Histone H3 phosphorylation and expression of cyclins A and B1 measured in individual cells during their progression through G2 and mitosis

Cytometry

Volumn 32, Issue 2, 1998, Pages 71-77

  Juan, Gloria ^a   Traganos, Frank ^a   James, William M ^b   Ray, Jill M ^b   Roberge, Michel ^c   Sauve, Debra M ^c   Anderson, Hilary ^c   Darzynkiewicz, Zbigniew ^a  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b Oncor Inc   (United States)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Cell cycle; Chromatin condensation; Prophase; Staurosporine

Indexed keywords

ALKALINE PHOSPHATASE; CYCLIN A; CYCLIN B; DIMETHYL SULFOXIDE; DNA; HISTONE H3; MONOCLONAL ANTIBODY; STAUROSPORINE; VINBLASTINE;

ARTICLE; CELL CYCLE G2 PHASE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEUKEMIA CELL; MITOSIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION;

ALKALINE PHOSPHATASE; ANTIBODIES, MONOCLONAL; CELL CYCLE; CHROMATIN; CYCLIN A; CYCLIN B; DNA, NEOPLASM; G2 PHASE; HISTONES; HL-60 CELLS; HUMANS; INTERPHASE; KINETICS; LEUKEMIA; MITOSIS; MULTIVARIATE ANALYSIS; PHOSPHORYLATION; PHOSPHOSERINE; STAUROSPORINE; TUMOR CELLS, CULTURED; VINBLASTINE;

TRIXIS;

EID: 0032102860     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980601)32:2<71::AID-CYTO1>3.0.CO;2-H     Document Type: Article

References (38)

