Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: In vivo measurements using the fluorescent dyes SBFI and PBFI

Journal of Experimental Botany

Volumn 54, Issue 390, 2003, Pages 2035-2043

  Halperin, Stephen J ^a   Lynch, Jonathan P ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

Arabidopsis; Cytosolic K; Cytosolic Na; Fluorescent dyes; Root hairs; Salinity

Indexed keywords

DYES; FLUORESCENCE; PLANTS (BOTANY); SODIUM; TOXICITY;

SALINIZATION;

SALINITY MEASUREMENT;

FLUORESCENT DYE; POTASSIUM; SODIUM; SODIUM CHLORIDE;

ARABIDOPSIS; ARTICLE; CYTOSOL; DRUG EFFECT; FLUORESCENCE MICROSCOPY; KINETICS; METABOLISM; OSMOLARITY; PLANT ROOT; TRANSPORT AT THE CELLULAR LEVEL;

ARABIDOPSIS; BIOLOGICAL TRANSPORT; CYTOSOL; FLUORESCENT DYES; KINETICS; MICROSCOPY, FLUORESCENCE; OSMOLAR CONCENTRATION; PLANT ROOTS; POTASSIUM; SODIUM; SODIUM CHLORIDE;

ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0041875086     PISSN: 00220957     EISSN: None     Source Type: Journal    
DOI: 10.1093/jxb/erg219     Document Type: Article

References (42)

