Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype

Plant Molecular Biology

Volumn 52, Issue 4, 2003, Pages 865-872

  Lin, Ming ^a   Behal, Robert ^a   Oliver, David J ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Arabidopsis mutations; Dihydrolipoyl acetyltransferase; E2 subunit; Embryogenesis; Lipid synthesis; Pyruvate dehydrogenase complex; T DNA knockout

Indexed keywords

DNA; ENZYMES; GENES;

MULTI-ENZYME COMPLEXES;

PLANTS (BOTANY);

ARABIDOPSIS; DNA, BACTERIAL; GENES, LETHAL; GENETIC COMPLEMENTATION TEST; MUTAGENESIS, INSERTIONAL; MUTATION; PHENOTYPE; PLANTS, GENETICALLY MODIFIED; PLASTIDS; PROTEIN SUBUNITS; PYRUVATE DEHYDROGENASE COMPLEX; SEEDS;

ARABIDOPSIS; EMBRYOPHYTA; EUKARYOTA; INSERTION SEQUENCES;

EID: 0041693996     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1025076805902     Document Type: Article

References (24)

1. Bao, X., Pollard, M. and Ohlrogge, J. 1998. The biosynthesis of erucic acid in developing embryos of Brassica rapa. Plant Physiol. 118: 183-190.
2. Behal, R.H. and Oliver, D.J. 1999. A second gene encoding the plastidic pyruvate dehydrogenase β-subunit in Arabidopsis thaliana. Plant Physiol. 121: 312-313.
3. Behal, R.H., Lin, M., Back, S. and Oliver, D.J. 2002. Role of acetyl-coenzyme A synthetase in leaves of Arabidopsis thaliana. Arch. Biochem. Biophys. 402: 259-267.
4. Clough, S.J. and Bent, A.F. 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16: 735-743.
5. Drea, S.C., Mould, R.M., Hibberd, J.M., Gray, J.C. and Kavanagh, T.A. 2001. Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex. Plant Mol. Biol. 46: 705-715.
6. Hecht, V., Vielle-Calzada, J.P., Hartog, M.V., Schmidt, E.D., Boutilier, K., Grossniklaus, U. and de Vries, S.C. 2001. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol. 127: 803-816.
7. Hoglund, A.-S., Rodin, J., Larsson, E. and Rask, L. 1992. Distribution of napin and cruciferin in developing rape seed embryos. Plant Physiol. 98: 509-515.
8. Johnston, M.L., Leuthy, M.H., Miernyk, J.A. and Randall, DD. 1997. Cloning and molecular analyses of the Arabidopsis thaliana plastid pyruvate dehydrogenase subunits. Biochim. Biophys. Acta 1321: 200-206.
9. Johnston, M.L., Meirnyk, J.A. and Randall, D.D. 2000. Import, processing, and assembly of the α- and β-subunits of chloroplast pyruvate dehydrogenase. Planta 211: 72-76.
10. Kang, F. and Rawsthorne, S. 1994. Starch and fatty acid synthesis in plastids from developing embryos of oilseed rape. Plant J. 6: 795-805.
11. Kang, F. and Rawsthorne, S. 1996. Metabolism of glucose-6-phosphate and utilization of multiple metabolites for fatty acid synthesis by plastids from developing oilseed rape embryos. Planta 199: 321-327.
12. Ke, J., Choi, J.-K., Smith, M., Horner, H.T., Nikolau, B.J. and Wurtele, E.S. 1997. Structure of the CAC1 gene and in situ characterization of its expression: the Arabidopsis thaliana gene coding for the biotin-containing subunit of the plastidic acetyl-coenzyme A carboxylase. Plant Physiol. 113: 357-365.
13. Ke, J., Behal, R.H., Back, S.L., Nikolau, B.J., Wurtele, E.S. and Oliver, D.J. 2000. The role of pyruvate dehydrogenase and acetyl-coenzyme A synthetase in fatty acid synthesis in developing Arabidopsis seeds. Plant Physiol. 123: 497-508.
14. Lutziger, I. and Oliver, D.J. 2000. Molecular proof of a unique lipoamide dehydrogenase in plastids: analysis of plastidic lipoamide dehydrogenase from Arabidopsis thaliana. FEBS Lett. 484: 12-16.
15. Meinke, D.W. 1985. Embryo-lethal mutants of Arabidopsis thaliana: analysis of mutants with a wide range of lethal phases. Theor. Appl. Genet. 69: 543-552.
16. Meinke, D.W. 1995. Molecular genetics of plant embryogenesis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46: 369-394.
17. Mooney, B.P., Miernyk, J.A. and Randall, D.D. 1999. Cloning and characterization of the dihydrolipoamide S-acetyltransferase subunit of the plastid pyruvate dehydrogenase complex (E2) from Arabidopsis. Plant Physiol. 120: 443-452.
18. Mooney, B.P., Miernyk, J.A. and Randall, D.D. 2002. The complex fate of α-ketoacids. Annu. Rev. Plant Biol. 53: 357-375.
19. Patton, D.A., Schetter, A.L., Franzmann, L.H., Nelson, K., Ward, E.R. and Meinke, D.W. 1998. An embryo-defective mutant of Arabidopsis disrupted in the final step of biotin synthesis. Plant Physiol. 116: 935-946.
20. Schneider, T., Dinkins, R., Robinson, K., Shellhammer, J. and Meinke, D.W. 1989. An embryo-lethal mutant of Arabidopsis thaliana is a biotin auxotroph. Dev. Biol. 131: 161-167.
21. Shellhammer, J. and Meinke, D. 1990. Arrested embryos from the bio1 auxotroph of Arabidopsis thaliana contain reduced levels of biotin. Plant Physiol. 93: 1162-1167.
22. Sun, J., Ke, J., Johnson, J.L., Nikolau, B.J. and Wurtele, E.S. 1997. Biochemical and molecular biological characterization of CAC2, the Arabidopsis thaliana gene coding for the biotin carboxylase subunit of the plastidic acetyl-coenzyme A carboxylase. Plant Physiol. 115: 1371-1383.
23. Sunilkumar, G., Mohr, L., Lopata-Finch, E., Emani, C. and Rathore, K.S. 2002. Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Mol. Biol. 50: 463-474.
24. Thelan, J.J., Muszynski M.G., Miernyk J.A. and Randall, D.D. 1998. Molecular analysis of two pyruvate dehydrogenase kinases from maize. J. Biol. Chem. 273: 26618-26623.