The influence of chromatin structure on initial DNA damage and radiosensitivity in CHO-K1 and xrs1 cells at low doses of irradiation (1-10 Gy)

Radiation and Environmental Biophysics

Volumn 41, Issue 3, 2002, Pages 199-206

  Roos, W P ^a   Binder, A ^a   Bohm L ^a  

^a UNIVERSITY OF STELLENBOSCH   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

COBALT 60; DNA;

ANIMAL CELL; ARTICLE; CELL SURVIVAL; CHO CELL; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA DAMAGE; FLOW CYTOMETRY; HAMSTER; IRRADIATION; NONHUMAN; RADIATION DOSE; RADIOSENSITIVITY;

ANIMALS; CELL DIVISION; CELL LINE; CELL SURVIVAL; CHO CELLS; CHROMATIN; COBALT ISOTOPES; CRICETINAE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, RADIATION; FLOW CYTOMETRY; NOCODAZOLE; RADIATION DOSAGE; RADIATION TOLERANCE; REFERENCE VALUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SODIUM CHLORIDE;

ANIMALIA; CRICETINAE; TRIXIS;

EID: 0041730509     PISSN: 0301634X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00411-002-0166-3     Document Type: Article

References (39)

1. Schwartz JL, Mustafi R, Beckett MA, Weichselbaum RR (1990) Prediction of the sensitivity of human squamous cell carcinoma cells using DNA filter elution. Radiat Res 123:1-6
2. Ruiz De Almodovar JM, Nunez MI, McMillan TJ, Olea N, Mort C, Villalobos M, Pedraza V, Steel GG (1994) Initial radiation-induced DNA damage in human tumour cell lines: a correlation with intrinsic cellular radiosensitivity. Br J Cancer 69:457-462
3. Whitaker SJ, Ung YC, McMillan TJ (1995) DNA double-strand break induction and rejoining as determinants of human tumour cell radiosensitivity. A pulsed-field gel electrophoresis study. Int J Radiat Biol 67:7-18
4. Dikomey E, Dahm-Daphi J, Bremmer I, Martensen R, Kaina B, (1998) Correlation between cellular radiosensitivity and non-repaired double-strand breaks studied in nine mammalian cell lines. Int J Radiat Biol 73:269-278
5. Frankenberg-Schwager M (1989) Review of repair kinetics for DNA damage induced in eukaryotic cells in vitro by ionising radiation. Radiother Oncol 14:307-320
6. Roos WP, Binder A, Bohm L (2000) Determination of the initial DNA damage and residual DNA damage remaining after 12 hours of repair in eleven cell lines at low doses of irradiation. Int J Radiat Biol 76:1493-1500
7. Radford IR (1986) Evidence for a general relationship between the induced level of DNA double strand breakage and cell killing after X-irradiation of mammalian cells. Int J Radiat Biol 49:611-620
8. Radford IR (1983) Effects of hyperthermia on the repair of X-ray induced DNA double strand breaks in mouse cells. Int J Radiat Biol 432:551-557
9. Prise KM, Davies S, Michael BD (1987) The relationship between radiation induced DNA double strand breaks and cell kill in hamster V79-fibroblasts irradiated with 250 kVp X-rays, 2.3 MeV neutrons or 238Pu alpha-particles. Int J Radiat Biol 52:893-902
10. Goodhead DT (1989) The initial physical damage produced by ionizing radiations. Int J Radiat Biol 56:623-634
11. Charlton DE, Nikjoo H, Humm JL (1989) Calculation of initial yields of single- and double-strand breaks in cell nuclei from electrons, protons and alpha particles. Int J Radiat Biol 56:1-19
12. Nikjoo H, Uehara S, Wilson WE, Hosh M, Goodhead DT (1998) Track structure in radiation biology: theory and applications. Int J Radiat Biol 73:355-364
13. Friedland W, Jacob P, Paretzke HG, Stork T (1998) Monte Carlo simulation of the production of short DNA fragments by low-linear energy transfer radiation using higher-order DNA models. Radiat Res 150:170-182
14. Nackerdien Z, Michie J, Bohm L (1989) Chromatin decondensed by acetylation shows an elevated radiation response. Radiat Res 117:234-244
15. Heussen C, Nackerdien Z, Smit BJ, Bohm L (1987) Irradiation damage in chromatin isolated from V-79 Chinese hamster lung fibroblasts. Radiat Res 110:84-94
16. Lyungman M (1991) The influence of chromatin structure on the frequency of radiation-induced DNA strand breaks: a study using nuclear and nucleoid monolayers. Radiat Res 126:58-64
17. Terasima R, Tolmach LJ (1963) X-ray sensitivity and DNA synthesis in synchronous populations of HeLa cells. Science 140:490-492
18. Biade S, Stobbe CC, Chapman JD (1997) The intrinsic radiosensitivity of some human tumor cells throughout their cell cycles. Radiat Res 147:416-421
