Optimized and validated HPLC method for α- and γ-tocopherol measurement in Laurus nobilis leaves. New data on tocopherol content

Journal of Agricultural and Food Chemistry

Volumn 51, Issue 18, 2003, Pages 5196-5201

  Gómez Coronado, Diego J M ^a   Barbas, Coral ^a  

^a UNIVERSIDAD SAN PABLO CEU   (Spain)

Author keywords

Bay; HPLC; Laurus nobilis; Tocopherol; Vitamin E

Indexed keywords

ACETONE; ALPHA TOCOPHEROL; GAMMA TOCOPHEROL;

ACCURACY; ANALYTIC METHOD; ARTICLE; CENTRIFUGATION; ELUTION; EXTRACTION; FILTRATION; FLUORESCENCE; GEOGRAPHIC DISTRIBUTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LAURUS; LAURUS NOBILIS; LINEAR SYSTEM; MICROWAVE OVEN; NONHUMAN; PLANT LEAF; STANDARD; ULTRAVIOLET RADIATION; VALIDATION PROCESS;

ALPHA-TOCOPHEROL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GAMMA-TOCOPHEROL; LAURUS; PLANT LEAVES;

LAURUS; LAURUS NOBILIS;

EID: 0041512889     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf030143f     Document Type: Article

References (17)

1. Chan, A. C. Vitamin E and atherosclerosis. J. Nutr. 1998, 128, 1593-1596.
2. Rimm, E. B.; Stampfer, M. J.; Ascherio, A.; Giovannucci, E.; Colditz, G. A.; Willett, W. C. Vitamin E consumption and the risk of coronary heart disease in men. N. Engl. J. Med. 1993, 328, 1450-1456.
3. Herrera, E.; Barbas, C. Vitamin E: action, metabolism and perspectives. J. Physiol. Biochem. 2001, 57, 43-56.
4. Burton, G. W.; Traber, M. G.; Acuff, R. V.; Walters, D. N.; Kayden, H.; Hughes, L.; Ingold, K. U. Human plasma and tissue α-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E. Am. J. Clin. Nutr. 1998, 67, 669-684.
5. Melchert, H.-U.; Pabel, E. The tocopherol pattern in human serum is markedly influenced by intake of vitamin E drugs - results of the German National Health Surveys. J. Am. Oil Chem. Soc. 1998, 75, 213-216.
6. Rupérez, F. J.; Martín, D.; Herrera, E.; Barbas, C. Chromatograpic analysis of α-tocopherol and related compounds in various matrices. J. Chromatogr. 2001, 935, 45-69.
7. Torre, J.; Lorenzo, M. P.; Martinez-Alcazar, M. P.; Barbas, C. Simple high-performance liquid chromatography method for α-tocopherol measurement in Rosmarinus officinalis leaves. New data on α-tocopherol content. J. Chromatogr. A 2001, 919 (2), 305-311.
8. EURACHEM Guidance Document GD 2 "Accreditation for chemical laboratories: Guidance on the interpretation of the EN45000 series of standards and ISO/IEC; 1993; Guide 25.
9. Fryer, M. J. The antioxidant effects of thylakoid vitamin E (α-tocopherol). Plant Cell Environ. 1992, 12, 381-392.
10. Hess, J. L. Vitamin E, α-tocopherol. In Antioxidants in Higher Plants; Alscher, R. G., Hess, J. L., Eds.; CRC: Boca Raton, FL, 1993.
11. Rupérez, F. J.; Barbas, C.; Castro, M.; Herrera, E. Determination of α-tocopherol and α-tocopheryl acetate in diets of experimental animals. Study of stability in the diets. J. Chromatogr. 1999, 839, 93-99.
12. Ruperez, F. J.; Barbas, C.; Castro, M.; Martinez, S.; Herrera, E. Simplified method for vitamin E determination in rat adipose tissue and mammary glands by high-performance liquid chromatography. J. Chromatogr. A 1998, 823, 483-487.
13. García-Plazaola, J. I.; Becerril, J. M. A rapid high-performance liquid chromatography method to measure lipophilic antioxidants in stressed plants: simultaneous determination of carotenoids and tocopherols. Phytochem. Anal. 1999, 10, 307-313.
14. Marero, L. M.; Homma, S.; Aida, K.; Fujimaki, M. Changes in the tocopherol and unsaturated fatty acid constituents of spices after pasteurization with superheated steam. J. Nutr. Sci. Vitaminol. 1986, 3, 131-136.
15. Demo, A.; Petrakis, C.; Kefalas, P.; Boskou, D. Nutrient antioxidants in some herbs and Mediterranean plant leaves. Food Res. Int. 1998, 31, 351-354.
16. Leipner, J.; Fracheboud, Y.; Stamp, P. Effect of growing season on the photosynthetic apparatus and leaf antioxidative defenses in two maize genotypes of different chilling tolerance. Environ. Exp. Bot. 1999, 42, 129-139.
17. Beecher, G. Establishing compositional data for flavonoids in foods. Crit. Rev. Food Sci. Nutr. 2001, 41, 412.