Large scale recovery and purification of periplasmic recombinant protein from E. coli using expanded bed adsorption chromatography followed by new ion exchange media

Journal of Biotechnology

Volumn 48, Issue 1-2, 1996, Pages 9-14

  Johansson, H J ^a   Jagersten C ^a   Shiloach, J ^b  

^a GE Healthcare Bio Sciences AB   (Sweden)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

Capture; Chromatography; Exotoxin A; Expanded bed

Indexed keywords

ADSORPTION; BACTERIA; CELLS; CLARIFICATION; ION EXCHANGE MEMBRANES; PACKED BEDS; PROTEINS; PURIFICATION; RECOVERY;

ESCHERICHIA COLI; EXOTOXIN; EXPANDED BED CHROMATOGRAPHY; OSMOTIC SHOCK; PERIPLASMIC RECOMBINANT PROTEIN; PSEUDOMONAS AERUGINOSA;

CHROMATOGRAPHY;

EXOTOXIN A; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; ION EXCHANGE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0030592122     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(96)01390-9     Document Type: Article

References (8)

1. Barnfield Frej, A.K., Hjorth, R. and Hammarstrom, A. (1994) Pilot scale recovery of recombinant annexin V from unclarified E. coli homogenate using expanded bed adsorption. Biotech. Bioeng. 44. 922-929.
2. Chase, H.A. (1994) Purification of proteins by adsorption chromatography in expanded bed. Trends Biotechnol. 12, 296-303.
3. Fass, R., van de Walle, M., Shiloach, A., Joslyn A., Kaufman, J. and Shiloach, J. (1991) Use of high density cultures of E. coli for high level production of recombinant exotoxin A. Appl. Microbiol. Biotechnol. 36, 65-69.
4. Fattom, A., Schneerson, R., Watson, D.C.. Karakawa, W.W., Fitzgerald. D., Pastan, A., Li, X., Shiloach, J., Bryla, D.A. and Robbins, J.B. (1993) Laboratory and clinical evaluation of conjugate vaccines composed of Staphylococcus aerus type 5 and type 8 capsular polysaccharide bound to Pseudomonas aeruginosa recombinant exoprotein A. Infect Immun. 61, 1023-1032.
5. Lukac, M., Pier, G.B. and Collier. R.J. (1988) Toxoid of Pseudomonas aeruginosa exotoxin A generated by an active site residue. Infect Immun. 56, 3095-3098.
6. Mancini, G., Carbonara, A.O. and Hermans, J.F. (1966) Immunological quantitation of antigens by single radial immunodiffusion. Immunochemistry 2, 235-354.
7. Studier, F.W. and Moffat, B.A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned Genes. J. Mol. Biol. 189, 113-130.
8. Tsai, A., Gallo, M., Petterson, T. and Shiloach, J. (1995) Large-scale production and purification of clinical grade Pseudomonas aeruginosa exotoxin A from E. coli. Bioprocess Eng. 12, 115-118.