Cell surface labeling of Escherichia coli via copper(I)-catalyzed [3+2] cycloaddition

Journal of the American Chemical Society

Volumn 125, Issue 37, 2003, Pages 11164-11165

  Link, A James ^a   Tirrell, David A ^a  

^a California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COPPER;

ARTICLE; CATALYSIS; CATALYST; CELL SURFACE; CHEMICAL BINDING; CHEMICAL LABELING; CYCLOADDITION; ESCHERICHIA COLI; FLOW CYTOMETRY; NONHUMAN; SITE DIRECTED MUTAGENESIS; STEREOSPECIFICITY; WESTERN BLOTTING;

ALANINE; CATALYSIS; COPPER; ESCHERICHIA COLI; GENE EXPRESSION; MEMBRANE PROTEINS; MODELS, CHEMICAL; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PORINS; STAINING AND LABELING;

EID: 0041692305     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja036765z     Document Type: Article

References (17)

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15. The mutations introduced were V50M, N88M, T187M, A230M, L271M, L306M. The exposed methionine in wild-type OmpC is at residue 142.
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17. Cells subjected to overexpression of either OmpC-met or OmpC-AHA, and then to the [3+2] reaction conditions described here, are unable to divide after transfer to rich medium. Overexpression of recombinant outer membrane proteins is known to adversely affect cell viability (see ref 2 and Christmann et al. Protein Eng. 1999, 12, 797). We are currently exploring conditions for maintaining viability, and for use of nonviable cells in screening experiments.