19. Iliakis GE, Okayasu R (1990) Radiosensitivity throughout the cell cycle and repair of potentially lethal damage and DNA double-strand breaks in an X-ray-sensitive CHO mutant. Int J Radiat Biol 57:1195-1211
20. Iliakis G, Kurtzman S, Pantelias G, Okayasu R (1989) Mechanism of radiosensitization by halogenated pyrimidines: effect of BrdU on radiation induction of DNA and chromosome damage and its correlation with cell killing. Radiat Res 119:286-304
21. Brabander RA, Van de Veire RML, Aerts FEM, Borgers M, Janssen PAJ (1976) The effects of methyl [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl] carbamate (R 17934; NSC 238159), a new synthetic antitumoral drug interfering with microtubules, on mammalian cells cultured in vitro. Cancer Res 36:905-916
22. Szekely JG, Raaphorst GP, Copps TP, Lobreau AU, Azzam EA, (1983) Effect of anisotonic NaCl treatment on cellular ultrastructure of V79 Chinese hamster cells. Part 1. Mitotic cells. Int J Radiat Biol Relat Stud Phys Chem Med 43:115-122
23. Iliakis GE, Pantelias GE (1990) Production and repair of chromosome damage in an X-ray sensitive CHO mutant visualized and analysed in interphase using the technique of premature chromosome condensation. Int J Radiat Biol 57:1213-1223
24. Singleton K, Priestley A, Steingrimsdottir H, Gell D, Blunt T, Jackson SP, Lehmann AR, Jeggo PA (1997) Molecular and biochemical characterisation of xrs mutants defective in Ku80. Mol Cell Biol 17:1264-1273
25. Juan G, Traganos F, James WM, Ray JM, Roberge M, Sauve DM, Anderson H, Darzynkiewicz Z (1998) Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis. Cytometry 32:71-77
26. Chapman JD, Stobbe CC, Matsumoto Y (2001) Chromatin compaction and tumor cell radiosensitivity at 2 gray. Am J Clin Oncol 24:509-515
27. Wilson P (1992) Cytotoxicity and viability assays. In: Freshney RI (ed) Animal cell culture: a practical approach, 2nd edn. IRL Press at Oxford University Press, Oxford, pp 263-303
28. Kiltie E, Orton CJ, Ryan AJ, Roberts SA, Marples B, Davidson SE, Hunter RD, Margison GP, West CM, Hendry JH (1997) A correlation between residual DNA double-strand breaks and clonogenic measurements of radiosensitivity in fibroblasts from preradiotherapy cervix cancer patients. Int J Radiat Oncol Biol Phys 39:1137-1144
29. Birnboim HC, Jevcak JJ (1981) Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation. Cancer Res 41:1889-1892
30. Dahm-Daphi J, Dikomey E, Pyttlik C (1994) Relationship between non-reparable DNA strand breaks and cell survival studied in X-irradiated CHO, CHO-K1, xrs1 and xrs5 cells. Int J Radiat Biol 65:657-663
31. Ning S, Knox SJ (1999) G2/M-phase arrest and death by apoptosis of HL60 cells irradiated with exponentially decreasing low-dose-rate gamma radiation. Radiat Res 151:659-669
32. Chapman JD, Stobbe CC, Gales T, Das IJ, Zellmer DL, Biade S, Matsumoto Y (1999) Condensed chromatin and cell inactivation by single-hit kinetics. Radiat Res 151:433-441
33. Iliakis G, Okayasu R, Varlotto J, Shernoff C, Wang Y (1993) Hypertonic treatment during premature chromosome condensation allows visualization of interphase chromosome breaks repaired with fast kinetics in irradiated CHO cells. Radiat Res 135:160-170
34. Nygren J, Ljungman M, Ahnstrom G (1995) Chromatin structure and radiation induced DNA strand breaks in human cells: soluble scavengers and DNA-bound proteins offer a better protection against single- and double-strand breaks. Int J Radiat Biol 68:11-18
35. Radford IR, Broadhurst S (1986) Enhanced induction by X-irradiation of DNA-double strand breaks in mitotic as compared to S-phase V79 cells. Int J Radiat Biol 9:909-914
36. Giulotto E, Mottura A, De Carli L, Nuzzo F (1978) DNA repair in UV-irradiated heteroploid cells at different phases of the cell cycle. Exp Cell Res 113:415-420
37. Johnston PJ, Bryant PE (1994) A component of double-strand break repair is dependent on the spatial orientation of the lesions within the higher-order structures of chromatin. Int J Radiat Biol 66:531-536
38. Rydberg B (1984) Repair of double-strand breaks in colcemid-arrested mitotic Chinese hamster cells. Int J Radiat Biol 46:299-304
39. Schwartz JL, Cowen JM, Moan E, Sedita BA, Stephens J, Vaughan AT (1993) Metaphase chromosome and nucleoid differences between CHO-K1 and its radiosensitive derivative xrs-5. Mutagenesis 8:105-108