conditions (25 μL). J Forensic Sci 2003; 48(5):1-13.
19. Clayton TM, Whitaker JP, Sparkes R, Gill P. Analysis and interpretation of mixed forensic stains using DNA STR profiling. Forensic Sci Int 1998;91(1):55-70.
20. Leclair B, Frégeau CJ, Bowen KL, Fourney RM. Profiler-Plus Validation: Establishing thresholds of stutter percentages and allele ratio at heterozygous loci for use in mixture analysis. In: Proceedings of the Ninth International Symposium on Human Identification; 1998 Oct. 7-10; Orlando (FL). Madison (WI): Promega Corporation, 1999.
21. Gill P, Sparkes R, Pinchin R, Clayton T, Whitaker J, Buckleton J. Interpreting simple STR mixtures using allele peak areas. Forensic Sci Int 1998;91(1):41-53.
22. Evett IW, Gill PD, Lambert JA. Taking account of peak areas when interpreting mixed DNA profiles. J Forensic Sci 1998;43(1):62-9.
23. Perlin MW, Szabady B. Linear mixture analysis: a mathematical approach to resolving mixed DNA samples. J Forensic Sci 2001;46(6): 1372-8.
24. Gill P, Whitaker J, Flaxman C, Brown N, Buckleton J. An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci Int 2000;112(1):17-40.
25. Whitaker JP, Cotton EA, Gill P. A comparison of the characteristics of profiles produced with the AMPFℓSTR SGM Plus multiplex system for both standard and low copy number (LCN) STR DNA analysis. Forensic Sci Int 2001;123(2-3):215-23.
26. Baltimore D. Our genome unveiled. Nature 2001;409:814-6.
27. Robertson JM, Sgueglia JB, Badger CA, Juston AC, Ballantyne J. Forensic applications of a rapid, sensitive, and precise multiplex analysis of the four short tandem repeat loci HUMVWF31/A, HUMTHO1, HUMF13A1, and HUMFES/FPS. Electrophoresis 1995;16:1568-76.
28. Andersen JF, Greenhalgh MJ, Butler HR, Kilpatrick SR, Piercy RC, Way KA, et al. Further validation of a multiplex STR system for use in routine forensic identity testing. Forensic Sci Int 1996;78:47-64.
29. Wallin JM, Holt CL, Lazaruk KD, Nguyen TH, Walsh PS. Constructing universal multiplex PCR systems for comparative genotyping. J Forensic Sci 2002;47(1):52-65.