Mutagenesis of a Gly-Ser cleavage site in MUC1 inhibits ectodomain shedding

Biochemical and Biophysical Research Communications

Volumn 307, Issue 3, 2003, Pages 743-749

  Lillehoj, Erik P ^a   Han, Feng ^a   Kim, K Chul ^a,b  

^a UNIVERSITY OF MARYLAND SCHOOL OF PHARMACY   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Cytoplasmic; Extracellular; Mucin; Proteolysis; Transfection; Transmembrane

Indexed keywords

GLYCINE; MUCIN 1; SERINE;

AMINO ACID SUBSTITUTION; ARTICLE; CELL SURFACE; CONTROLLED STUDY; HUMAN; HUMAN CELL; MUTANT; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

EID: 0041669598     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(03)01260-9     Document Type: Article

References (31)

1. Gendler S.J., Lancaster C.A., Taylor-Papadimitriou J., Duhig T., Peat N., Burchell J., Pemberton L., Lalani E.-N., Wilson D. Molecular cloning and expression of the human tumor associated polymorphic epithelial mucin. J. Biol. Chem. 265:1990;15286-15293.
2. Lan M.S., Batra S.K., Qi W.N., Metzgar R.R.S., Hollingsworth M.A. Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. Biol. Chem. 265:1990;15294-15299.
3. Ligtenberg M.J.L., Vos H.L., Gennissen A.M.C., Hilkens J. Episialin, a carcinoma associated mucin, is generated by a polymorphic gene encoding splice variants with alternative amino termini. J. Biol. Chem. 265:1990;5573-5578.
4. Hilkens J., Ligtenberg M.J., Vos H.L., Litvinov S.V. Cell membrane associated mucins and their adhesion-modulating property. TIBS. 17:1992;359-363.
5. Gendler S.J. MUC1, the renaissance molecule. J. Mammary Gland Biol. Neoplasia. 6:2001;339-353.
6. Parry S., Silverman H.S., McDermott K., Willis A., Hollingsworth M.A., Harris A. Identification of MUC1 proteolytic cleavage sites in vivo. Biochem. Biophys. Res. Commun. 283:2001;715-720.
7. Ligtenberg M.J.L., Kruijshaar L., Buijs F., van Meijer M., Litvinov S.V., Hilkens J. Cell-associated Episialin is a complex containing two proteins derived from a common precursor. J. Biol. Chem. 267:1992;6171-6177.
8. Julian J.A., Carson D.D. Formation of MUC1 metabolic complex is conserved in tumor-derived and normal epithelial cells. Biochem. Biophys. Res. Commun. 293:2002;1183-1190.
9. Thathiah A., Blobel C.P., Carson D.D. Tumor necrosis factor-α converting enzyme (TACE)/ADAM 17 mediates MUC1 shedding. J. Biol. Chem. 278:2003;3386-3394.
10. Boshell M., Lalani E.-N., Pemberton L., Burchell J., Gendler S., Taylor-Papadimitriou J. The product of the human MUC1 gene when secreted by mouse cells transfected with the full-length cDNA lacks the cytoplasmic tail. Biochem. Biophys. Res. Commun. 185:1992;1-8.
11. Pimental R.A., Julian J.A., Gendler S.J., Carson D.D. Synthesis and intracellular trafficking of Muc-1 and mucins by polarized mouse uterine epithelial cells. J. Biol. Chem. 271:1996;28128-28137.
12. Zhang K., Baeckstrom D., Brevinge H., Hansson G.C. Secreted MUC1 mucins lacking their cytoplasmic part and carrying sialyl-Lewis a and x epitopes from a tumor cell line and sera of colon carcinoma patients can inhibit HL-60 leukocyte adhesion to E-selectin-expressing endothelial cell. J. Cell Biochem. 60:1996;538-549.
13. McGuckin M.A., Devine P.L., Ramm L.E., Ward B.G. Factors affecting the measurement of tumor-associated MUC1 mucins in serum. Tumour Biol. 15:1994;3-44.
