Detection of dihydrofolate reductase genes by PCR and RFLP

Diagnostic Microbiology and Infectious Disease

Volumn 46, Issue 4, 2003, Pages 295-298

  Navia, Margarita M ^a   Ruiz, Joaquim ^a   Sanchez Cespedes, Javier ^a   Vila, Jordi ^a  

^a INSTITUT D INVESTIGACIONS BIOMÈDIQUES AUGUST PI I SUNYER IDIBAPS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

TRIMETHOPRIM;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; DIHYDROFOLATE REDUCTASE GENE; ESCHERICHIA COLI; GENE; GENE AMPLIFICATION; HYBRIDIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SHIGELLA FLEXNERI; SHIGELLA SONNEI;

EID: 0041424806     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0732-8893(03)00062-2     Document Type: Article

References (11)

1. Adrian P.V., Klugman K.P., Amyes S.G.B. Prevalance of trimethoprim resistant dihydrofolate reductase genes identified with oligonucleotide probes in plasmids from isolates of commensal faecal flora. J Antimicrob Chemother. 35:1995;497-508.
2. Amyes S.G.B., Towner K.J. Trimethoprim resistance epidemiology and molecular aspects . J Med Microbiol. 31:1990;1-19.
3. Fluit A.D.C., Maarten R.V., Schmitz F.-J. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 14:2001;836-871.
4. Guerra B., Soto S.M., Cal S., Mendoza M.C. Antimicrobial resistance and spread of class 1 integrons among Salmonella serotypes. Antimicrob Agents Chemother. 44:2000;2166-2169.
5. Huovinen P., Sundstrom L., Swedberg G., Skold O. Trimethoprim and sulfonamide resistance. Antimicrob Agents Chemother. 39:1995;279-289.
6. Huovinen P. Resistance to trimethoprim-sulfamethoxazole. Clin Infect Dis. 32:2001;1608-1614.
7. Navia M.M., Capitano L., Ruiz J., Vargas M., Urassa H., Schellemberg D., Gascon J., Vila J. Typing and characterization of mechanisms of resistance of Shigella spp. isolated from faeces of children under 5 years of age from Ifakara, Tanzania. J Clin Microbiol. 37:1999;3113-3117.
8. Ruiz J., Marco F., Goñi P., Gallardo F., Mensa J., Trilla A., Jiménez de Anta M.T., Vila J. High frequency of mutations at codon 83 of gyrA gene of quinolone-resistant clinical isolates of Escherichia coli. J Antimicrob Chemother. 36:1995;737-738.
9. Thomson C.J. Trimethoprim and brodimoprim resistance of gram positive and gram negative bacteria. J Chemother. 5:1993;458-464.
10. Tudó G., Gonzalez J., Gatell J.M., Martinez E., García A., Navarro M., Soriano E., Jimenez de Anta M.T. Detection of unsuspected cases of nosocomial transmission of tuberculosis by use of a molecular typing method. Clin Infect Dis. 33:2001;453-459.
11. White P.A., McIver C.J., Deng Y., Rawlinson W.D. Characterisation of two new gene cassettes, aadA5 and dfrA17. FEMS Microbiol Lett. 182:2000;265-269.