Cryo-fracturing and cryo-planing for in-lens cryo-SEM, using a newly designed diamond knife

Microscopy and Microanalysis

Volumn 9, Issue 4, 2003, Pages 279-285

  Walther, Paul ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Cryo; Cryo planing; Cryo sectioning; Endoplasmic reticulum; Freeze etching; Freeze fracturing; High pressure freezing; In lens SEM

Indexed keywords

ANIMAL; CONFERENCE PAPER; ENDOPLASMIC RETICULUM; FREEZE FRACTURE; INSTRUMENTATION; METHODOLOGY; PANCREAS; RAT; SCANNING ELECTRON MICROSCOPY; ULTRAMICROTOMY; ULTRASTRUCTURE;

ANIMALS; CRYOULTRAMICROTOMY; ENDOPLASMIC RETICULUM; FREEZE FRACTURING; MICROSCOPY, ELECTRON, SCANNING; PANCREAS; RATS;

ANIMALIA;

EID: 0041738319     PISSN: 14319276     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1431927603030496     Document Type: Conference Paper

References (21)

1. BASTACKY, J., LEE, C., FREEMAN, T., WEBER, G., BAEZA, A., HUBBINS, T. & CHEN, Y. (1995). A specimen holder for high-resolution low-temperature scanning electron microscopy. Microsc Res Tech 32, 457-458.
2. BRANTON, D. (1966). Fracture faces of frozen membranes. Proc Natl Acad Sci, USA 55, 1048-1056.
3. ECHLIN, P. (1992). Low-Temperature Microscopy and Analysis. New York and London: Plenum Press.
4. HERMANN, R. & MÜLLER, M. (1993). Progress in scanning electron microscopy of frozen-hydrated biological specimens. Scanning Microsc 7, 343-350.
5. MICHEL, M., HILLMANN, T. & MÜLLER, M. (1991). Cryo-sectioning of plant material frozen at high pressure. J Microsc 163, 3-18.
6. MOOR, H. & BELLIN, G. (1980). The influence of high pressure freezing on mammalian nerve tissue. Cell Tissue Res 209, 201-216.
7. MOOR, H. & MÜHLETHALER, K. (1963). Fine structure in frozen-etched yeast cells. J Cell Biol 17, 609-628.
8. MOOR, H. & RIEHLE, U. (1968). Snap-freezing under high pressure: A new fixation technique for freeze-etching. Proc 4th European Reg. Conf. of Electron Microsc 2, 33-34.
9. MÜLLER, M. & MOOR, H. (1984). Cryo-fixation of suspensions and tissues by propane jet freezing and high-pressure freezing. Proceedings of the 42nd Annual Meeting of the Electron Microscopy Society of America, Bailey, G.W. (Ed.), pp. 6-9. San Francisco: San Francisco Press.
10. NIJSSE, J. & VAN AELST, A.C. (1999). Cryo-planing for cryo-scanning electron microscopy. Scanning 21, 372-378.
11. ORCI, L., PERRELET, A. & LIKE, A.A. (1972). Fenestrae in the rough endoplasmic reticulum of the exocrine pancreatic cells. J Cell Biol 55, 245-249.
12. SHIMONI, E. & MÜLLER, M. (1998). On optimizing high-pressure freezing: From heat transfer theory to a new microbiopsy device. J Microsc 192, 236-247.
13. STEERE, R.L. (1957). Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol 3, 45-60.
14. STUDER, D., GRABER, W., AL-AMOUDI, A. & EGGLI, P. (2001). A new approach for cryofixation by high-pressure freezing. J Microsc 203, 285-294.
15. STUDER, D., MICHEL, M. & MULLER, M. (1989). High pressure freezing comes of age. Scanning Microsc 3 (Suppl. 3), 253-268.
16. STUDER, D., MICHEL, M., WOHLWEND, M., HUNZIKER, E.B. & BUSCHMANN, M.D. (1995). Vitrification of articular cartilage by high-pressure freezing. J Microsc 179, 321-332.
17. WALTHER, P., CHEN, Y., PECH, L.L. & PAWLEY, J.B. (1992). High resolution scanning electron microscopy of frozen-hydrated cells. J Microsc 168, 169-180.
18. WALTHER, P. & HENTSCHEL, J. (1989). Improved representation of cell surface structures by freeze substitution and backscattered electron imaging. Scanning Microsc 3 (Suppl. 3), 201-211.
19. WALTHER, P. & MÜLLER, M. (1999). Biological ultrastructure as revealed by high resolution cryo-SEM of blockfaces after cryo-sectioning. J Microsc 196, 279-287.
20. WALTHER, P., WEHRLI, E., HERMANN, R. & MÜLLER, M. (1995). Double layer coating for high resolution low temperature SEM. J Microsc 179, 229-237.
21. WALTHER, P. & ZIEGLER, A. (2002). Freeze substitution of high-pressure frozen samples: The visibility of biological membranes is improved when the substitution medium contains water. J Microsc 208, 3-10.