Double strand DNA cleavage by a dinuclear Cu(II) complex

Chemistry Letters

Volumn 32, Issue 6, 2003, Pages 490-491

  Kim, Jung Hee ^a   Kim, Soo Hyun ^a  

^a SunMoon University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

1,3 BIS(1,4,7 TRIAZA 1 CYCLONONYL)PROPANE; COPPER COMPLEX; DOUBLE STRANDED DNA; METAL ION; OXYGEN; PLASMID DNA; PROPANE; UNCLASSIFIED DRUG;

ARTICLE; DEGRADATION; DNA BINDING; DNA CLEAVAGE; HYDROLYSIS; PH; TEMPERATURE;

EID: 0041760725     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.2003.490     Document Type: Article

References (24)

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8. 3) were performed by the same method as described above. The same experiments on DNA cleavage by the CuL2 complex could not be done since the reactions were not fast enough to use enzymes. Product analysis and Quantitation: cleavage products were analyzed in 1% agarose gels. The gels were stained in buffer containing 1 μg/mL ethidium bromide and the extent of DNA degradation was determined by using volume quantitation method with Bio-Rad Molecular Analyst/ PC software version 1.4. The relative amounts of the different forms of DNA were determined by dividing absorbance intensity for a particular band by the total intensities of the each band in the same lane. The correction factor of 1.22 for form I DNA obtained from the calibration experiments was utilized.
9. DNA cleavage was performed at pH 6.7 (MES), 7.3 and 8.0 (HEPES), 8.9 and 9.5 (CHES).
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11. 2L1 complex was considered.
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