The Aspergillus nidulans metR gene encodes a bZIP protein which activates transcription of sulphur metabolism genes

Molecular Microbiology

Volumn 49, Issue 4, 2003, Pages 1081-1094

  Natorff, Renata ^a   Sieńko, Marzena ^a   Brzywczy, Jerzy ^a   Paszewski, Andrzej ^a  

^a INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE SYNTHASE; DNA BINDING PROTEIN; FUNGAL ENZYME; GENE PRODUCT; HOMOCYSTEINE; HOMOCYSTEINE SYNTHETASE; METHIONINE; PROTEIN BZIP; PROTEIN METR; SULFATE ADENYLYLTRANSFERASE; SULFATE PERMEASE; SULFUR; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS NIDULANS; DELETION MUTANT; FUNGAL METABOLISM; GENE MUTATION; GENETIC TRANSCRIPTION; INTRON; MOLECULAR RECOGNITION; NEUROSPORA CRASSA; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; REGULATOR GENE; REGULATORY SEQUENCE; TRANSCRIPTION INITIATION;

AMINO ACID SEQUENCE; ASPERGILLUS NIDULANS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; LEUCINE ZIPPERS; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SULFUR; TRANSCRIPTION, GENETIC; ZINC FINGERS;

ASPERGILLUS; EMERICELLA NIDULANS; FUNGI; NEUROSPORA; NEUROSPORA CRASSA;

EID: 0041528539     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2003.03617.x     Document Type: Article

References (48)

