Nonenzymatic chemiluminescent detection and quantitation of total protein on Western and slot blots allowing subsequent immunodetection and sequencing

Electrophoresis

Volumn 18, Issue 11, 1997, Pages 1960-1966

  Javier Alba, F ^a   Daban, Joan Ramon ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Chemiluminescent protein detection; Immunodetection; N terminal microsequencing; Peroxyoxalate chemiluminescence; Western and slot blotting

Indexed keywords

CHARGE COUPLED DEVICES; CHEMILUMINESCENCE; REACTION INTERMEDIATES; X RAY FILMS;

CHEMILUMINESCENT PROTEIN DETECTION; IMMUNODETECTION; MICROSEQUENCING; N-TERMINAL MICROSEQUENCING; N-TERMINALS; NON-ENZYMATIC; PEROXYOXALATE-CHEMILUMINESCENCE; POLYVINYLIDENE DIFLUORIDE; PROTEIN DETECTION; WESTERN AND SLOT BLOTTING;

PROTEINS;

AMINO ACID SEQUENCE; ARTICLE; CHEMOLUMINESCENCE; FLUORESCENCE; IMMUNOBLOTTING; PROTEIN DETERMINATION;

EID: 0343632350     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150181114     Document Type: Article

References (30)

1. Kricka, L. J., Clin. Chem. 1991, 37, 1472-1481.
2. Nicolas, J. C., J. Biolumin. Chemilumin. 1994, 9, 139-144.
3. Kricka, L., J., Anal. Chem. 1995, 67, 499R-502R.
4. Mansfield, E. S., Worley, J. M., McKenzie, S. E., Surrey, S., Rappaport, E., Fortina, P., Mol. Cell Probes 1995, 9, 145-156.
5. Kricka, L. J., in: Diamandis, E. P., Christopoulos, T. K., (Eds.), Immunoassay Academic Press, New York 1996, pp. 337-357.
6. Thorpe, G. H. G., Kricka, L. J., Methods Enzymol. 1986, 133, 331-354.
7. Schaap, A. P., Akhavan, H., Romano, L., J., Clin. Chem. 1989, 35, 1863-1864.
8. Bronstein, I., McGrath, P., Nature 1989, 338, 599-600.
9. Bronstein, I., Voyta, J. C., Murphy, O. J., Bresnick, L., Kricka, L. J., BioTechniques 1992, 12, 748-753.
10. Olesen, C. E. M., Martin, C. S., Bronstein, I., BioTechniques 1993, 15, 480-485.
11. Vesterberg, O., Acevedo, F., Bayard, C., Electrophoresis 1995, 16, 1390-1393.
12. Imai, K., Methods Enzymol. 1986, 133, 435-449.
13. Grayeski, M. L., DeVasto, J. K., Anal. Chem. 1987, 59, 1203-1206.
14. Orlovic, M., Schowen, R. L., Givens, R. S., Alvarez, F., Matuszewski, B., Parekh, N., J. Org. Chem. 1989, 54, 3606-3610.
15. Milofsky, R. E., Birks, J. W., J. Amer. Chem. Soc. 1991, 113, 9715-9723.
16. Imai, K., Nawa, H., Tanaka, M., Ogata, H., Analyst 1986, 111, 209-211.
17. Tseng, S.-S., Mohan, A. G., Haines, L. G., Vizcarra, L., Raubut, M. M., J. Org. Chem. 1979, 44, 4143-4116.
18. Dan, N., Lau, M. L., Grayeski, M. L., Anal. Chem. 1991, 63, 1766-1771.
19. Oh, S. K., Cha, S. H., Anal. Biochem. 1994, 218, 222-224.
20. Neuvonen, H., J. Chem. Soc. Perkin Trans. 2 1994, 89-95.
21. Weigele, M., De Bernardo, S., Leimgruber, W., Cleeland, R., Grunberg, E., Biochem. Biophys. Res. Comm. 1973, 54, 899-906.
22. Urwin, V. E., Jackson, P., Anal. Biochem. 1991, 195, 30-37.
23. Daban, J.-R., Bartolomé, S., Samsó, M., Anal. Biochem. 1991, 199, 169-174.
24. Daban, J.-R., Cantor, C. R., J. Mol. Biol. 1982, 156, 749-769.
25. Kwakman, P. J. M., Koelewijn, H., Kool, I., Brinkman, U. A., T., De Jong, G. J., J. Chromatogr. 1990, 511, 155-166.
26. Nguyen, Q., Heffelfinger, D. M., Anal. Biochem. 1995, 226, 59-47.
27. Mann, B., Grayeski, M. L., Anal. Chem. 1990, 62, 1532-1536.
28. Cepas, J., Silva, M., Pérez-Bendito, D., Anal. Chem. 1994, 66, 4079-4084.
29. Summers, D. F., Szewczyk, B., in: Walker, J. M. (Ed.), The Protein Protocols Handbook, Humana Press, Totowa 1996, pp. 289-292.
30. Guy, G., in: Walker, J. M. (Ed.), The Protein Protocols Handbook, Humana Press, Totowa 1996, pp., 329-335.