Analysis of phosphatidylcholine and sphingomyelin molecular species from brain extracts using capillary liquid chromatography electrospray ionization mass spectrometry

Journal of Neuroscience Methods

Volumn 128, Issue 1-2, 2003, Pages 111-119

  Isaac, Giorgis ^a   Bylund, Dan ^a   Månsson, Jan Eric ^b   Markides, Karin E ^a   Bergquist, Jonas ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Electrospray ionization; Lipidomics; Liquid chromatography; Mass spectrometry; Phosphatidylcholine; Phospholipids; Pressurized fluid extraction; Sphingomyelin

Indexed keywords

BRAIN EXTRACT; PHOSPHATIDYLCHOLINE; SPHINGOMYELIN;

AGED; ARTICLE; CELL COMPOSITION; CONTROLLED STUDY; ELUTION; EXTRACTION; FEMALE; HUMAN; HUMAN TISSUE; LIPID ANALYSIS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PRESSURE; PRIORITY JOURNAL; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TISSUE;

EID: 0041387451     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(03)00168-7     Document Type: Article

References (17)

1. Brouwers J.F.H.M., Gadella B.M., Van Golde L.M.G., Tielens A.G.M. Quantitative analysis of phosphatidylcholine molecular species using HPLC and light scattering detection. J. Lipid Res. 39:1998;344-353.
2. Brugger B., Erben G., Sandhoff R., Wieland F.T., Lehmann W.D. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc. Natl. Acad. Sci. USA. 94:(6):1997;2339-2344.
3. Careri M., Mangia A., Musci M. Overview of the application of liquid chromatography-mass spectrometry interfacing system in food analysis: naturally occurring substances in food. J. Chromatogr. A. 794:1998;263-297.
4. Carrier A., Parent J., Dupuis S. Quantitation and characterization of phospholipids in pharmaceutical formulations by liquid chromatography-mass spectrometry. J. Chromatogr. A. 876:2000;97-109.
5. Fang J., Barcelona M.J. Structural determination and quantitative analysis of bacterial phospholipids using liquid chromatography/electrospray ionization mass spectrometry. J. Microbiol. Methods. 33:1998;23-35.
6. Fenton W.S., Hibbeln J., Knable M. Essential fatty acids, lipid membrane abnormalities, and the diagnosis and treatment of schizophrenia. Biol. Psychiatry. 47:2000;8-21.
7. Fillet M., Van Heugen J.C., Servais A.C., De Graeve J., Crommen J. Separation, identification and quantitation of ceramides in human cancer cells by liquid chromatography-electrospray ionization tandem mass spectrometry. J. Chromatogr. A. 949:2002;225-233.
8. Folch J., Lees M., Stanley G.H.S. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 226:1957;497-509.
9. Hsu F.F., Turk J. Structural determination of sphingomyelin by tandem mass spectrometry with electrospray ionization. J. Am. Soc. Mass Spectrom. 11:2000;437-449.
10. Hsu F.F., Bohrer A., Turk J. Formation of lithiated adducts of glycerophosphocholine lipids facilitates their identification by electrospray ionization tandem mass spectrometry. J. Am. Soc. Mass Spectrom. 9:1998;516-526.
11. Karlsson A.Å., Michelsen P., Odham G. Molecular species of sphingomyelin: determination by high-performance liquid chromatography/mass spectrometry with electrospray and high-performance liquid chromatography/ tandem mass spectrometry with atmospheric pressure chemical ionization. J. Mass Spectrom. 33:1998;1192-1198.
12. Kerwin J.L., Tuininga A.R., Ericsson L.H. Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry. J. Lipid Res. 35:1994;1102-1114.
13. Kim H.Y., Wang T.C.L., Ma Y.C. Liquid chromatography/mass spectrometry of phospholipids using electrospray ionization. Anal. Chem. 66:1994;3977-3982.
14. Mano N., Oda Y., Yamada K., Asakawa N., Katayama K. Simultaneous quantitative determination method for sphingolipid metabolites by liquid chromatography/ionspray ionization tandem mass spectrometry. Anal. Biochem. 244:1997;291-300.
15. Murphy R.C., Fiedler J., Hevko J. Analysis of nonvolatile lipids by mass spectrometry. Chem. Rev. 101:2001;479-526.
16. Olsson N.U., Salem N. Jr. Molecular species analysis of phospholipids. J. Chromatogr. B. 692:1997;245-256.
17. Vernooij E.A.A.M., Brouwers J.F.H.M., Bosch J.J.K., Crommelin D.J.A. RP-HPLC/ESI MS determination of acyl chain positions in phospholipids. J. Sep. Sci. 25:2002;285-289.