1. Arst, H.N., Jr (1968) Genetic analysis of the first steps of sulphate metabolism in Aspergillus nidulans. Nature 219: 268-270.
2. Bai, C., Sen, P., Hofmann, K., Ma, L., Goebl, M., Harper, J.W., and Elledge, S.J. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell 86: 263-274.
3. Borges-Walmsley, M.I., Turner, G., Bailey, A.M., Brown, J., Lehmbeck, J., and Clausen, I.G. (1995) Isolation and characterisation of genes for sulphate activation and reduction in Aspergillus nidulans: implications for evolution of an allosteric control region by gene duplication. Mol Gen Genet 247: 423-429.
4. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantites of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
5. Brody, H., Griffith, J., Cuticchia, A.J., Arnold, J., and Timberlake, W.E. (1991) Chromosome-specific recombinant DNA libraries from the fungus Aspergillus nidulans. Nucleic Acids Res 19: 3105-3109.
6. Burton, E.G., and Metzenberg, R.L. (1972) Novel mutation causing derepression of several enzymes of sulphur metabolism in Neurospora crassa. J Bacteriol 109: 140-151.
7. Chomczyński, P. (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15: 532-537.
8. Clutterbuck, A.J. (1994) Linkage map and locus list. Prog Indust Microbiol 29: 791-824.
9. Cove, D.J. (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochem Biophys Acta 113: 51-56.
10. De Vito, P.C., and Dreyfuss, J. (1964) Metabolic regulation of adenosine triphosphate sulphurylase in yeast. J Bacteriol 88: 1341-1348.
11. Fu, Y.-H., and Marzluf, G.A. (1990) cys-3, the positive-acting sulphur regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein. J Biol Chem 265: 11942-11947.
12. Fu, Y.-H., Paietta, J.V., Mannix, D.G., and Marzluf, G.A. (1989) cys-3, the positive-acting sulphur regulatory gene of Neurospora crassa encodes a protein with a putative leucine zipper DNA-binding element. Mol Cell Biol 9: 1120-1127.
13. Gems, D.H., Johnstone, I.L., and Clutterbuck, A.J. (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98: 61-67.
14. Grynberg, M., Piotrowska, M., Pizzinini, E., Turner, G., and Paszewski, A. (2001) The Aspergillus nidulans metE gene is regulated by a second system, independent from the sulphur metabolite repression. Biochim Biophys Acta 1519: 78-84.
15. Jackson, P.K., Eldridge, A.G., Freed, E., Furstenthal, L., Hsu, J.Y., Kaiser, B.K., and Reimann, J.D.R. (2000) The lore of the RINGs: substrate recognition and catalysis by ubiquitin ligases. Trends Cell Biol 10: 429-439.
16. Met30 mediated inactivation of the transcription factor Met4. Cell 102: 303-314.
17. Kanaan, M.N., Fu, Y.-H., and Marzluf, G.A. (1992) The DNA-binding domain of the CYS3 regulatory protein of Neurospora crassa is bipartite. Biochemistry 31: 3197-3203.
18. Kumar, A., and Paietta, J.V. (1995) The sulphur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with β-transducin repeats. Proc Natl Acad Sci USA 92: 3343-3347.
19. Kuras, L., Cherest, H., Surdin-Kerjan, Y., and Thomas, D. (1996) A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulphur metabolism. EMBO J 15: 2519-2529.
20. Kuras, L., Rouillon, A., Leen, T., Barbey, R., Tyers, M., and Thomas, D. (2002) Dual regulation of the Met4 transcription factor by ubiquitin-dependent degradation and inhibition of promoter recruitment. Molec Cell 10: 69-80.
21. Li, Q., and Marzluf, G.A. (1996) Determination of the Neurospora crassa CYS3 sulphur regulatory protein consensus DNA-binding site: amino-acid substitutions in the CYS3 bZIP domain that alter DNA-binding specificity. Curr Genet 30: 298-304.
22. Martinelli, S.D. (1994) Gene symbols. Media. Prog Indust Microbiol 29: 825-833.
23. Marzluf, G.A. (1997) Molecular genetics of sulphur assimilation in filamentous fungi and yeast. Annu Rev Microbiol 51: 73-96.
24. Marzluf, G.A., and Metzenberg, R.L. (1968) Positive control by the cys-3 locus in regulation of sulphur metabolism in Neurospora. J Mol Biol 33: 423-437.
25. Mountain, H.A., Byström, A.S., and Korch, C. (1993) The general amino acid control regulates MET4, which encodes a methionine-pathway-specific transcriptional activator of Saccharomyces cerevisiae. Mol Microbiol 7: 215-228 (Erratum, 9: 221-223).
26. Natorff, R., Balib́ska, M., and Paszewski, A. (1993) At least four regulatory genes control sulphur metabolite repression in Aspergillus nidulans. Mol Gen Genet 238: 185-192.
27. Natorff, R., Piotrowska, M., and Paszewski, A. (1998) The Aspergillus nidulans sulphur regulatory gene sconB encodes a protein with WD40 repeats and an F-box. Mol Gen Genet 257; 255-263.
28. Paietta, J.V. (1992) Production of the CYS3 regulator, a bZIP DNA-binding protein, is sufficient to induce sulphur gene expression in Neurospora crassa. Mol Cell Biol 12: 1568-1577.
29. Paszewski, A., Natorff, R., Piotrowska, M., Brzywczy, J., Sieńko, M, Grynberg, M., et al. (2000) Regulation of sulphur amino acid biosynthesis in Aspergillus nidulans: physiological and genetical aspects. In Sulphur Nutrition and Sulphur Assimilation in Higher Plants. Brunold, C., Rennenberg, H., De Kok, L.J., Stulen, I., and Davidian, J.-C. (eds). Bern: Paul Haupt Publishers, pp. 93-105.
30. Patton, E.E., Willems, A.R., and Tyers, M. (1998a) Combinatorial control in ubiquitin-dependent proteolysis: don't Skp the F-box hypothesis. Trends Genet 14: 236-243.
31. Patton, E.E., Willems, A.R.SaD., Kuras, L., Thomas, D., Craig, K.L., and Tyers, M (1998b) Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast. Genes Dev 12: 692-705.
32. Met30-mediated control of the transcriptional activator Met4 is required for the G1-S transition. EMBO J 19: 1613-1624.
33. Piotrowska, M., Natorff, R., and Paszewski, A. (2000) The Aspergillus nidulans gene sconC involved in the regulation of sulphur metabolism, belongs to the SKP1 family. Mol Gen Genet 264: 276-282.
34. Pontecorvo, G., Roper, J.A., Hemmons, L.M., McDonald, K.D., and Bufton, A.W.J. (1953) The genetics of Aspergillus nidulans. Adv Genet 5: 141-238.
35. Met30 complex. EMBO J 19: 282-294.
36. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
37. Seol, J.H., Feldman, R.M., Zachariae, W., Shevchenko, A., Correll, C.C., Lyapina, S., et al. (1999) Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Genes Dev 13: 1614-1626.
38. Sieńko, M., Topczewski, J., and Paszewski, A. (1998) Structure and regulation of cysD, the homocysteine synthase gene of Aspergillus nidulans. Curr Genet 33: 136-144.
39. Siegel, L.M. (1965) A direct microdetermination for sulphide. Anal Biochem 11: 126-132.
40. Skowyra, D., Craig, K.L., Tyers, M., Elledge, S.J., and Harper, J.W. (1997) F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex. Cell 91: 209-219.
41. Met30p complex activity and is regulated by methionine availability. Mol Cell Biol 20: 7845-7852.
42. Stevens, C.M., and Vohra, P. (1955) Comparison of utilization of sulfate with choline sulfate for penicillin biosynthesis. J Am Chem Soc 77: 4935-4936.
43. Tao, Y., and Marzluf, G.A. (1998) Synthesis and differential turnover of the CYS3 regulatory protein of Neurospora crassa are subject to sulphur control. J Bacteriol 180: 478-482.
44. Thomas, D., Jacquemin, I., and Surdin-Kerjan, Y. (1992) MET4, a leucine zipper protein, and centromere binding factor 1, are both required for transcriptional activation of sulphur metabolism in Saccharomyces cerevisiae. Mol Cell Biol 12: 1719-1727.
45. Thomas, D., Kuras, L., Barbey, R., Cherest, H., Blaiseau, P.-L., and Surdin-Kerjan, Y. (1995) Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol 15: 6526-6534.
46. Thomas, D., and Surdin-Kerjan, Y. (1997) Metabolism of sulphur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61: 503-532.
47. Topczewski, J., Sieńko, M., and Paszewski, A. (1997) Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase. Curr Genet 31: 348-356.
48. Yelton, M.M., Timberlake, W.E., and van den Hondel, C.A.M.J.J. (1985) A cosmid for selecting genes by complementation in Aspergillus nidulans: selection of the developmentally regulated yA locus. Proc Natl Acad Sci USA 82: 834-838.