Estimation of cell damage in bench- and pilot-scale affinity expanded- bed chromatography for the purification of monoclonal antibodies

Biotechnology and Bioengineering

Volumn 65, Issue 1, 1999, Pages 114-119

  Lütkemeyer, Dirk ^a   Ameskamp, Nicole ^a   Tebbe, Hermann ^a   Wittler, Justine ^a   Lehmann, Jürgen ^a  

^a BIELEFELD UNIVERSITY   (Germany)

Author keywords

Cell damage; Expanded bed chromatography; Hybridoma; Pilot scale; rProtein A

Indexed keywords

AFFINITY CHROMATOGRAPHY; BIOASSAY; BIODEGRADATION; CELL CULTURE; CELLS; DNA; ENZYMES; MONOCLONAL ANTIBODIES; PH EFFECTS; PURIFICATION; REDUCTION; SEPARATION;

CELL DAMAGE; EXPANDED BED CHROMATOGRAPHY; HYBRIDOMA;

FLUIDIZED BED PROCESS;

IMMUNOGLOBULIN G; LACTATE DEHYDROGENASE; MONOCLONAL ANTIBODY;

ANIMAL CELL; ARTICLE; CELL DAMAGE; CELL VIABILITY; CHROMATOGRAPHY; FLUIDIZED BED; HYBRIDOMA; MOUSE; NONHUMAN; PROTEIN PURIFICATION;

ANIMALS; ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CELL SEPARATION; CELL SURVIVAL; CHROMATOGRAPHY, AFFINITY; DNA; HYBRIDOMAS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; LIGANDS; MICE; PILOT PROJECTS; RECOMBINANT PROTEINS; STAPHYLOCOCCAL PROTEIN A;

ANIMALIA; MAMMALIA;

EID: 0041534765     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19991005)65:1<114::AID-BIT14>3.0.CO;2-P     Document Type: Article

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