1. Ajiro K, Nishimoto T: Specific site of histone H3 phosphorylation related to the maintenance of premature chromosome condensation. Evidence for catalytically induced interchange of the subunits. J Biol Chem 260:15379-15381, 1985.
2. Ajiro K, Nishimoto T, Takahashi T: Histone H1 and H3 phosphorylation during premature chromosome condensation in a temperature-sensitive mutant (tsBN2) of baby hamster kidney cells. J Biol Chem 258:4534-4538, 1983.
3. 1 cyclins in the next cycle. Cell 77:1037-1050, 1994.
4. Balhorn R, Jackson V, Granner D, Chalkley R: Phosphorylation of the lysine-rich histones throughout the cell cycle. Biochemistry 14:2504-2511,1975.
5. Bruno S, Crissman, HA, Bauer KD, Darzynkiewicz Z: Changes in cell nuclei during S phase: Progressive chromatin condensation and altered expression of the proliferation-associated nuclear proteins Ki-67, cyclin (PCNA), p105 and p34. Exp Cell Res 196:99-106, 1991.
6. Clevenger CV, Epstein AL, Bauer KD: Quantitative analysis of a nuclear antigen in interphase and mitotic cells. Cytometry 8:280-286, 1987.
7. Darzynkiewicz Z, Gong J, Juan G, Ardelt B, Traganos F: Cytometry of cyclin proteins. Cytometry 23:1-13, 1996.
8. Darzynkiewiez Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F: Cytometry in cell necrohiology: Analysis of apoptosis and accidental cell death. Cvtometry 27:1-20, 1997.
9. Darzynkiewicz Z, Traganos F, Kimmel M: Assay of cell cycle kinetics by multivariate flow cytometry using the principle of stathmokinesis. In: Techniques in Cell Cycle Analysis, Gray JW, Darzynkiewicz Z (eds). Humana Press, Clifton, NJ, 1986, pp 291-331.
10. Darzynkiewicz Z, Traganos F, Melamed MR: New cell compartments identified by multiparameter flow cytometry. Cytometry 1:98-108, 1980.
11. Darzynkiewicz Z, Traganos F, Sharpless T, Melamed MR: Interphase and metaphase chromatin: Different stainability of DNA with acridine orange after treatment at low pH. Exp Cell Res 110:201-214, 1977.
12. Di Vinci A, Geido E, Pfeffer U, Vidali G, Giaretti W: Quantitative analysis of mitotic and early-G1 cells using monoclonal antibodies against AF-2 protein, Cytometry 14:421-427, 1993.
13. Gong J, Traganos F, Darzynkiewicz Z: Staurosporine blocks cell progression through G1 between the cyclin D and cyclin E restriction points. Cancer Res 54:3136-3139, 1994.
14. Gong J, Traganos F, Darzynkiewicz Z: Growth imbalance and altered expression of cyclins B1, A E and D 3 in MOLT-4 cells synchronized in the cell cycle by inhibitors of DNA replication. Cell Growth Differ 6:1485-1493, 1995.
15. 2 and mitotic cells by flow cytometry based on different expression of cyclins A and B1. Exp Cell Res 220:226-231, 1995.
16. Gorczyca W, Melamed MR, Darzynkiewicz Z: Laser scanning cytometer (LSC) analysis of fraction of labeled mitoses. Cell Prolif 29:539-547,1996.
17. cdc2 activity and histone H1 hypcrphosphorylation, but is associated with enhanced histone H2A and H3 phosphorylation EMBO J 14:976-985, 1995.
18. Gurley LR, Walters RA, Barham SS, Daeven LL: Heterochromatin and histone phosphorylation. Exp Cell Res 11:373-383, 1978.
19. Gurley LR, Walters RA, Tobey RA: Sequential phosphorylation of histone subtractions in the Chinese hamster cells. J Biol Chem 250:3936-3944, 1975.
20. Hanks SK, Rodriguez LV, Rao PN: Relationship between histone phosphorylation and premature chromatin condensation. Exp Cell Res 148:293-302, 1983.
21. Juan G, Li X, Darzynkiewicz Z: Correlation between DNA replication and expression of cyclins A and B1 in individual MOLT-4 cells. Cancer Res 57:803-807, 1997.
22. Juan G, Pan W, Darzynkiewicz Z: DNA segments sensitive to single strand specific nucleases are present in chromatin of mitotic cells. Exp Cell Res 227:197-202, 1996.
23. Landberg G, Tan EM, Ross G: Flow cytometric multiparameter analysis of proliferating cell nuclear antigen/cyclin and Ki-67 antigen: A new view of the cell cycle. Exp Cell Res 187:111-118, 1990.
24. Larsen JK, Munch-Petersen B, Christiansen J, Jorgensen K: Flow cytometric discrimination of mitotic cells: Resolution of M, as well as G1, S and G2 phase nuclei with mithramycin, propidium iodide, and ethidium bromide after fixation with formaldehyde. Cytometry 7:54-63, 1986.
25. Li X, Melamed MR, Darzynkiewicz Z: Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. Exp Cell Res 222:28-37, 1996.
26. Luther E, Kamentsky LA: Resolution of mitotic cells using laser scanning cytometry. Cytometry 23:272-278, 1996.
27. Nigg EA: Cyclin-dependent protein kinases: Key regulators of the eukaryotic cell cycle. Bioassays 17:471-480, 1995.
28. Nusse M, Beisker W, Hoffman C, Tarnok A: Flow cytometric analysis of G1- and G2/M phase subpopulations in mammalian cells nuclei using side scatter and DNA content measurements. Cytometry 11:813-821,1990.
29. Paulson JR, Taylor SS: Phosphorylation of histone 1 and 3 and nonhistone high mobility group 14 by an endogenous kinase in HeLa metaphase chromosomes. J Biol Chem 257:6064-6072, 1982.
30. Pines J: Cyclins and cyclin-dependent kinases: Theme and variations. Adv Cane Res 66:181 -212, 1995.
31. cdc2 Cell 58:833-846, 1989.
32. Pines J, Hunter T: Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport. J Cell Biol 115:1-17, 1991.
33. Roti Roti JL, Higashikubo R, Blair OC, Uygur N: Cell-cycle position and nuclear protein content. Cytometry 3:91-96, 1982.
34. Shibata K, Ajiro K: Cell cycle-dependent suppressive effect of histone HI on mitosis-specific H3 phosphorylation. J Biol Chem 268:18431-18434, 1993.
35. Th'ng JPH, Guo X-W, Swank RA, Crissman HA, Bradbury ME: Inhibition of histone phosphorylation by Staurosporine leads to chromosome decontlensation. J Biol Chem 269:9568-9573, 1994.
36. van Holde KE: Chromatin. Springer-Verlag, New York, 1978.
37. Wolffe A: Chromatin. Structure and Function. Academic Press, San Diego, 1992.
38. Zucker RM, Elstein KH, Easterling RA, Massaro EJ: Flow cytometric discrimination of mitotic nuclei by right-angle scatter. Cytometry 9:226-231,1988.