Detection of Texas red-labelled double-stranded DNA by non-enzymatic peroxyoxalate chemiluminescence

Luminescence

Volumn 16, Issue 3, 2001, Pages 247-249

  Javier Alba, F ^a   Daban, Joan Ramon ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Chemiluminescent DNA detection; Peroxyoxalate chemiluminescence; TCPO; Texas red

Indexed keywords

CHARGE TRANSFER; CHEMILUMINESCENCE; FLUORESCENCE; REACTION INTERMEDIATES;

(2,4,6-TRICHLOROPHENYL)OXALATE; CHEMILUMINESCENT DNA DETECTION; CHEMILUMINESCENT REACTIONS; DNA BASIS; DNA DETECTION; FLUORESCENT DYES; MINOR GROOVES; NON-ENZYMATIC; PEROXYOXALATE-CHEMILUMINESCENCE; TEXAS RED;

DNA;

DNA; FLUORESCENT DYE; OXALIC ACID DERIVATIVE; PEROXYOXALIC ACID; TEXAS RED; XANTHENE DERIVATIVE;

ARTICLE; CHEMISTRY; CHEMOLUMINESCENCE; ENERGY TRANSFER; SOUTHERN BLOTTING;

BLOTTING, SOUTHERN; CHEMILUMINESCENT MEASUREMENTS; DNA; ENERGY TRANSFER; FLUORESCENT DYES; OXALATES; XANTHENES;

EID: 0041402581     PISSN: 15227235     EISSN: None     Source Type: Journal    
DOI: 10.1002/bio.636     Document Type: Article

References (17)

1. Imai K. Chemiluminescence detection system for high-performance liquid chromatography. Methods Enzymol. 1986; 133: 435-449.
2. Schuster GB. Chemiluminescence of organic peroxides. Conversion of ground-state reactants to excited-state products by the chemically initiated electron-exchange luminescence mechanism. Acc. Chem. Res. 1979; 12: 366-373.
3. Orlovic M, Schowen RL, Givens RS, Alvarez F, Matuszewski B, Parekh N. A simplified model for the dynamics of chemiluminescence in the oxalate-hydrogen peroxide system: toward a reaction mechanism. J. Org. Chem. 1989; 54: 3606-3610.
4. Milofsky RE, Birks JW. Laser photolysis study of the kinetics and mechanism of photoinitiated peroxyoxalate chemiluminescence. J. Am. Chem. Soc. 1991; 113: 9715-9723.
5. Wilson T. Comments on the mechanisms of chemi-and bioluminescence. Photochem. Photobiol. 1995; 62: 601-606.
6. Tseng SS, Mohan AG, Haines LG, Vizcarra LS, Rauhut MM. Efficient peroxyoxalale chemiluminescence from reactions of N-(trifloromethylsulfonyl) oxamides with hydrogen peroxide and fluorescers. J. Org. Chem. 1979; 44: 4113-1116.
7. Dan N, Lau ML, Grayeski ML. Micellar-enhanced aqueous peroxyoxalate chemiluminescence. Anal. Chem. 1991; 63: 1766-1771.
8. Neuvonen H. Kinetics of the decomposition of a chemiluminescent reagent bis(2,4-dinitrophenyl) oxalate in aqueous acetonitrile. J. Chem. Soc. Perkin Trans. 1994; 2: 89-95.
9. Alba FJ, Daban JR. Nonenzymatic chemiluminescent detection and quantitation of total protein on Western and slot blots allowing subsequent immunodetection and sequencing. Electrophoresis 1997; 18: 1960-1966.
10. Alba FJ, Daban JR. Inhibition of peroxyoxalate chemiluminescence by intercalation of fluorescent acceptors between the DNA bases. Photochem. Photobiol. 1999; 69: 405-409.
11. Kuroda N, Hosoki S, Nakashima K, Akiyama S, Givens R. Photographic detection of fluorescent-labelled oligodeoxynucleotide in the blotting format by peroxyoxalate chemiluminescence. J. Biolumin. Chemilumin. 1998; 13: 101-105.
12. Smith LM, Sanders JZ, Kaiser RJ et al. Fluorescent detection in automated DNA sequence analysis. Nature 1986; 321: 674-679.
13. Kricka LJ. Nonisotopic DNA Probe Techniques. Academic Press: San Diego, CA, 1992.
14. Glazer AN, Peck K, Mathies RA. A stable double-stranded DNA-ethidium homodimer complex: application to picogram fluorescence detection of DNA in agarose gels. Proc. Natl. Acad. Sci. USA 1990; 87: 3851-3855.
15. Glazer AN, Rye HS. Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection. Nature 1992; 359: 859-861.
16. Rye HS, Yue S, Wemmer DE et al. Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanide dyes: properties and applications. Nucleic Acids Res. 1992; 20: 2803-2812.
17. Alba FJ, Daban JR. Rapid fluorescent monitoring of total protein patterns on sodium dodecyl sulfate-polyacrylamide gels and Western blots before immunodetection and sequencing. Electrophoresis 1998; 19: 2407-2411.