14. Treon S.P., Maimonis P., Bua D., Young G., Raje N., Mollick J., Chauhan D., Tai Y.T., Hideshima T., Shima Y., Hilgers J., von Mensdorff-Pouilly S., Belch A.R., Pilarshi L.M., Anderson K.C. Elevated soluble MUC1 levels and decreased anti-MUC1 antibody levels in patients with multiple myeloma. Blood. 96:2000;3147-3153.
15. Williams C.J., Wreschner D.H., Tanaka A., Tsarfaty I., Keydar I., Dion A.S. Multiple protein forms of the human mammary tumor-associated epithelial membrane antigen (EMA) are generated by alternative splicing and induced by hormonal stimulation. Biochem. Biophys. Res. Commun. 170:1990;1331-1338.
16. Baruch A., Hartmann M., Yoeli M., Adereth Y., Greenstein S., Stadler Y., Skornik Y., Zaretsky J., Smorodinsky N.I., Keydar I., Wreschner D.H. The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein. Cancer Res. 59:1999;1552-1561.
17. Litvinov S.V., Hilkens J. The epithelial sialomucin, Episialin, is sialyted during recycling. J. Biol. Chem. 268:1993;21364-21371.
18. Meerzaman D., Shapiro P.S., Kim K.C. Involvement of the MAP kinase ERK2 in MUC1 mucin signaling. Am. J. Physiol. Lung Cell. Mol. Physiol. 281:2001;L86-L91.
19. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature. 227:1970;680-685.
21. Pemberton L., Taylor-Papadimitriou J., Gendler S.J. Antibodies to the cytoplasmic domain of the MUC1 mucin show conservation throughout mammals. Biochem. Biophys. Res. Commun. 185:1992;167-175.
22. Meerzaman D., Xing P.X., Kim K.C. Construction and characterization of a chimeric receptor containing the cytoplasmic domain of MUC1 mucin. Am. J. Physiol. Lung. Cell. Mol. Physiol. 278:2000;L625-L629.
23. Wang R., Khatri I.A., Forstner J.F. The carboxyl terminal domain of rodent intestinal Muc3 is proteolytically cleaved in the ER to generate extracellular and membrane components. Biochem. J. 366:2002;623-631.
24. Komatsu M., Arango M.E., Carraway K.L. Synthesis and secretion of Muc4/sialomucin complex: implication of intracellular proteolysis. Biochem. J. 368:2002;41-48.
25. Williams S.J., Wreschner D.H., Tran M., Eyre H.J., Sutherland G.R., McGuckin M.A. MUC13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells. J. Biol. Chem. 276:2001;18327-18336.
26. Bork P., Patthy L. The SEA module: a new extracellular domain associated with O-glycosylation. Prot. Sci. 4:1995;1421-1425.
27. Wreschner D.H., McGuckin M.A., Williams S.J., Baruch A., Yoeli M., Ziv R., Okun L., Zaretsky J., Smorodinsky N., Keydar I., Neophytou P., Stacey M., Lin H.H., Gordon S. Generation of ligand-receptor alliances by "SEA" module-mediated cleavage of membrane-associated mucin proteins. Prot. Sci. 11:2002;698-706.
28. Barnd D.L., Lan M.S., Metzgar R.S., Finn O.J. Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells. Proc. Natl. Acad. Sci. USA. 86:1989;7159-7163.
29. Hayes D.F., Silberstein D.S., Rodrique S.W., Kufe D.W. DF3 antigen, a human epithelial cell mucin, inhibits adhesion of eosinophils to antibody-coated targets. J. Immunol. 145:1990;962-970.
30. Agrawal B., Krantz M.J., Reddish M.A., Longnecker B.M. Cancer-associated MUC1 mucin inhibits human T-cell proliferation, which is reversible by IL-2. Nat. Med. 4:1998;43-49.
31. Chan A.K., Lockhart D.C., von Bernstorff W., Spanjaard R.A., Joo H.G., Eberlein T.J., Godedgebuure P.S. Soluble MUC1 secreted by human epithelial cancer cells mediates immune suppression by blocking T-cell activation. Int. J. Cancer. 82:1999;721-726.