1. Amtmann A, Fischer M, Marsh EL, Stefanovic A, Sanders D, Schachtman DP. 2001. The wheat cDNA LCT1 generates hypersensitivity to sodium in a salt-sensitive yeast strain. Plant Physiology 126, 1061-1071.
2. + antiport in Arabidopsis. Science 285, 1256-1258.
3. Bates T, Lynch J. 2000. Plant growth and phosphorus accumulation of wild type and two root hair mutants of Arabidopsis thaliana (Brassicaceae). American Journal of Botany 87, 958-963.
4. Brauer D, Otto J, Tu SI. 1995. Selective accumulation of the fluorescent pH indicator, BCECF, in vacuoles of maize root-hair cells. Journal of Plant Physiology 145, 57-61.
5. +-selective microelectrode for intracellular measurements in plant cells. Journal of Experimental Botany 52, 1353-1359.
6. + to cell salt tolerance. Plant Physiology 131, 676-683.
7. 2+. Plant Physiology 83, 510-516.
8. Cuin TA, Miller AJ, Laurie SA, Leigh RA. 2003. Potassium activities in cell compartments of salt-grown barley leaves. Journal of Experimental Botany 54, 657-661.
9. Cuin TA, Miller AJ, Laurie SA, Leigh RA. 1999. Nitrate interference with potassium-selective microelectrodes. Journal of Experimental Botany 50, 1709-1712.
10. Davenport RJ, Tester M. 2000. A weakly voltage-dependent, non-selective cation channel mediates toxic sodium influx in wheat. Plant Physiology 122, 823-834.
11. Demidchik V, Tester M. 2002. Sodium fluxes through nonselective cation channels in the plasma membrane of protoplasts from Arabidopsis roots. Plant Physiology 128, 379-387.
12. + efflux in Arabidopsis thaliana salt tolerance. Plant, Cell and Environment 24, 733-740.
13. 2+-selective microelectrode tests and Fura-dextran ratio imaging. Plant Physiology 114, 39-45.
14. Foreman J, Dolan L. 2001. Root hairs as a model system for studying plant cell growth. Annals of Botany 88, 1-7.
15. Gruwel MLH, Rauw VL, Loewen M, Abrams SR. 2001. Effects of sodium chloride on plant cells; a P-31 and Na-23 NMR system to study salt tolerance. Plant Science 160, 785-794.
16. 2+ indicators with greatly improved fluorescence properties. Journal of Biological Chemistry 260, 3440-3450.
17. -, and osmotic adjustment in Lycopersicon pimpinellifolium and L. esculentum during short- and long-term exposures to NaCl. Physiologia Plantarum 97, 583-591.
18. Halperin SH, Kochian LV, Lynch JP. 1997. Salinity stress inhibits calcium loading into the xylem of excised barley (Hordeum vulgare L.) roots. New Phytologist 135, 419-427.
19. Halperin SH, Gilroy S, Lynch JP. 2003. Sodium chloride reduces growth and cytosolic calcium, but does not affect cytosolic pH, in root hairs of Arabidopsis thaliana L. Journal of Experimental Botany 54, 1269-1280.
20. + in individual fibroblasts and lymphocytes. Journal of Biological Chemistry 264, 19458-19467.
21. 2+ fluxes in maize roots and maize suspension cells. Planta 188, 601-610.
22. +-binding fluorescent dye benzofuran isophthalate. Planta 195, 525-529.
23. Lindberg S, Strid H. 1997. Aluminum induces rapid changes in cytosolic pH and free calcium and potassium concentrations in root protoplasts of wheat (Triticum aestivum). Physiologia Plantarum 99, 405-414.
24. Lynch JP, Thiel G, Läuchli A. 1988. Effects of salinity on the extensibility and Ca availability in the expanding region of growing barley leaves. Botanica Acta 101, 355-361.
25. Maathuis FJM, Sanders D. 2001. Sodium uptake in Arabidopsis roots is regulated by cyclic nucleotides. Plant Physiology 127, 1617-1625.
26. +-channel types in root-cells of Arabidopsis thaliana. Planta 197, 456-464.
27. Memon AR, Saccomani M, Glass ADM. 1985. Efficiency of potassium utilization by barley varieties: the role of subcellular compartmentation. Journal of Experimental Botany 36, 1860-1876.
28. Minta A, Tsien RY. 1989. Fluorescent indicators for sodium. Journal of Biological Chemistry 264, 19449-19457.
29. + in intact leaves of cotton by in vivo fluorescence ratio-imaging. Functional Plant Biology 29, 1491-1499.
30. + in intact leaves by ratio imaging of PBFI fluorescence. Journal of Experimental Botany 48, 1609-1614.
31. + in intact mung bean roots under high NaCl stress. Plant Cell Physiology 31, 815-821.
32. Olt S, Krotz E, Komor E, Rokitta M, Haasa A. 2000. Na-23 and H-1 microimaging of intact plants. Journal of Magnetic Resonance 144, 297-304.
33. Peterson RL, Farquhar ML. 1996. Root hairs: specialized tubular cells extending root surfaces. The Botanical Review 62, 1-40.
34. + exchanger in Arabidopsis thaliana, by sos2 and sos3. Proceedings of the National Academy of Sciences, USA 99, 8436-8441.
35. + homeostasis. Proceedings of the National Academy of Sciences, USA 99, 9061-9066.
36. + transporters in corn roots: effect on fluxes and their oscillations in the elongating region. Journal of Membrane Biology 161, 45-54.
37. Silberbush M, Ben-Asher J. 1987. The effect of salinity on parameters of potassium and nitrate uptake of cotton. Communications in Soil Science and Plant Analysis 8, 65-81.
38. Tarakcioglu C, Inal A. 2002. Changes induced by salinity, demarcating specific ion ratio (Na/Cl) and osmolality in ion and proline accumulation, nitrate reductase activity, and growth performance of lettuce. Journal of Plant Nutrition 25, 27-41.
39. Trivedi S, Galiba G, Sankhla N, Erdei L. 1991. Responses to osmotic and NaCl stress of wheat varieties differing in drought and salt tolerance in callus cultures. Plant Science 73, 227-232.
40. Tsien RY, Poenie M. 1986. Fluorescence ratio imaging: a new window into intracellular ionic signaling. Trends in Biochemical Sciences 11, 450-455.
41. Tyerman SD, Skerrett IM. 1999. Root ion channels and salinity. Scientia Horticulturae 78, 175-235.
42. Walker DJ, Leigh RA, Miller AJ. 1996. Potassium homeostasis in vacuolated plant cells. Proceedings of the National Academy of Sciences, USA 93, 10510-10514.